A hyperpolarization-activated cation current (Ih) contributes to resting membrane potential in rat superior cervical sympathetic neurones
Using perforated-patch voltage-clamp recording, a prominent hyperpolarization-activated inward cation current (Ih) has been identified in dissociated, cultured and replated, superior cervical sympathetic (SCG) neurones from 17-day-old rats. Ih was identified as a slowly activated inward current on h...
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| Veröffentlicht in: | Pflügers Archiv 1998-08, Vol.436 (3), p.429-435 |
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| description | Using perforated-patch voltage-clamp recording, a prominent hyperpolarization-activated inward cation current (Ih) has been identified in dissociated, cultured and replated, superior cervical sympathetic (SCG) neurones from 17-day-old rats. Ih was identified as a slowly activated inward current on hyperpolarizing from -60 mV, with an extrapolated null potential (in 3 mM [K+]out) of -42 mV. The activation range for Ih was -40 to -100 mV, with a half-activation voltage (V0.5) of -63 mV. The current was suppressed by 1 mM Cs+ but not by 1 mM Ba2+. The reversal potential for the current change induced by Cs+ agreed with the null potential for Ih. Ih conferred strong inward rectification to the current-voltage curve negative to -55 mV in both voltage-clamp and current-clamp recording. This inward rectification was reduced by 1 mM Cs+. In a sample of eight cells with initial resting membrane potentials between -51 and -64 mV, Cs+ increased the resting potential of all cells by between 2.5 and 21 mV. These results indicate that Ih contributes a tonic inward (depolarizing) component to the maintenance of the resting membrane potential in SCG neurones. |
| doi_str_mv | 10.1007/s004240050653 |
| format | Article |
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Ih was identified as a slowly activated inward current on hyperpolarizing from -60 mV, with an extrapolated null potential (in 3 mM [K+]out) of -42 mV. The activation range for Ih was -40 to -100 mV, with a half-activation voltage (V0.5) of -63 mV. The current was suppressed by 1 mM Cs+ but not by 1 mM Ba2+. The reversal potential for the current change induced by Cs+ agreed with the null potential for Ih. Ih conferred strong inward rectification to the current-voltage curve negative to -55 mV in both voltage-clamp and current-clamp recording. This inward rectification was reduced by 1 mM Cs+. In a sample of eight cells with initial resting membrane potentials between -51 and -64 mV, Cs+ increased the resting potential of all cells by between 2.5 and 21 mV. These results indicate that Ih contributes a tonic inward (depolarizing) component to the maintenance of the resting membrane potential in SCG neurones.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/s004240050653</identifier><identifier>PMID: 9644226</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Cells, Cultured ; Cyclic Nucleotide-Gated Cation Channels ; Depolarization ; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels ; Ion Channels - physiology ; Kinetics ; Membrane Potentials - physiology ; Nerve Tissue Proteins - physiology ; Neurons - physiology ; Patch-Clamp Techniques ; Potassium Channels ; Rats ; Superior Cervical Ganglion - cytology ; Superior Cervical Ganglion - physiology</subject><ispartof>Pflügers Archiv, 1998-08, Vol.436 (3), p.429-435</ispartof><rights>Springer-Verlag Berlin Heidelberg 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-c96741bbcee31e66b7ad9b26b7ba661022a59e436ef923307a3e656b0f44f7383</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9644226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lamas, J A</creatorcontrib><title>A hyperpolarization-activated cation current (Ih) contributes to resting membrane potential in rat superior cervical sympathetic neurones</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch</addtitle><description>Using perforated-patch voltage-clamp recording, a prominent hyperpolarization-activated inward cation current (Ih) has been identified in dissociated, cultured and replated, superior cervical sympathetic (SCG) neurones from 17-day-old rats. Ih was identified as a slowly activated inward current on hyperpolarizing from -60 mV, with an extrapolated null potential (in 3 mM [K+]out) of -42 mV. The activation range for Ih was -40 to -100 mV, with a half-activation voltage (V0.5) of -63 mV. The current was suppressed by 1 mM Cs+ but not by 1 mM Ba2+. The reversal potential for the current change induced by Cs+ agreed with the null potential for Ih. Ih conferred strong inward rectification to the current-voltage curve negative to -55 mV in both voltage-clamp and current-clamp recording. This inward rectification was reduced by 1 mM Cs+. In a sample of eight cells with initial resting membrane potentials between -51 and -64 mV, Cs+ increased the resting potential of all cells by between 2.5 and 21 mV. These results indicate that Ih contributes a tonic inward (depolarizing) component to the maintenance of the resting membrane potential in SCG neurones.