Virus-like particles in the liver of a patient with fulminant hepatitis and antibody to hepatitis E virus
In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non‐A, non‐B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological...
Gespeichert in:
Veröffentlicht in: | Journal of medical virology 1990-07, Vol.31 (3), p.229-233 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 233 |
---|---|
container_issue | 3 |
container_start_page | 229 |
container_title | Journal of medical virology |
container_volume | 31 |
creator | Shavrina Asher, Ludmila V. Innis, Bruce L. Shrestha, Mrigendra P. Ticehurst, John Baze, Wallace B. |
description | In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non‐A, non‐B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the liver of a young pregnant woman from Nepal who died as a result of fulminant NANB hepatitis. IgM antibody to HEV was detected in the patient's serum by immune electron microscopy, suggesting that she was acutely infected with that virus. On light microscopic examination of the liver we observed cholestatic hepatitis with proliferation of bile ductules and pseudoglandular arrangement of hepatocytes around distended bile canaliculi. Three types of virus‐like particles were detected by electron microscopy. The most frequently observed particles were in cells lining small bile ductules; they measured 32–37 nm and were enclosed by a membrane. Particles of a second type were seen in clusters in the sinusoidal cells; they were uniform in size, without a membrane, and measured about 32 nm in diameter. Particles of a third type (65 nm) were found in epithelial cells of the small bile ductules. Among the particles we detected, the 32 nm particles most closely resembled those of HEV. |
doi_str_mv | 10.1002/jmv.1890310310 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79966869</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15884177</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4390-4e385216bed6591d3a6c10796c011755fd521ba492bf3a2cce5b34244a3ff2733</originalsourceid><addsrcrecordid>eNqFkUtvGyEUhVHVKHXSbrurxKbdjcOFAYZlZSVOo_SxaNPuEMMwMsk8XGCc-N8Hy1bSriKBEPrOORdxEHoPZA6E0LPbfjOHShEGu_UKzYAoUSgi4TWaEShFIQTwN-gkxltCSKUoPUbHlCngQGbI3_gwxaLzdw6vTUjedi5iP-C0crjzGxfw2GKTWfJuSPjepxVup673g8nXlduB5CM2Q5N38vXYbHEa_yHneLOb8RYdtaaL7t3hPEW_Ls5_Li6L6-_LL4vP14UtmSJF6VjFKYjaNYIraJgRFohUwhIAyXnbZFqbUtG6ZYZa63jNSlqWhrUtlYydok_73HUY_04uJt37aF3XmcGNU9RSKSEqoV4UAq-qEqTMwvleaMMYY3CtXgffm7DVQPSuBJ1L0M8lZMOHQ_JU9655kh9-PfOPB26iNV0bzGB9fE5VXBJZQdapve7ed277wlR99fXmvzcUe6-PyT08eU2400IyyfXvb0v9Y8Elvfiz1FfsEWEZryA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15884177</pqid></control><display><type>article</type><title>Virus-like particles in the liver of a patient with fulminant hepatitis and antibody to hepatitis E virus</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Shavrina Asher, Ludmila V. ; Innis, Bruce L. ; Shrestha, Mrigendra P. ; Ticehurst, John ; Baze, Wallace B.</creator><creatorcontrib>Shavrina Asher, Ludmila V. ; Innis, Bruce L. ; Shrestha, Mrigendra P. ; Ticehurst, John ; Baze, Wallace B.</creatorcontrib><description>In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non‐A, non‐B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the liver of a young pregnant woman from Nepal who died as a result of fulminant NANB hepatitis. IgM antibody to HEV was detected in the patient's serum by immune electron microscopy, suggesting that she was acutely infected with that virus. On light microscopic examination of the liver we observed cholestatic hepatitis with proliferation of bile ductules and pseudoglandular arrangement of hepatocytes around distended bile canaliculi. Three types of virus‐like particles were detected by electron microscopy. The most frequently observed particles were in cells lining small bile ductules; they measured 32–37 nm and were enclosed by a membrane. Particles of a second type were seen in clusters in the sinusoidal cells; they were uniform in size, without a membrane, and measured about 32 nm in diameter. Particles of a third type (65 nm) were found in epithelial cells of the small bile ductules. Among the particles we detected, the 32 nm particles most closely resembled those of HEV.