Inhibition of cytochrome oxidase by dibucaine
Dibucaine-HCl inhibited mitochondrial cytochrome c oxidase activity in intact mitochondria with 50% inhibition occurring at 1.1 mM dibucaine-HCl. Dibucaine-HCl did not prevent the reduction of cytochrome oxidase by ascorbate plus N, N, N′, N′-tetramethyl- p-phenylenediamine dihydrochloride (TMPD) wh...
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| Veröffentlicht in: | Biochemical pharmacology 1990-09, Vol.40 (5), p.1077-1081 |
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| container_title | Biochemical pharmacology |
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| creator | Stringer, Bradley K. Harmon, H.James |
| description | Dibucaine-HCl inhibited mitochondrial cytochrome
c oxidase activity in intact mitochondria with 50% inhibition occurring at 1.1 mM dibucaine-HCl. Dibucaine-HCl did not prevent the reduction of cytochrome oxidase by ascorbate plus
N,
N,
N′,
N′-tetramethyl-
p-phenylenediamine dihydrochloride (TMPD) when measured at 604 nm but prevented 50% of the absorbance change at 445 nm; dithionite reduced the oxidase completely. Dibucaine prevented binding of CO to oxidase reduced with ascorbate plus TMPD by preventing the reduction of cytochrome
a
3. The midpotentials of cytochrome
c and cytochrome oxidase, the visible absorbance wavelength maxima, and the position and intensity of the signals of the EPR spectrum of the oxidase were not affected. Dibucaine-HCl prevented ascorbate plus TMPD-driven reduction of the near infra-red detectable copper center associated with cytochrome
a; dithionite subsequently reduced this center. Dibucaine-HCl inhibited cytochrome oxidase activity by interacting between cytochrome
a and its associated copper. Since respiration was 8-fold less sensitive in submitochondrial particles, this site of inhibition is on the cytoplasmic side of the membrane. |
| doi_str_mv | 10.1016/0006-2952(90)90496-8 |
| format | Article |
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c oxidase activity in intact mitochondria with 50% inhibition occurring at 1.1 mM dibucaine-HCl. Dibucaine-HCl did not prevent the reduction of cytochrome oxidase by ascorbate plus
N,
N,
N′,
N′-tetramethyl-
p-phenylenediamine dihydrochloride (TMPD) when measured at 604 nm but prevented 50% of the absorbance change at 445 nm; dithionite reduced the oxidase completely. Dibucaine prevented binding of CO to oxidase reduced with ascorbate plus TMPD by preventing the reduction of cytochrome
a
3. The midpotentials of cytochrome
c and cytochrome oxidase, the visible absorbance wavelength maxima, and the position and intensity of the signals of the EPR spectrum of the oxidase were not affected. Dibucaine-HCl prevented ascorbate plus TMPD-driven reduction of the near infra-red detectable copper center associated with cytochrome
a; dithionite subsequently reduced this center. Dibucaine-HCl inhibited cytochrome oxidase activity by interacting between cytochrome
a and its associated copper. Since respiration was 8-fold less sensitive in submitochondrial particles, this site of inhibition is on the cytoplasmic side of the membrane.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/0006-2952(90)90496-8</identifier><identifier>PMID: 2167679</identifier><identifier>CODEN: BCPCA6</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Anesthetics. Neuromuscular blocking agents ; Animals ; Biological and medical sciences ; Cattle ; dibucaine ; Dibucaine - pharmacology ; Electron Transport Complex IV - antagonists & inhibitors ; heart ; Medical sciences ; mitochondria ; Mitochondria, Heart - drug effects ; Mitochondria, Heart - enzymology ; Neuropharmacology ; Oxidation-Reduction ; Oxidoreductases - metabolism ; Pharmacology. Drug treatments ; Tetramethylphenylenediamine - pharmacology</subject><ispartof>Biochemical pharmacology, 1990-09, Vol.40 (5), p.1077-1081</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-d8536d8e41f332711f6836b417efeea6a2f7a5af6f4915db2303b34a6f4fff463</citedby><cites>FETCH-LOGICAL-c418t-d8536d8e41f332711f6836b417efeea6a2f7a5af6f4915db2303b34a6f4fff463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-2952(90)90496-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19393234$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2167679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stringer, Bradley K.</creatorcontrib><creatorcontrib>Harmon, H.James</creatorcontrib><title>Inhibition of cytochrome oxidase by dibucaine</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>Dibucaine-HCl inhibited mitochondrial cytochrome
c oxidase activity in intact mitochondria with 50% inhibition occurring at 1.1 mM dibucaine-HCl. Dibucaine-HCl did not prevent the reduction of cytochrome oxidase by ascorbate plus
N,
N,
N′,
N′-tetramethyl-
p-phenylenediamine dihydrochloride (TMPD) when measured at 604 nm but prevented 50% of the absorbance change at 445 nm; dithionite reduced the oxidase completely. Dibucaine prevented binding of CO to oxidase reduced with ascorbate plus TMPD by preventing the reduction of cytochrome
a
3. The midpotentials of cytochrome
c and cytochrome oxidase, the visible absorbance wavelength maxima, and the position and intensity of the signals of the EPR spectrum of the oxidase were not affected. Dibucaine-HCl prevented ascorbate plus TMPD-driven reduction of the near infra-red detectable copper center associated with cytochrome
