Genome and antigenic analysis of influenza A (H3N2) viruses isolated from an epidemic in a closed community of Carmelite nuns
Eighteen influenza A (H3N2) viruses were isolated during a single outbreak in a closed community of Carmelite nuns. Serological analysis of the virus haemagglutinin (HAs), using a panel of monoclonal antibodies, demonstrated antigenic microheterogeneity. In contrast, no significant biochemical diffe...
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| Veröffentlicht in: | Journal of medical virology 1990-06, Vol.31 (2), p.112-119 |
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| container_title | Journal of medical virology |
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| creator | Donatelli, I. Takhonova, A. M. Klimov, A. I. Ghendon, Y. Z. Oxford, J. S. |
| description | Eighteen influenza A (H3N2) viruses were isolated during a single outbreak in a closed community of Carmelite nuns. Serological analysis of the virus haemagglutinin (HAs), using a panel of monoclonal antibodies, demonstrated antigenic microheterogeneity. In contrast, no significant biochemical differences were detected in viral genes by RNA:RNA hybridisation or in structural or nonstructural polypeptides analysed by high‐resolution polyacrylamide gel electrophoresis (PAGE) or by limited proteolysis followed by PAGE. Influenza A (H3N2) viruses isolated in the vicinity of the convent were distinguishable from each other and from the epidemic viruses isolated in the convent both antigenically and biochemically. |
| doi_str_mv | 10.1002/jmv.1890310208 |
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Influenza A (H3N2) viruses isolated in the vicinity of the convent were distinguishable from each other and from the epidemic viruses isolated in the convent both antigenically and biochemically.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.1890310208</identifier><identifier>PMID: 2388044</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Antigenic Variation ; Antigens, Viral - genetics ; Biological and medical sciences ; Disease Outbreaks ; Epidemiology ; Female ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; genetic variation ; Hemagglutinin Glycoproteins, Influenza Virus ; Hemagglutinins, Viral - analysis ; Hemagglutinins, Viral - genetics ; Humans ; influenza ; Influenza A virus - classification ; Influenza A virus - genetics ; Influenza A virus - immunology ; Influenza A virus - isolation & purification ; Influenza A Virus, H3N2 Subtype ; Influenza, Human - epidemiology ; Influenza, Human - microbiology ; Microbiology ; molecular epidemiology ; RNA, Viral - analysis ; Rome - epidemiology ; Social Isolation ; Space life sciences ; Viral Structural Proteins - genetics ; Virology</subject><ispartof>Journal of medical virology, 1990-06, Vol.31 (2), p.112-119</ispartof><rights>Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4398-a2c3a46eba81e78f28d8af73f8ace9cedff5e3ca68524739400eabe48aa56a5a3</citedby><cites>FETCH-LOGICAL-c4398-a2c3a46eba81e78f28d8af73f8ace9cedff5e3ca68524739400eabe48aa56a5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.1890310208$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.1890310208$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19564674$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2388044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Donatelli, I.</creatorcontrib><creatorcontrib>Takhonova, A. M.</creatorcontrib><creatorcontrib>Klimov, A. I.</creatorcontrib><creatorcontrib>Ghendon, Y. Z.</creatorcontrib><creatorcontrib>Oxford, J. S.</creatorcontrib><title>Genome and antigenic analysis of influenza A (H3N2) viruses isolated from an epidemic in a closed community of Carmelite nuns</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Eighteen influenza A (H3N2) viruses were isolated during a single outbreak in a closed community of Carmelite nuns. Serological analysis of the virus haemagglutinin (HAs), using a panel of monoclonal antibodies, demonstrated antigenic microheterogeneity. In contrast, no significant biochemical differences were detected in viral genes by RNA:RNA hybridisation or in structural or nonstructural polypeptides analysed by high‐resolution polyacrylamide gel electrophoresis (PAGE) or by limited proteolysis followed by PAGE. Influenza A (H3N2) viruses isolated in the vicinity of the convent were distinguishable from each other and from the epidemic viruses isolated in the convent both antigenically and biochemically.