Preparation of Monoclonal Antibodies against N-(γ-Maleimidobutyryloxy)succinimide (GMBS)-Conjugated Acetylspermine, and Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for N1,N12-Diacetylspermine

We have developed three mouse monoclonal antibodies (mAb) of types IgG1 and IgG2h, i.e. anti-acetylspermine (Ac-Spm)-1 and 2 (ACSPM-1 and 2), and anti-acetylspermine (Ac-Spm)-3 (ACSPM-3), respectively, against Ac-Spm conjugated to bovine serum albumin via a heterobifunctional cross-linker, N-(γ-male...

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