Baseline Diene Conjugation in LDL Lipids as a Direct Measure of In Vivo LDL Oxidation
Objectives: To additionally test validity of the recently developed method (LDL baseline diene conjugation, LDL-BDC) for determination of circulating oxidized LDL. Design and methods: A detailed comparison between the ultracentrifugation and heparin precipitation methods for LDL isolation was perfor...
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| Veröffentlicht in: | Clinical biochemistry 1998-06, Vol.31 (4), p.257-261 |
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| container_title | Clinical biochemistry |
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| creator | Ahotupa, Markku Marniemi, Jukka Lehtimäki, Terho Talvinen, Kati Raitakari, Olli T. Vasankari, Tommi Viikari, Jorma Luoma, Jukka Ylä-Herttuala, Seppo |
| description | Objectives: To additionally test validity of the recently developed method (LDL baseline diene conjugation, LDL-BDC) for determination of circulating oxidized LDL.
Design and methods: A detailed comparison between the ultracentrifugation and heparin precipitation methods for LDL isolation was performed to test suitability of the fast precipitation method. Validity of LDL-BDC as an indicator of circulating oxidized LDL was tested by comparing LDL-BDC to results obtained by the immunological autoantibody method.
Results: BDC values in LDL isolated by heparin precipitation did not differ from those isolated by sequential ultracentrifugation. While highest amount of diene conjugation was found in LDL (40% of that in serum), substantial amounts were also found in VLDL (31%) and HDL (25%). When analyzed in the same samples, assays for the titer of autoantibodies against oxidized LDL and LDL-BDC were found to show good correlation (
r = 0.57,
p = 0.001,
n = 29).
Conclusions: These results, together with thus far conducted studies on clinical applicability of the method, indicate that LDL-BDC is a promising candidate in search for a method for estimation of LDL oxidation
in vivo. |
| doi_str_mv | 10.1016/S0009-9120(98)00018-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79974494</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0009912098000186</els_id><sourcerecordid>79974494</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-a227e337aa5a610c7258728a5ed31e6d079cb7995267017cfb41878537e6a5993</originalsourceid><addsrcrecordid>eNqFkF1LwzAUhoMoc378hEGuRC-qSdMmzZXo5segsgudtyFLTyWja2fSDv33Zt3YrRASDud5czgPQiNKbimh_O6dECIjSWNyLbObUNAs4kdoSDPBolgydoyGB-QUnXm_DGWcZHyABpInXCZyiOaP2kNla8ATC-EeN_Wy-9KtbWpsa5xPcpzbtS081uEEyIFp8Rto3znATYmnNf60m6YnZz-26KMX6KTUlYfL_XuO5s9PH-PXKJ-9TMcPeWQYJ22k41gAY0LrVHNKjIjTTMSZTqFgFHhBhDQLIWUac0GoMOUiCdtlKRPAdSolO0dXu3_XrvnuwLdqZb2BqtI1NJ1XISuSRCYBTHegcY33Dkq1dnal3a-iRG11ql6n2rpSMlO9TsVDbrQf0C1WUBxSe3-hf7_rQ9hyY8Epb4JHA0UvShWN_WfCH-ImgZQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79974494</pqid></control><display><type>article</type><title>Baseline Diene Conjugation in LDL Lipids as a Direct Measure of In Vivo LDL Oxidation</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Ahotupa, Markku ; Marniemi, Jukka ; Lehtimäki, Terho ; Talvinen, Kati ; Raitakari, Olli T. ; Vasankari, Tommi ; Viikari, Jorma ; Luoma, Jukka ; Ylä-Herttuala, Seppo</creator><creatorcontrib>Ahotupa, Markku ; Marniemi, Jukka ; Lehtimäki, Terho ; Talvinen, Kati ; Raitakari, Olli T. ; Vasankari, Tommi ; Viikari, Jorma ; Luoma, Jukka ; Ylä-Herttuala, Seppo</creatorcontrib><description>Objectives: To additionally test validity of the recently developed method (LDL baseline diene conjugation, LDL-BDC) for determination of circulating oxidized LDL.
Design and methods: A detailed comparison between the ultracentrifugation and heparin precipitation methods for LDL isolation was performed to test suitability of the fast precipitation method. Validity of LDL-BDC as an indicator of circulating oxidized LDL was tested by comparing LDL-BDC to results obtained by the immunological autoantibody method.
Results: BDC values in LDL isolated by heparin precipitation did not differ from those isolated by sequential ultracentrifugation. While highest amount of diene conjugation was found in LDL (40% of that in serum), substantial amounts were also found in VLDL (31%) and HDL (25%). When analyzed in the same samples, assays for the titer of autoantibodies against oxidized LDL and LDL-BDC were found to show good correlation (
r = 0.57,
p = 0.001,
n = 29).
