Mapping sequences required for productive replication of beet necrotic yellow vein virus RNA 3
Of the four genome components of beet necrotic yellow vein virus only RNAs 1 and 2 are essential for viral replication in leaves. We have mapped cis-regulatory elements on RNA 3 by introducing deletions into expressible cDNA clones and inoculating leaves with the altered transcripts along with RNAs...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1990-09, Vol.178 (1), p.273-280 |
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| creator | Jupin, I. Richards, K. Jonard, G. Guilley, H. Pleij, C.W.A. |
| description | Of the four genome components of beet necrotic yellow vein virus only RNAs 1 and 2 are essential for viral replication in leaves. We have mapped cis-regulatory elements on RNA 3 by introducing deletions into expressible cDNA clones and inoculating leaves with the altered transcripts along with RNAs 1 and 2. Transcripts carrying internal deletions extending to within 69 residues of the 3′ poly(A) tail or to within about 300 residues of the 5′ terminus were efficiently amplified and encapsidated
in vivo. The 3′ terminal cis-essential domain can be folded into a secondary structure which is conserved among all four genomic RNAs and which probably contains the minus-strand promoter. RNA 3 transcripts with 75% of the central core of the sequence deleted or replaced by the β-glucuronidase (GUS) gene were also viable. GUS activity was detected in infected tissue in the latter case. |
| doi_str_mv | 10.1016/0042-6822(90)90403-E |
| format | Article |
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in vivo. The 3′ terminal cis-essential domain can be folded into a secondary structure which is conserved among all four genomic RNAs and which probably contains the minus-strand promoter. RNA 3 transcripts with 75% of the central core of the sequence deleted or replaced by the β-glucuronidase (GUS) gene were also viable. GUS activity was detected in infected tissue in the latter case.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(90)90403-E</identifier><identifier>PMID: 2389553</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>ARN ; Base Sequence ; Biological and medical sciences ; CHENOPODIUM QUINOA ; Chromosome Deletion ; DNA Replication ; FEUILLE ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Genetics ; Glucuronidase - biosynthesis ; Glucuronidase - genetics ; HOJAS ; LEAVES ; Microbiology ; MOLECULAR SEQUENCE DATA ; Nucleic Acid Conformation ; NUCLEOTIDE ; Nucleotide Mapping ; NUCLEOTIDES ; NUCLEOTIDOS ; PLANT VIRUSES ; Plant Viruses - genetics ; RNA ; RNA AMPLIFICATION ; RNA, Viral - biosynthesis ; Sequence Homology, Nucleic Acid ; Transcription, Genetic ; Virology ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX ; Virus Replication</subject><ispartof>Virology (New York, N.Y.), 1990-09, Vol.178 (1), p.273-280</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-e4d43561498cba55412f8910bc671b159ecdd93fc55741523cd1ee5d19e8196d3</citedby><cites>FETCH-LOGICAL-c437t-e4d43561498cba55412f8910bc671b159ecdd93fc55741523cd1ee5d19e8196d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(90)90403-E$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19526887$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2389553$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jupin, I.</creatorcontrib><creatorcontrib>Richards, K.</creatorcontrib><creatorcontrib>Jonard, G.</creatorcontrib><creatorcontrib>Guilley, H.</creatorcontrib><creatorcontrib>Pleij, C.W.A.</creatorcontrib><title>Mapping sequences required for productive replication of beet necrotic yellow vein virus RNA 3</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Of the four genome components of beet necrotic yellow vein virus only RNAs 1 and 2 are essential for viral replication in leaves. We have mapped cis-regulatory elements on RNA 3 by introducing deletions into expressible cDNA clones and inoculating leaves with the altered transcripts along with RNAs 1 and 2. Transcripts carrying internal deletions extending to within 69 residues of the 3′ poly(A) tail or to within about 300 residues of the 5′ terminus were efficiently amplified and encapsidated