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.1890310310</identifier><identifier>PMID: 2391510</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Antibodies, Viral - blood ; Biological and medical sciences ; electron microscopy ; ENANB hepatitis ; Female ; Hepatitis Viruses - immunology ; Hepatitis Viruses - ultrastructure ; Hepatitis, Viral, Human - complications ; Hepatitis, Viral, Human - microbiology ; Hepatitis, Viral, Human - pathology ; HEV ; Human viral diseases ; Humans ; Immunoglobulin M - metabolism ; Inclusion Bodies, Viral - ultrastructure ; Infectious diseases ; Liver - microbiology ; Liver - ultrastructure ; Medical sciences ; Microscopy, Electron ; Pregnancy ; Pregnancy Complications, Infectious - immunology ; Pregnancy Complications, Infectious - microbiology ; Pregnancy Complications, Infectious - pathology ; Viral diseases ; Viral hepatitis</subject><ispartof>Journal of medical virology, 1990-07, Vol.31 (3), p.229-233</ispartof><rights>Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4390-4e385216bed6591d3a6c10796c011755fd521ba492bf3a2cce5b34244a3ff2733</citedby><cites>FETCH-LOGICAL-c4390-4e385216bed6591d3a6c10796c011755fd521ba492bf3a2cce5b34244a3ff2733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.1890310310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.1890310310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19570781$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2391510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shavrina Asher, Ludmila V.</creatorcontrib><creatorcontrib>Innis, Bruce L.</creatorcontrib><creatorcontrib>Shrestha, Mrigendra P.</creatorcontrib><creatorcontrib>Ticehurst, John</creatorcontrib><creatorcontrib>Baze, Wallace B.</creatorcontrib><title>Virus-like particles in the liver of a patient with fulminant hepatitis and antibody to hepatitis E virus</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non‐A, non‐B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the liver of a young pregnant woman from Nepal who died as a result of fulminant NANB hepatitis. IgM antibody to HEV was detected in the patient's serum by immune electron microscopy, suggesting that she was acutely infected with that virus. On light microscopic examination of the liver we observed cholestatic hepatitis with proliferation of bile ductules and pseudoglandular arrangement of hepatocytes around distended bile canaliculi. Three types of virus‐like particles were detected by electron microscopy. The most frequently observed particles were in cells lining small bile ductules; they measured 32–37 nm and were enclosed by a membrane. Particles of a second type were seen in clusters in the sinusoidal cells; they were uniform in size, without a membrane, and measured about 32 nm in diameter. Particles of a third type (65 nm) were found in epithelial cells of the small bile ductules. Among the particles we detected, the 32 nm particles most closely resembled those of HEV.</description><subject>Adult</subject><subject>Antibodies, Viral - blood</subject><subject>Biological and medical sciences</subject><subject>electron microscopy</subject><subject>ENANB hepatitis</subject><subject>Female</subject><subject>Hepatitis Viruses - immunology</subject><subject>Hepatitis Viruses - ultrastructure</subject><subject>Hepatitis, Viral, Human - complications</subject><subject>Hepatitis, Viral, Human - microbiology</subject><subject>Hepatitis, Viral, Human - pathology</subject><subject>HEV</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunoglobulin M - metabolism</subject><subject>Inclusion Bodies, Viral - ultrastructure</subject><subject>Infectious diseases</subject><subject>Liver - microbiology</subject><subject>Liver - ultrastructure</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Infectious - immunology</subject><subject>Pregnancy Complications, Infectious - microbiology</subject><subject>Pregnancy Complications, Infectious - pathology</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvGyEUhVHVKHXSbrurxKbdjcOFAYZlZSVOo_SxaNPuEMMwMsk8XGCc-N8Hy1bSriKBEPrOORdxEHoPZA6E0LPbfjOHShEGu_UKzYAoUSgi4TWaEShFIQTwN-gkxltCSKUoPUbHlCngQGbI3_gwxaLzdw6vTUjedi5iP-C0crjzGxfw2GKTWfJuSPjepxVup673g8nXlduB5CM2Q5N38vXYbHEa_yHneLOb8RYdtaaL7t3hPEW_Ls5_Li6L6-_LL4vP14UtmSJF6VjFKYjaNYIraJgRFohUwhIAyXnbZFqbUtG6ZYZa63jNSlqWhrUtlYydok_73HUY_04uJt37aF3XmcGNU9RSKSEqoV4UAq-qEqTMwvleaMMYY3CtXgffm7DVQPSuBJ1L0M8lZMOHQ_JU9655kh9-PfOPB26iNV0bzGB9fE5VXBJZQdapve7ed277wlR99fXmvzcUe6-PyT08eU2400IyyfXvb0v9Y8Elvfiz1FfsEWEZryA</recordid><startdate>199007</startdate><enddate>199007</enddate><creator>Shavrina Asher, Ludmila V.</creator><creator>Innis, Bruce L.</creator><creator>Shrestha, Mrigendra P.</creator><creator>Ticehurst, John</creator><creator>Baze, Wallace B.