Conclusions: These results, together with thus far conducted studies on clinical applicability of the method, indicate that LDL-BDC is a promising candidate in search for a method for estimation of LDL oxidation
in vivo.</description><identifier>ISSN: 0009-9120</identifier><identifier>EISSN: 1873-2933</identifier><identifier>DOI: 10.1016/S0009-9120(98)00018-6</identifier><identifier>PMID: 9646949</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; atherosclerosis ; Chemical Precipitation ; Female ; Heparin - metabolism ; human serum ; Humans ; in vivo ; lipid peroxidation ; Lipoproteins, LDL - blood ; Lipoproteins, LDL - immunology ; Lipoproteins, VLDL - blood ; low-density lipoprotein ; Male ; oxidation ; Oxidation-Reduction ; Reagent Kits, Diagnostic ; Reproducibility of Results ; Ultracentrifugation - methods</subject><ispartof>Clinical biochemistry, 1998-06, Vol.31 (4), p.257-261</ispartof><rights>1998 Elsevier Science Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-a227e337aa5a610c7258728a5ed31e6d079cb7995267017cfb41878537e6a5993</citedby><cites>FETCH-LOGICAL-c360t-a227e337aa5a610c7258728a5ed31e6d079cb7995267017cfb41878537e6a5993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0009912098000186$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9646949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahotupa, Markku</creatorcontrib><creatorcontrib>Marniemi, Jukka</creatorcontrib><creatorcontrib>Lehtimäki, Terho</creatorcontrib><creatorcontrib>Talvinen, Kati</creatorcontrib><creatorcontrib>Raitakari, Olli T.</creatorcontrib><creatorcontrib>Vasankari, Tommi</creatorcontrib><creatorcontrib>Viikari, Jorma</creatorcontrib><creatorcontrib>Luoma, Jukka</creatorcontrib><creatorcontrib>Ylä-Herttuala, Seppo</creatorcontrib><title>Baseline Diene Conjugation in LDL Lipids as a Direct Measure of In Vivo LDL Oxidation</title><title>Clinical biochemistry</title><addtitle>Clin Biochem</addtitle><description>Objectives: To additionally test validity of the recently developed method (LDL baseline diene conjugation, LDL-BDC) for determination of circulating oxidized LDL.
Design and methods: A detailed comparison between the ultracentrifugation and heparin precipitation methods for LDL isolation was performed to test suitability of the fast precipitation method. Validity of LDL-BDC as an indicator of circulating oxidized LDL was tested by comparing LDL-BDC to results obtained by the immunological autoantibody method.
Results: BDC values in LDL isolated by heparin precipitation did not differ from those isolated by sequential ultracentrifugation. While highest amount of diene conjugation was found in LDL (40% of that in serum), substantial amounts were also found in VLDL (31%) and HDL (25%). When analyzed in the same samples, assays for the titer of autoantibodies against oxidized LDL and LDL-BDC were found to show good correlation (
r = 0.57,
p = 0.001,
n = 29).
Conclusions: These results, together with thus far conducted studies on clinical applicability of the method, indicate that LDL-BDC is a promising candidate in search for a method for estimation of LDL oxidation
in vivo.</description><subject>Adult</subject><subject>atherosclerosis</subject><subject>Chemical Precipitation</subject><subject>Female</subject><subject>Heparin - metabolism</subject><subject>human serum</subject><subject>Humans</subject><subject>in vivo</subject><subject>lipid peroxidation</subject><subject>Lipoproteins, LDL - blood</subject><subject>Lipoproteins, LDL - immunology</subject><subject>Lipoproteins, VLDL - blood</subject><subject>low-density lipoprotein</subject><subject>Male</subject><subject>oxidation</subject><subject>Oxidation-Reduction</subject><subject>Reagent Kits, Diagnostic</subject><subject>Reproducibility of Results</subject><subject>Ultracentrifugation - methods</subject><issn>0009-9120</issn><issn>1873-2933</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LwzAUhoMoc378hEGuRC-qSdMmzZXo5segsgudtyFLTyWja2fSDv33Zt3YrRASDud5czgPQiNKbimh_O6dECIjSWNyLbObUNAs4kdoSDPBolgydoyGB-QUnXm_DGWcZHyABpInXCZyiOaP2kNla8ATC-EeN_Wy-9KtbWpsa5xPcpzbtS081uEEyIFp8Rto3znATYmnNf60m6YnZz-26KMX6KTUlYfL_XuO5s9PH-PXKJ-9TMcPeWQYJ22k41gAY0LrVHNKjIjTTMSZTqFgFHhBhDQLIWUac0GoMOUiCdtlKRPAdSolO0dXu3_XrvnuwLdqZb2BqtI1NJ1XISuSRCYBTHegcY33Dkq1dnal3a-iRG11ql6n2rpSMlO9TsVDbrQf0C1WUBxSe3-hf7_rQ9hyY8Epb4JHA0UvShWN_WfCH-ImgZQ</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Ahotupa, Markku</creator><creator>Marniemi, Jukka</creator><creator>Lehtimäki, Terho</creator><creator>Talvinen, Kati</creator><creator>Raitakari, Olli T.