Production of Adrenomedullin in Macrophage Cell Line and Peritoneal Macrophage

We demonstrate that adrenomedullin (AM) is produced and secreted from cultured murine monocyte/macrophage cell line (RAW 264.7) as well as mouse peritoneal macrophage. Immunoreactive (IR) AM secreted from RAW 264.7 cells was chromatographically identified to be native AM. To elucidate the regulation...

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Veröffentlicht in:	The Journal of biological chemistry 1998-07, Vol.273 (27), p.16730-16738
Hauptverfasser:	Kubo, A, Minamino, N, Isumi, Y, Katafuchi, T, Kangawa, K, Dohi, K, Matsuo, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenomedullin Animals Base Sequence Cell Line Chromatography, Gel Chromatography, High Pressure Liquid Chromatography, Ion Exchange Culture Media, Conditioned DNA Primers Interleukin-6 - genetics Macrophages - metabolism Macrophages, Peritoneal - metabolism Mice Mice, Inbred BALB C Peptides - genetics Peptides - isolation & purification Peptides - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Transcription, Genetic Tumor Necrosis Factor-alpha - genetics
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