Interaction of insulin-like growth factors (IGF) with isolated sheep hepatocytes. I. Binding and degradation of IGF-I

The binding and degradation of 125I-hIGF-I by isolated sheep hepatocytes have been examined. Hepatocytes were isolated by collagenase perfusion of 32–55 kg wether lambs and were incubated at 20 or 37C at pH 7.4 in a 95% O 2/5% CO 2 atmosphere. Maximal binding was obtained at 60 min and declined slig...

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