a; dithionite subsequently reduced this center. Dibucaine-HCl inhibited cytochrome oxidase activity by interacting between cytochrome
a and its associated copper. Since respiration was 8-fold less sensitive in submitochondrial particles, this site of inhibition is on the cytoplasmic side of the membrane.</description><subject>Anesthetics. Neuromuscular blocking agents</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>dibucaine</subject><subject>Dibucaine - pharmacology</subject><subject>Electron Transport Complex IV - antagonists & inhibitors</subject><subject>heart</subject><subject>Medical sciences</subject><subject>mitochondria</subject><subject>Mitochondria, Heart - drug effects</subject><subject>Mitochondria, Heart - enzymology</subject><subject>Neuropharmacology</subject><subject>Oxidation-Reduction</subject><subject>Oxidoreductases - metabolism</subject><subject>Pharmacology. Drug treatments</subject><subject>Tetramethylphenylenediamine - pharmacology</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LwzAYgIMoc07_gUIvih6qSZOmyUWQ4cdg4EXPIU3fsEjbaNKJ-_e2rsybnsKb98lDeBA6JfiaYMJvMMY8zWSeXUp8JTGTPBV7aEpEQftrLvbRdIccoqMY34ZRcDJBk4zwghdyitJFu3Kl65xvE28Ts-m8WQXfQOK_XKUjJOUmqVy5Ntq1cIwOrK4jnIznDL0-3L_Mn9Ll8-NifrdMDSOiSyuRU14JYMRSmhWEWC4oLxkpwAJorjNb6FxbbpkkeVVmFNOSMt3P1lrG6QxdbL3vwX-sIXaqcdFAXesW_DqqQkqeMSz-BUlekJzwwci2oAk-xgBWvQfX6LBRBKshpxriqKGVklj95FSD_2z0r8sGqt2jsV-_Px_3Ohpd26Bb4-KvW1JJM8p67nbLQV_t00FQ0ThoDVQugOlU5d3fH_kGd42PjQ</recordid><startdate>19900901</startdate><enddate>19900901</enddate><creator>Stringer, Bradley K.</creator><creator>Harmon, H.James</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900901</creationdate><title>Inhibition of cytochrome oxidase by dibucaine</title><author>Stringer, Bradley K. ; Harmon, H.James</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-d8536d8e41f332711f6836b417efeea6a2f7a5af6f4915db2303b34a6f4fff463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Anesthetics. Neuromuscular blocking agents</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>dibucaine</topic><topic>Dibucaine - pharmacology</topic><topic>Electron Transport Complex IV - antagonists & inhibitors</topic><topic>heart</topic><topic>Medical sciences</topic><topic>mitochondria</topic><topic>Mitochondria, Heart - drug effects</topic><topic>Mitochondria, Heart - enzymology</topic><topic>Neuropharmacology</topic><topic>Oxidation-Reduction</topic><topic>Oxidoreductases - metabolism</topic><topic>Pharmacology. Drug treatments</topic><topic>Tetramethylphenylenediamine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stringer, Bradley K.</creatorcontrib><creatorcontrib>Harmon, H.James</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stringer, Bradley K.</au><au>Harmon, H.James</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of cytochrome oxidase by dibucaine</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1990-09-01</date><risdate>1990</risdate><volume>40</volume><issue>5</issue><spage>1077</spage><epage>1081</epage><pages>1077-1081</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><coden>BCPCA6</coden><abstract>Dibucaine-HCl inhibited mitochondrial cytochrome
c oxidase activity in intact mitochondria with 50% inhibition occurring at 1.1 mM dibucaine-HCl. Dibucaine-HCl did not prevent the reduction of cytochrome oxidase by ascorbate plus
N,
N,
N′,
N′-tetramethyl-
p-phenylenediamine dihydrochloride (TMPD) when measured at 604 nm but prevented 50% of the absorbance change at 445 nm; dithionite reduced the oxidase completely. Dibucaine prevented binding of CO to oxidase reduced with ascorbate plus TMPD by preventing the reduction of cytochrome
a
3. The midpotentials of cytochrome
c and cytochrome oxidase, the visible absorbance wavelength maxima, and the position and intensity of the signals of the EPR spectrum of the oxidase were not affected. Dibucaine-HCl prevented ascorbate plus TMPD-driven reduction of the near infra-red detectable copper center associated with cytochrome
a; dithionite subsequently reduced this center. Dibucaine-HCl inhibited cytochrome oxidase activity by interacting between cytochrome
a and its associated copper. Since respiration was 8-fold less sensitive in submitochondrial particles, this site of inhibition is on the cytoplasmic side of the membrane.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2167679</pmid><doi>10.1016/0006-2952(90)90496-8</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical pharmacology, 1990-09, Vol.40 (5), p.1077-1081 |
| issn | 0006-2952 1873-2968 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Anesthetics. Neuromuscular blocking agents Animals Biological and medical sciences Cattle dibucaine Dibucaine - pharmacology Electron Transport Complex IV - antagonists & inhibitors heart Medical sciences mitochondria Mitochondria, Heart - drug effects Mitochondria, Heart - enzymology Neuropharmacology Oxidation-Reduction Oxidoreductases - metabolism Pharmacology. Drug treatments Tetramethylphenylenediamine - pharmacology |
| title | Inhibition of cytochrome oxidase by dibucaine |
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