</creator><creator>Vasankari, Tommi</creator><creator>Viikari, Jorma</creator><creator>Luoma, Jukka</creator><creator>Ylä-Herttuala, Seppo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980601</creationdate><title>Baseline Diene Conjugation in LDL Lipids as a Direct Measure of In Vivo LDL Oxidation</title><author>Ahotupa, Markku ; Marniemi, Jukka ; Lehtimäki, Terho ; Talvinen, Kati ; Raitakari, Olli T. ; Vasankari, Tommi ; Viikari, Jorma ; Luoma, Jukka ; Ylä-Herttuala, Seppo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-a227e337aa5a610c7258728a5ed31e6d079cb7995267017cfb41878537e6a5993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>atherosclerosis</topic><topic>Chemical Precipitation</topic><topic>Female</topic><topic>Heparin - metabolism</topic><topic>human serum</topic><topic>Humans</topic><topic>in vivo</topic><topic>lipid peroxidation</topic><topic>Lipoproteins, LDL - blood</topic><topic>Lipoproteins, LDL - immunology</topic><topic>Lipoproteins, VLDL - blood</topic><topic>low-density lipoprotein</topic><topic>Male</topic><topic>oxidation</topic><topic>Oxidation-Reduction</topic><topic>Reagent Kits, Diagnostic</topic><topic>Reproducibility of Results</topic><topic>Ultracentrifugation - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahotupa, Markku</creatorcontrib><creatorcontrib>Marniemi, Jukka</creatorcontrib><creatorcontrib>Lehtimäki, Terho</creatorcontrib><creatorcontrib>Talvinen, Kati</creatorcontrib><creatorcontrib>Raitakari, Olli T.</creatorcontrib><creatorcontrib>Vasankari, Tommi</creatorcontrib><creatorcontrib>Viikari, Jorma</creatorcontrib><creatorcontrib>Luoma, Jukka</creatorcontrib><creatorcontrib>Ylä-Herttuala, Seppo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahotupa, Markku</au><au>Marniemi, Jukka</au><au>Lehtimäki, Terho</au><au>Talvinen, Kati</au><au>Raitakari, Olli T.</au><au>Vasankari, Tommi</au><au>Viikari, Jorma</au><au>Luoma, Jukka</au><au>Ylä-Herttuala, Seppo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Baseline Diene Conjugation in LDL Lipids as a Direct Measure of In Vivo LDL Oxidation</atitle><jtitle>Clinical biochemistry</jtitle><addtitle>Clin Biochem</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>31</volume><issue>4</issue><spage>257</spage><epage>261</epage><pages>257-261</pages><issn>0009-9120</issn><eissn>1873-2933</eissn><abstract>Objectives: To additionally test validity of the recently developed method (LDL baseline diene conjugation, LDL-BDC) for determination of circulating oxidized LDL.
Design and methods: A detailed comparison between the ultracentrifugation and heparin precipitation methods for LDL isolation was performed to test suitability of the fast precipitation method. Validity of LDL-BDC as an indicator of circulating oxidized LDL was tested by comparing LDL-BDC to results obtained by the immunological autoantibody method.
Results: BDC values in LDL isolated by heparin precipitation did not differ from those isolated by sequential ultracentrifugation. While highest amount of diene conjugation was found in LDL (40% of that in serum), substantial amounts were also found in VLDL (31%) and HDL (25%). When analyzed in the same samples, assays for the titer of autoantibodies against oxidized LDL and LDL-BDC were found to show good correlation (
r = 0.57,
p = 0.001,
n = 29).
Conclusions: These results, together with thus far conducted studies on clinical applicability of the method, indicate that LDL-BDC is a promising candidate in search for a method for estimation of LDL oxidation
in vivo.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9646949</pmid><doi>10.1016/S0009-9120(98)00018-6</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9120 |
| ispartof | Clinical biochemistry, 1998-06, Vol.31 (4), p.257-261 |
| issn | 0009-9120 1873-2933 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult atherosclerosis Chemical Precipitation Female Heparin - metabolism human serum Humans in vivo lipid peroxidation Lipoproteins, LDL - blood Lipoproteins, LDL - immunology Lipoproteins, VLDL - blood low-density lipoprotein Male oxidation Oxidation-Reduction Reagent Kits, Diagnostic Reproducibility of Results Ultracentrifugation - methods |
| title | Baseline Diene Conjugation in LDL Lipids as a Direct Measure of In Vivo LDL Oxidation |
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