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199007</creationdate><title>Virus-like particles in the liver of a patient with fulminant hepatitis and antibody to hepatitis E virus</title><author>Shavrina Asher, Ludmila V. ; Innis, Bruce L. ; Shrestha, Mrigendra P. ; Ticehurst, John ; Baze, Wallace B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4390-4e385216bed6591d3a6c10796c011755fd521ba492bf3a2cce5b34244a3ff2733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adult</topic><topic>Antibodies, Viral - blood</topic><topic>Biological and medical sciences</topic><topic>electron microscopy</topic><topic>ENANB hepatitis</topic><topic>Female</topic><topic>Hepatitis Viruses - immunology</topic><topic>Hepatitis Viruses - ultrastructure</topic><topic>Hepatitis, Viral, Human - complications</topic><topic>Hepatitis, Viral, Human - microbiology</topic><topic>Hepatitis, Viral, Human - pathology</topic><topic>HEV</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunoglobulin M - metabolism</topic><topic>Inclusion Bodies, Viral - ultrastructure</topic><topic>Infectious diseases</topic><topic>Liver - microbiology</topic><topic>Liver - ultrastructure</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Infectious - immunology</topic><topic>Pregnancy Complications, Infectious - microbiology</topic><topic>Pregnancy Complications, Infectious - pathology</topic><topic>Viral diseases</topic><topic>Viral hepatitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shavrina Asher, Ludmila V.</creatorcontrib><creatorcontrib>Innis, Bruce L.</creatorcontrib><creatorcontrib>Shrestha, Mrigendra P.</creatorcontrib><creatorcontrib>Ticehurst, John</creatorcontrib><creatorcontrib>Baze, Wallace B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shavrina Asher, Ludmila V.</au><au>Innis, Bruce L.</au><au>Shrestha, Mrigendra P.</au><au>Ticehurst, John</au><au>Baze, Wallace B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virus-like particles in the liver of a patient with fulminant hepatitis and antibody to hepatitis E virus</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>1990-07</date><risdate>1990</risdate><volume>31</volume><issue>3</issue><spage>229</spage><epage>233</epage><pages>229-233</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non‐A, non‐B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the liver of a young pregnant woman from Nepal who died as a result of fulminant NANB hepatitis. IgM antibody to HEV was detected in the patient's serum by immune electron microscopy, suggesting that she was acutely infected with that virus. On light microscopic examination of the liver we observed cholestatic hepatitis with proliferation of bile ductules and pseudoglandular arrangement of hepatocytes around distended bile canaliculi. Three types of virus‐like particles were detected by electron microscopy. The most frequently observed particles were in cells lining small bile ductules; they measured 32–37 nm and were enclosed by a membrane. Particles of a second type were seen in clusters in the sinusoidal cells; they were uniform in size, without a membrane, and measured about 32 nm in diameter. Particles of a third type (65 nm) were found in epithelial cells of the small bile ductules. Among the particles we detected, the 32 nm particles most closely resembled those of HEV.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2391510</pmid><doi>10.1002/jmv.1890310310</doi><tpages>5</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0146-6615 |
ispartof | Journal of medical virology, 1990-07, Vol.31 (3), p.229-233 |
issn | 0146-6615 1096-9071 |
language | eng |
recordid | cdi_proquest_miscellaneous_79966869 |
source | MEDLINE; Access via Wiley Online Library |
subjects | Adult Antibodies, Viral - blood Biological and medical sciences electron microscopy ENANB hepatitis Female Hepatitis Viruses - immunology Hepatitis Viruses - ultrastructure Hepatitis, Viral, Human - complications Hepatitis, Viral, Human - microbiology Hepatitis, Viral, Human - pathology HEV Human viral diseases Humans Immunoglobulin M - metabolism Inclusion Bodies, Viral - ultrastructure Infectious diseases Liver - microbiology Liver - ultrastructure Medical sciences Microscopy, Electron Pregnancy Pregnancy Complications, Infectious - immunology Pregnancy Complications, Infectious - microbiology Pregnancy Complications, Infectious - pathology Viral diseases Viral hepatitis |
title | Virus-like particles in the liver of a patient with fulminant hepatitis and antibody to hepatitis E virus |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T22%3A24%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Virus-like%20particles%20in%20the%20liver%20of%20a%20patient%20with%20fulminant%20hepatitis%20and%20antibody%20to%20hepatitis%20E%20virus&rft.jtitle=Journal%20of%20medical%20virology&rft.au=Shavrina%20Asher,%20Ludmila%20V.&rft.date=1990-07&rft.volume=31&rft.issue=3&rft.spage=229&rft.epage=233&rft.pages=229-233&rft.issn=0146-6615&rft.eissn=1096-9071&rft.coden=JMVIDB&rft_id=info:doi/10.1002/jmv.1890310310&rft_dat=%3Cproquest_cross%3E15884177%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15884177&rft_id=info:pmid/2391510&rfr_iscdi=true |