Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers
Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction techniq...
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| Veröffentlicht in: | Journal of invertebrate pathology 1998-07, Vol.72 (1), p.21-27 |
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| creator | ALIPPI, A. M AGUILAR, O. M |
| description | Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction technique (PCR) with BOX sequence-specific primers. Isolates from Argentina generated three groups of patterns (designated A, B, and C), while P. larvae subsp. larvae strains obtained from other countries yielded two distinguishable patterns (coincident with A and B). Strains from U.S. A. and Germany were identical and related to Group A, while all Czech and English isolates belonged to Group B. Strains from France, Poland, Italy, Sweden, and New Zealand showed two different patterns (A and B). Comparisons of the biochemical type and genotype of isolates rendered no obvious linkage between both features. These results suggest that AFB in Argentina resulted from multiple sources of contaminated material. |
| doi_str_mv | 10.1006/jipa.1998.4748 |
| format | Article |
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M ; AGUILAR, O. M</creator><creatorcontrib>ALIPPI, A. M ; AGUILAR, O. M</creatorcontrib><description>Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction technique (PCR) with BOX sequence-specific primers. Isolates from Argentina generated three groups of patterns (designated A, B, and C), while P. larvae subsp. larvae strains obtained from other countries yielded two distinguishable patterns (coincident with A and B). Strains from U.S. A. and Germany were identical and related to Group A, while all Czech and English isolates belonged to Group B. Strains from France, Poland, Italy, Sweden, and New Zealand showed two different patterns (A and B). Comparisons of the biochemical type and genotype of isolates rendered no obvious linkage between both features. These results suggest that AFB in Argentina resulted from multiple sources of contaminated material.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1006/jipa.1998.4748</identifier><identifier>PMID: 9647697</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Animal productions ; Animals ; Apiculture ; Bacillaceae - genetics ; Bacillaceae - isolation & purification ; Bees - microbiology ; Biological and medical sciences ; DNA Fingerprinting ; DNA Primers ; DNA, Bacterial ; Fundamental and applied biological sciences. 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M</creatorcontrib><title>Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction technique (PCR) with BOX sequence-specific primers. Isolates from Argentina generated three groups of patterns (designated A, B, and C), while P. larvae subsp. larvae strains obtained from other countries yielded two distinguishable patterns (coincident with A and B). Strains from U.S. A. and Germany were identical and related to Group A, while all Czech and English isolates belonged to Group B. Strains from France, Poland, Italy, Sweden, and New Zealand showed two different patterns (A and B). Comparisons of the biochemical type and genotype of isolates rendered no obvious linkage between both features. These results suggest that AFB in Argentina resulted from multiple sources of contaminated material.</description><subject>Animal productions</subject><subject>Animals</subject><subject>Apiculture</subject><subject>Bacillaceae - genetics</subject><subject>Bacillaceae - isolation & purification</subject><subject>Bees - microbiology</subject><subject>Biological and medical sciences</subject><subject>DNA Fingerprinting</subject><subject>DNA Primers</subject><subject>DNA, Bacterial</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Paenibacillus larvae larvae</subject><subject>Polymerase Chain Reaction</subject><subject>Terrestrial animal productions</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtP6zAQhS10EZTHlh2SF1fsUuzEz-W9FS8JCRYgsavGjtMauXGwk0rld_CDSUVhy2rOzPk0ozkInVEypYSIy1ffwZRqraZMMrWHJpRoURBF-B80IaQsi5JQeoiOcn4lo-JCH6ADLZgUWk7Qx2wJCWzvkn-H3scWxwb7HAP0Lm_1I7jWG7A-hCHjAGkNDufB5G763TUprnDt1y5lhxcuLhJ0S28h4Jj8wrfYbHC_dLiLYbNyCUbKLmGcJzde3t6Etsb_H15wl_wI5BO030DI7nRXj9Hz9dXT7La4f7i5m_27LzqqVV9oI4yRlDWW1zXTptSU80YxAaBqyUnDqKLMci4lkVBq09QV8JooSoUVrqyO0cXX3i7Ft8Hlfr7y2boQoHVxyHOptZRSq19BKjjXla5G8HwHDmbl6vn2IUib-S7v0f-78yGPATUJWuvzD1ZWqqIlqz4BqjORpQ</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>ALIPPI, A. M</creator><creator>AGUILAR, O. 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M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p198t-9b6bb714fc5dd49b29155f846aa8d750f41814c557707a29bfd3a5d08116c6e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>Apiculture</topic><topic>Bacillaceae - genetics</topic><topic>Bacillaceae - isolation & purification</topic><topic>Bees - microbiology</topic><topic>Biological and medical sciences</topic><topic>DNA Fingerprinting</topic><topic>DNA Primers</topic><topic>DNA, Bacterial</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Paenibacillus larvae larvae</topic><topic>Polymerase Chain Reaction</topic><topic>Terrestrial animal productions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ALIPPI, A. M</creatorcontrib><creatorcontrib>AGUILAR, O. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ALIPPI, A. M</au><au>AGUILAR, O. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>72</volume><issue>1</issue><spage>21</spage><epage>27</epage><pages>21-27</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>Ninety-nine strains of Paenibacillus larvae subsp. larvae, the causal agent of American Foulbrood disease (AFB) of honeybees, were isolated from different regions of Argentina and other countries. The isolates were characterized on the basis of DNA fingerprints by a polymerase chain reaction technique (PCR) with BOX sequence-specific primers. Isolates from Argentina generated three groups of patterns (designated A, B, and C), while P. larvae subsp. larvae strains obtained from other countries yielded two distinguishable patterns (coincident with A and B). Strains from U.S. A. and Germany were identical and related to Group A, while all Czech and English isolates belonged to Group B. Strains from France, Poland, Italy, Sweden, and New Zealand showed two different patterns (A and B). Comparisons of the biochemical type and genotype of isolates rendered no obvious linkage between both features. These results suggest that AFB in Argentina resulted from multiple sources of contaminated material.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>9647697</pmid><doi>10.1006/jipa.1998.4748</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0022-2011 |
| ispartof | Journal of invertebrate pathology, 1998-07, Vol.72 (1), p.21-27 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal productions Animals Apiculture Bacillaceae - genetics Bacillaceae - isolation & purification Bees - microbiology Biological and medical sciences DNA Fingerprinting DNA Primers DNA, Bacterial Fundamental and applied biological sciences. Psychology Insecta Invertebrates Paenibacillus larvae larvae Polymerase Chain Reaction Terrestrial animal productions |
| title | Characterization of isolates of Paenibacillus larvae subsp. larvae from diverse geographical origin by the polymerase chain reaction and BOX primers |
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