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Cyclic Nucleotide-Gated Cation Channels</subject><subject>Depolarization</subject><subject>Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels</subject><subject>Ion Channels - physiology</subject><subject>Kinetics</subject><subject>Membrane Potentials - physiology</subject><subject>Nerve Tissue Proteins - physiology</subject><subject>Neurons - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channels</subject><subject>Rats</subject><subject>Superior Cervical Ganglion - cytology</subject><subject>Superior Cervical Ganglion - physiology</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp90U1rFTEUBuBQlHpbXboUgotSF6MnHzeZWZbSLyi40fWQ5J7pTZlJxiRTuP0H_dem7UXQhasDycPJG15CPjL4ygD0twwguQRYg1qLA7JiUvCGAxNvyApAsEZp1b4jRznfAwCXLT8kh52SknO1Ik9ndLubMc1xNMk_muJjaIwr_sEU3FD3ckDdkhKGQk9vtl-oi6Ekb5eCmZZIE-biwx2dcLLJBKRzLNV6M1IfaDKF5qU-4GOiDtODd_Ui76bZlC0W72jAJcWA-T15O5gx44f9PCY_Ly9-nF83t9-vbs7PbhsnZFsa1yktmbUOUTBUymqz6Syv0xqlGHBu1h1KoXDouBCgjUC1VhYGKQctWnFMTl73zin-Wmr4fvLZ4TjW7HHJve46zUCKCk__C5ngQnctb5_p53_ofVxSqN_oWy1rJq1YRc0rcinmnHDo5-Qnk3Y9g_65yv6vKqv_tF-62Ak3f_S-O_Eb6oGa6Q</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Lamas, J A</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>A hyperpolarization-activated cation current (Ih) contributes to resting membrane potential in rat superior cervical sympathetic neurones</title><author>Lamas, J A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-c96741bbcee31e66b7ad9b26b7ba661022a59e436ef923307a3e656b0f44f7383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Cyclic Nucleotide-Gated Cation Channels</topic><topic>Depolarization</topic><topic>Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels</topic><topic>Ion Channels - physiology</topic><topic>Kinetics</topic><topic>Membrane Potentials - physiology</topic><topic>Nerve Tissue Proteins - physiology</topic><topic>Neurons - physiology</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channels</topic><topic>Rats</topic><topic>Superior Cervical Ganglion - cytology</topic><topic>Superior Cervical Ganglion - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lamas, J A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lamas, J A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A hyperpolarization-activated cation current (Ih) contributes to resting membrane potential in rat superior cervical sympathetic neurones</atitle><jtitle>Pflügers Archiv</jtitle><addtitle>Pflugers Arch</addtitle><date>1998-08-01</date><risdate>1998</risdate><volume>436</volume><issue>3</issue><spage>429</spage><epage>435</epage><pages>429-435</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>Using perforated-patch voltage-clamp recording, a prominent hyperpolarization-activated inward cation current (Ih) has been identified in dissociated, cultured and replated, superior cervical sympathetic (SCG) neurones from 17-day-old rats. Ih was identified as a slowly activated inward current on hyperpolarizing from -60 mV, with an extrapolated null potential (in 3 mM [K+]out) of -42 mV. The activation range for Ih was -40 to -100 mV, with a half-activation voltage (V0.5) of -63 mV. The current was suppressed by 1 mM Cs+ but not by 1 mM Ba2+. The reversal potential for the current change induced by Cs+ agreed with the null potential for Ih. Ih conferred strong inward rectification to the current-voltage curve negative to -55 mV in both voltage-clamp and current-clamp recording. This inward rectification was reduced by 1 mM Cs+. In a sample of eight cells with initial resting membrane potentials between -51 and -64 mV, Cs+ increased the resting potential of all cells by between 2.5 and 21 mV. These results indicate that Ih contributes a tonic inward (depolarizing) component to the maintenance of the resting membrane potential in SCG neurones.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>9644226</pmid><doi>10.1007/s004240050653</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Cells, Cultured Cyclic Nucleotide-Gated Cation Channels Depolarization Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels Ion Channels - physiology Kinetics Membrane Potentials - physiology Nerve Tissue Proteins - physiology Neurons - physiology Patch-Clamp Techniques Potassium Channels Rats Superior Cervical Ganglion - cytology Superior Cervical Ganglion - physiology |
| title | A hyperpolarization-activated cation current (Ih) contributes to resting membrane potential in rat superior cervical sympathetic neurones |
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