in vivo. The 3′ terminal cis-essential domain can be folded into a secondary structure which is conserved among all four genomic RNAs and which probably contains the minus-strand promoter. RNA 3 transcripts with 75% of the central core of the sequence deleted or replaced by the β-glucuronidase (GUS) gene were also viable. GUS activity was detected in infected tissue in the latter case.</description><subject>ARN</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CHENOPODIUM QUINOA</subject><subject>Chromosome Deletion</subject><subject>DNA Replication</subject><subject>FEUILLE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Genetics</subject><subject>Glucuronidase - biosynthesis</subject><subject>Glucuronidase - genetics</subject><subject>HOJAS</subject><subject>LEAVES</subject><subject>Microbiology</subject><subject>MOLECULAR SEQUENCE DATA</subject><subject>Nucleic Acid Conformation</subject><subject>NUCLEOTIDE</subject><subject>Nucleotide Mapping</subject><subject>NUCLEOTIDES</subject><subject>NUCLEOTIDOS</subject><subject>PLANT VIRUSES</subject><subject>Plant Viruses - genetics</subject><subject>RNA</subject><subject>RNA AMPLIFICATION</subject><subject>RNA, Viral - biosynthesis</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Transcription, Genetic</subject><subject>Virology</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><subject>Virus Replication</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKkvhDyCQfCmCQ8Djr8QXpKraAlILEtArlmNPKqNsEuxkq_77etlVudGTP95nXs28Q8grYO-Bgf7AmOSVbjh_a9g7wyQT1foRWQEzumJCwmOyukeekmc5_2blXdfsiBxx0RilxIr8unTTFIdrmvHPgoPHTFO5xYSBdmOiUxrD4ue4xfI_9dG7OY4DHTvaIs50QJ_GOXp6i30_3tAtxoFuY1oy_f71lIrn5Enn-owvDucxuTpf_zz7XF18-_Tl7PSi8lLUc4UySKE0SNP41iklgXeNAdZ6XUMLyqAPwYjOK1VLUFz4AIgqgMEGjA7imLzZ-5Z-yxx5tpuYfenJDTgu2dbGqJpr9iAIGngtuS6g3INlwJwTdnZKcePSrQVmd_nbXbh2F641zP7N365L2euD_9JuMNwXHQIv-slBd9m7vktu8DH_8zaK66apC_dyz3VutO46FebqhwHgUkARP-5FLJFuIyabfdwtL5S9-dmGMf6_yzvbKqkk</recordid><startdate>19900901</startdate><enddate>19900901</enddate><creator>Jupin, I.</creator><creator>Richards, K.</creator><creator>Jonard, G.</creator><creator>Guilley, H.</creator><creator>Pleij, C.W.A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900901</creationdate><title>Mapping sequences required for productive replication of beet necrotic yellow vein virus RNA 3</title><author>Jupin, I. ; Richards, K. ; Jonard, G. ; Guilley, H. ; Pleij, C.W.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-e4d43561498cba55412f8910bc671b159ecdd93fc55741523cd1ee5d19e8196d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>ARN</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CHENOPODIUM QUINOA</topic><topic>Chromosome Deletion</topic><topic>DNA Replication</topic><topic>FEUILLE</topic><topic>Fundamental and applied biological sciences. 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We have mapped cis-regulatory elements on RNA 3 by introducing deletions into expressible cDNA clones and inoculating leaves with the altered transcripts along with RNAs 1 and 2. Transcripts carrying internal deletions extending to within 69 residues of the 3′ poly(A) tail or to within about 300 residues of the 5′ terminus were efficiently amplified and encapsidated
in vivo. The 3′ terminal cis-essential domain can be folded into a secondary structure which is conserved among all four genomic RNAs and which probably contains the minus-strand promoter. RNA 3 transcripts with 75% of the central core of the sequence deleted or replaced by the β-glucuronidase (GUS) gene were also viable. GUS activity was detected in infected tissue in the latter case.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2389553</pmid><doi>10.1016/0042-6822(90)90403-E</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | ARN Base Sequence Biological and medical sciences CHENOPODIUM QUINOA Chromosome Deletion DNA Replication FEUILLE Fundamental and applied biological sciences. Psychology Genes, Viral Genetics Glucuronidase - biosynthesis Glucuronidase - genetics HOJAS LEAVES Microbiology MOLECULAR SEQUENCE DATA Nucleic Acid Conformation NUCLEOTIDE Nucleotide Mapping NUCLEOTIDES NUCLEOTIDOS PLANT VIRUSES Plant Viruses - genetics RNA RNA AMPLIFICATION RNA, Viral - biosynthesis Sequence Homology, Nucleic Acid Transcription, Genetic Virology VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX Virus Replication |
| title | Mapping sequences required for productive replication of beet necrotic yellow vein virus RNA 3 |
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