Electrophoretic Characterization of Exposed Outer Membrane Proteins in Environmental and Human Bacteroides fragilis Strains
Bacteroides fragilis isolated from aquatic environment, from infectious processs and from human feces were compared as to their outer membrane protein electrophoretic profiles after staining with Coomassie blue and reacting with antibodies prepared against whole-cell antigens of a reference strain f...
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| Veröffentlicht in: | Zentralblatt für Bakteriologie 1998-05, Vol.287 (4), p.331-341 |
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| container_title | Zentralblatt für Bakteriologie |
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| creator | Domingues, Regina Maria Cavalcanti Pilotto Avelar, Kátia Eliane Santos Silva E Souza, Wilenes das Graças Moraes, Saulo Roni Antunes, Eduardo Nazarene France Ferreira, Maria Candida de Souza |
| description | Bacteroides fragilis isolated from aquatic environment, from infectious processs and from human feces were compared as to their outer membrane protein electrophoretic profiles after staining with Coomassie blue and reacting with antibodies prepared against whole-cell antigens of a reference strain from a clinical source. A marked homogeneity was found among the strains with these methodologies. The profiles of all strains obtained after radio-iodination of the intact cell showed qualitative similarity when compared with the profiles obtained by the other methods. Thus, these data allow us to suggest the designation of the peptides observed in the autoradiograms as surface-exposed proteins. Differences observed in the autoradiograms in the expression of bands mainly detected at a molecular weight of 28 in the commensal strain 118310 defined previously as avirulent, in addition to a distinction in the titres of agglutination with the sera tested and lower reactivity in the immunoblotting assays, suggest a relationship of the
B. fragilis surface architecture with the virulence potential as well as with the origin of the strain. |
| doi_str_mv | 10.1016/S0934-8840(98)80167-9 |
| format | Article |
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B. fragilis surface architecture with the virulence potential as well as with the origin of the strain.</description><subject>Agglutination Tests</subject><subject>Animals</subject><subject>Bacterial Outer Membrane Proteins - analysis</subject><subject>Bacteriology</subject><subject>Bacteroides fragilis</subject><subject>Bacteroides fragilis - chemistry</subject><subject>Bacteroides fragilis - isolation & purification</subject><subject>Bacteroides Infections - microbiology</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. 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A marked homogeneity was found among the strains with these methodologies. The profiles of all strains obtained after radio-iodination of the intact cell showed qualitative similarity when compared with the profiles obtained by the other methods. Thus, these data allow us to suggest the designation of the peptides observed in the autoradiograms as surface-exposed proteins. Differences observed in the autoradiograms in the expression of bands mainly detected at a molecular weight of 28 in the commensal strain 118310 defined previously as avirulent, in addition to a distinction in the titres of agglutination with the sera tested and lower reactivity in the immunoblotting assays, suggest a relationship of the
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| ispartof | Zentralblatt für Bakteriologie, 1998-05, Vol.287 (4), p.331-341 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Agglutination Tests Animals Bacterial Outer Membrane Proteins - analysis Bacteriology Bacteroides fragilis Bacteroides fragilis - chemistry Bacteroides fragilis - isolation & purification Bacteroides Infections - microbiology Biological and medical sciences Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Humans Immunoblotting Microbiology Morphology, structure, chemical composition Rabbits |
| title | Electrophoretic Characterization of Exposed Outer Membrane Proteins in Environmental and Human Bacteroides fragilis Strains |
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