Flow cytometric measurement of neutrophil alkaline phosphatase before and during initiation of an induced Escherichia coli mastitis in cattle
In 12 healthy cows, neutrophil alkaline phosphatase (NAP) activity was measured by flow cytometer before and during an experimentally induced Escherichia coli mastitis, to study the role and increase of NAP in Gram-negative bacterial infections. Percentage of neutrophils containing alkaline phosphat...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 1998-04, Vol.62 (3), p.235-244 |
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| creator | van Werven, Tine Piens, Kathleen van den Broek, Jan Heyneman, Rogier Noordhuizen-Stassen, Elsbeth N Schukken, Ynte H Brand, Arie |
| description | In 12 healthy cows, neutrophil alkaline phosphatase (NAP) activity was measured by flow cytometer before and during an experimentally induced
Escherichia coli mastitis, to study the role and increase of NAP in Gram-negative bacterial infections. Percentage of neutrophils containing alkaline phosphatase and intensity of NAP activity were measured. Preinfection percentage of neutrophils with enzyme activity varied between 64.0% and 84.4% and the intensity of enzyme activity was low in all cows. After induction of infection, percentage of neutrophils with enzyme activity showed a significant decrease on day 1 followed by an significant increase on day 3. NAP intensity increased significantly on the second and third day after infection. This increase of intensity was significantly, positively correlated with the severity of infection. From this study we may conclude that variation in susceptibility to
E. coli mastitis could not be explained by preinfection NAP levels. The post-infection increase of NAP activity, that was found following an induced infection was more a result of increased enzyme intensity per neutrophil, then from an increase of percentage neutrophils with enzyme activity. Furthermore, a strong correlation was found between NAP intensity and severity of inflammation. There was evidence that the more severely diseased animals showed stronger NAP intensity increase. |
| doi_str_mv | 10.1016/S0165-2427(97)00168-2 |
| format | Article |
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Escherichia coli mastitis, to study the role and increase of NAP in Gram-negative bacterial infections. Percentage of neutrophils containing alkaline phosphatase and intensity of NAP activity were measured. Preinfection percentage of neutrophils with enzyme activity varied between 64.0% and 84.4% and the intensity of enzyme activity was low in all cows. After induction of infection, percentage of neutrophils with enzyme activity showed a significant decrease on day 1 followed by an significant increase on day 3. NAP intensity increased significantly on the second and third day after infection. This increase of intensity was significantly, positively correlated with the severity of infection. From this study we may conclude that variation in susceptibility to
E. coli mastitis could not be explained by preinfection NAP levels. The post-infection increase of NAP activity, that was found following an induced infection was more a result of increased enzyme intensity per neutrophil, then from an increase of percentage neutrophils with enzyme activity. Furthermore, a strong correlation was found between NAP intensity and severity of inflammation. There was evidence that the more severely diseased animals showed stronger NAP intensity increase.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/S0165-2427(97)00168-2</identifier><identifier>PMID: 9643457</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ALKALINE PHOSPHATASE ; Alkaline Phosphatase - metabolism ; Animals ; BOVIN ; BOVINE MASTITIS ; CATTLE ; Colony Count, Microbial - veterinary ; Enzyme activity ; ENZYMIC ACTIVITY ; ESCHERICHIA COLI ; Escherichia coli Infections - enzymology ; Escherichia coli Infections - veterinary ; Female ; Flow cytometry ; Flow Cytometry - veterinary ; FOSFATASA ALCALINA ; GANADO BOVINO ; GRANULOCITOS ; GRANULOCYTE ; GRANULOCYTES ; Infection ; MAMMITE BOVINE ; Mastitis ; MASTITIS BOVINA ; Mastitis, Bovine - enzymology ; Mastitis, Bovine - microbiology ; Milk - microbiology ; Neutrophils - enzymology ; PHOSPHATASE ALCALINE</subject><ispartof>Veterinary immunology and immunopathology, 1998-04, Vol.62 (3), p.235-244</ispartof><rights>1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-e0c81418c14d88e54f8dfd401521339bdc477857be99f90d08d23ab2b009a4973</citedby><cites>FETCH-LOGICAL-c413t-e0c81418c14d88e54f8dfd401521339bdc477857be99f90d08d23ab2b009a4973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0165-2427(97)00168-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9643457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Werven, Tine</creatorcontrib><creatorcontrib>Piens, Kathleen</creatorcontrib><creatorcontrib>van den Broek, Jan</creatorcontrib><creatorcontrib>Heyneman, Rogier</creatorcontrib><creatorcontrib>Noordhuizen-Stassen, Elsbeth N</creatorcontrib><creatorcontrib>Schukken, Ynte H</creatorcontrib><creatorcontrib>Brand, Arie</creatorcontrib><title>Flow cytometric measurement of neutrophil alkaline phosphatase before and during initiation of an induced Escherichia coli mastitis in cattle</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>In 12 healthy cows, neutrophil alkaline phosphatase (NAP) activity was measured by flow cytometer before and during an experimentally induced
Escherichia coli mastitis, to study the role and increase of NAP in Gram-negative bacterial infections. Percentage of neutrophils containing alkaline phosphatase and intensity of NAP activity were measured. Preinfection percentage of neutrophils with enzyme activity varied between 64.0% and 84.4% and the intensity of enzyme activity was low in all cows. After induction of infection, percentage of neutrophils with enzyme activity showed a significant decrease on day 1 followed by an significant increase on day 3. NAP intensity increased significantly on the second and third day after infection. This increase of intensity was significantly, positively correlated with the severity of infection. From this study we may conclude that variation in susceptibility to
E. coli mastitis could not be explained by preinfection NAP levels. The post-infection increase of NAP activity, that was found following an induced infection was more a result of increased enzyme intensity per neutrophil, then from an increase of percentage neutrophils with enzyme activity. Furthermore, a strong correlation was found between NAP intensity and severity of inflammation. There was evidence that the more severely diseased animals showed stronger NAP intensity increase.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ALKALINE PHOSPHATASE</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>BOVIN</subject><subject>BOVINE MASTITIS</subject><subject>CATTLE</subject><subject>Colony Count, Microbial - veterinary</subject><subject>Enzyme activity</subject><subject>ENZYMIC ACTIVITY</subject><subject>ESCHERICHIA COLI</subject><subject>Escherichia coli Infections - enzymology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Flow Cytometry - veterinary</subject><subject>FOSFATASA ALCALINA</subject><subject>GANADO BOVINO</subject><subject>GRANULOCITOS</subject><subject>GRANULOCYTE</subject><subject>GRANULOCYTES</subject><subject>Infection</subject><subject>MAMMITE BOVINE</subject><subject>Mastitis</subject><subject>MASTITIS BOVINA</subject><subject>Mastitis, Bovine - enzymology</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Milk - microbiology</subject><subject>Neutrophils - enzymology</subject><subject>PHOSPHATASE ALCALINE</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFuFCEUhonR1LX6CE24MnoxCgwscGVM06rJRi_Ua8LAmQ46Ayswmj6E7yzb3dTLJgQC5_vPT86P0AUlbyih27df2yY6xpl8peVr0m6qY4_QhirZd0z0_DHa3CNP0bNSfhBChFbqDJ3pLe-5kBv093pOf7C7rWmBmoPDC9iyZlggVpxGHGGtOe2nMGM7_7RziID3Uyr7yVZbAA8wpgzYRo_9mkO8wSGGGmwNKR70NrYHvzrw-Kq4CZrFFCx2aQ54saU2tjQCO1vrDM_Rk9HOBV6cznP0_frq2-XHbvflw6fL97vOcdrXDohTlFPlKPdKgeCj8qPnhApG-14P3nEplZADaD1q4onyrLcDGwjRlmvZn6OXx777nH6tUKpZQnEwzzZCWouRWm8FU_pBkG570RZtoDiCLqdSMoxmn8Ni862hxBzyMnd5mUMYRktzl5dhTXdxMliHBfy96hTQ__pok7E3ORTzeUe1VoQwQQ6-7451aOP6HSCb4gLENu-QwVXjU3jgB_8Af96wNA</recordid><startdate>19980416</startdate><enddate>19980416</enddate><creator>van Werven, Tine</creator><creator>Piens, Kathleen</creator><creator>van den Broek, Jan</creator><creator>Heyneman, Rogier</creator><creator>Noordhuizen-Stassen, Elsbeth N</creator><creator>Schukken, Ynte H</creator><creator>Brand, Arie</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980416</creationdate><title>Flow cytometric measurement of neutrophil alkaline phosphatase before and during initiation of an induced Escherichia coli mastitis in cattle</title><author>van Werven, Tine ; Piens, Kathleen ; van den Broek, Jan ; Heyneman, Rogier ; Noordhuizen-Stassen, Elsbeth N ; Schukken, Ynte H ; Brand, Arie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-e0c81418c14d88e54f8dfd401521339bdc477857be99f90d08d23ab2b009a4973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ALKALINE PHOSPHATASE</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>BOVIN</topic><topic>BOVINE MASTITIS</topic><topic>CATTLE</topic><topic>Colony Count, Microbial - veterinary</topic><topic>Enzyme activity</topic><topic>ENZYMIC ACTIVITY</topic><topic>ESCHERICHIA COLI</topic><topic>Escherichia coli Infections - enzymology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Flow Cytometry - veterinary</topic><topic>FOSFATASA ALCALINA</topic><topic>GANADO BOVINO</topic><topic>GRANULOCITOS</topic><topic>GRANULOCYTE</topic><topic>GRANULOCYTES</topic><topic>Infection</topic><topic>MAMMITE BOVINE</topic><topic>Mastitis</topic><topic>MASTITIS BOVINA</topic><topic>Mastitis, Bovine - enzymology</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Milk - microbiology</topic><topic>Neutrophils - enzymology</topic><topic>PHOSPHATASE ALCALINE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Werven, Tine</creatorcontrib><creatorcontrib>Piens, Kathleen</creatorcontrib><creatorcontrib>van den Broek, Jan</creatorcontrib><creatorcontrib>Heyneman, Rogier</creatorcontrib><creatorcontrib>Noordhuizen-Stassen, Elsbeth N</creatorcontrib><creatorcontrib>Schukken, Ynte H</creatorcontrib><creatorcontrib>Brand, Arie</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Werven, Tine</au><au>Piens, Kathleen</au><au>van den Broek, Jan</au><au>Heyneman, Rogier</au><au>Noordhuizen-Stassen, Elsbeth N</au><au>Schukken, Ynte H</au><au>Brand, Arie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flow cytometric measurement of neutrophil alkaline phosphatase before and during initiation of an induced Escherichia coli mastitis in cattle</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>1998-04-16</date><risdate>1998</risdate><volume>62</volume><issue>3</issue><spage>235</spage><epage>244</epage><pages>235-244</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>In 12 healthy cows, neutrophil alkaline phosphatase (NAP) activity was measured by flow cytometer before and during an experimentally induced
Escherichia coli mastitis, to study the role and increase of NAP in Gram-negative bacterial infections. Percentage of neutrophils containing alkaline phosphatase and intensity of NAP activity were measured. Preinfection percentage of neutrophils with enzyme activity varied between 64.0% and 84.4% and the intensity of enzyme activity was low in all cows. After induction of infection, percentage of neutrophils with enzyme activity showed a significant decrease on day 1 followed by an significant increase on day 3. NAP intensity increased significantly on the second and third day after infection. This increase of intensity was significantly, positively correlated with the severity of infection. From this study we may conclude that variation in susceptibility to
E. coli mastitis could not be explained by preinfection NAP levels. The post-infection increase of NAP activity, that was found following an induced infection was more a result of increased enzyme intensity per neutrophil, then from an increase of percentage neutrophils with enzyme activity. Furthermore, a strong correlation was found between NAP intensity and severity of inflammation. There was evidence that the more severely diseased animals showed stronger NAP intensity increase.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9643457</pmid><doi>10.1016/S0165-2427(97)00168-2</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 1998-04, Vol.62 (3), p.235-244 |
| issn | 0165-2427 1873-2534 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79965289 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ALKALINE PHOSPHATASE Alkaline Phosphatase - metabolism Animals BOVIN BOVINE MASTITIS CATTLE Colony Count, Microbial - veterinary Enzyme activity ENZYMIC ACTIVITY ESCHERICHIA COLI Escherichia coli Infections - enzymology Escherichia coli Infections - veterinary Female Flow cytometry Flow Cytometry - veterinary FOSFATASA ALCALINA GANADO BOVINO GRANULOCITOS GRANULOCYTE GRANULOCYTES Infection MAMMITE BOVINE Mastitis MASTITIS BOVINA Mastitis, Bovine - enzymology Mastitis, Bovine - microbiology Milk - microbiology Neutrophils - enzymology PHOSPHATASE ALCALINE |
| title | Flow cytometric measurement of neutrophil alkaline phosphatase before and during initiation of an induced Escherichia coli mastitis in cattle |
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