</description><subject>Adult</subject><subject>Antigenic Variation</subject><subject>Antigens, Viral - genetics</subject><subject>Biological and medical sciences</subject><subject>Disease Outbreaks</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>genetic variation</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus</subject><subject>Hemagglutinins, Viral - analysis</subject><subject>Hemagglutinins, Viral - genetics</subject><subject>Humans</subject><subject>influenza</subject><subject>Influenza A virus - classification</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - immunology</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza A Virus, H3N2 Subtype</subject><subject>Influenza, Human - epidemiology</subject><subject>Influenza, Human - microbiology</subject><subject>Microbiology</subject><subject>molecular epidemiology</subject><subject>RNA, Viral - analysis</subject><subject>Rome - epidemiology</subject><subject>Social Isolation</subject><subject>Space life sciences</subject><subject>Viral Structural Proteins - genetics</subject><subject>Virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKtvClRuSL6ByyGLHjmMfywq2oFIQWujRmnXGyCVxljgpLBL_Ha921YpTDyOP9L73xtIj5Blnc85Y-fq6u5lzbZjgrGT6AZlxZlRhWM0fkhnjUhVK8eoxOU7pmjGmTVkekaNSaM2knJG_S4x9hxRik2cM3zEGlzdotykk2nsaom8njH-AntHTc3FZvqI3YZgSJhpS38KIDfVD32UTxU1osMsBIVKgru1TFl3fdVMM43aXtoChwzaMSOMU0xPyyEOb8OnhPSFf371dLc6Li0_L94uzi8JJYXQBpRMgFa5Bc6y1L3WjwdfCa3BoHDbeVygcKF2VshZGMoawRqkBKgUViBPycp-7GfqfE6bRdiE5bFuI2E_J1sZUsmTiXpBX-YYyPIPzPeiGPqUBvd0MoYNhazmzu2JsLsbeFZMNzw_J07rD5hY_NJH1FwcdkoPWDxBdSHepplJS1TvO7LlfocXtPVfth4_f_vtDsfeGNOLvWy8MP6yqRV3Zq8ulvfryeSXfrJZ2If4B2dq3Ww</recordid><startdate>199006</startdate><enddate>199006</enddate><creator>Donatelli, I.</creator><creator>Takhonova, A. M.</creator><creator>Klimov, A. I.</creator><creator>Ghendon, Y. Z.</creator><creator>Oxford, J. S.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199006</creationdate><title>Genome and antigenic analysis of influenza A (H3N2) viruses isolated from an epidemic in a closed community of Carmelite nuns</title><author>Donatelli, I. ; Takhonova, A. M. ; Klimov, A. I. ; Ghendon, Y. Z. ; Oxford, J. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4398-a2c3a46eba81e78f28d8af73f8ace9cedff5e3ca68524739400eabe48aa56a5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adult</topic><topic>Antigenic Variation</topic><topic>Antigens, Viral - genetics</topic><topic>Biological and medical sciences</topic><topic>Disease Outbreaks</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>genetic variation</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus</topic><topic>Hemagglutinins, Viral - analysis</topic><topic>Hemagglutinins, Viral - genetics</topic><topic>Humans</topic><topic>influenza</topic><topic>Influenza A virus - classification</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - immunology</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza A Virus, H3N2 Subtype</topic><topic>Influenza, Human - epidemiology</topic><topic>Influenza, Human - microbiology</topic><topic>Microbiology</topic><topic>molecular epidemiology</topic><topic>RNA, Viral - analysis</topic><topic>Rome - epidemiology</topic><topic>Social Isolation</topic><topic>Space life sciences</topic><topic>Viral Structural Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Donatelli, I.</creatorcontrib><creatorcontrib>Takhonova, A. 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S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome and antigenic analysis of influenza A (H3N2) viruses isolated from an epidemic in a closed community of Carmelite nuns</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>1990-06</date><risdate>1990</risdate><volume>31</volume><issue>2</issue><spage>112</spage><epage>119</epage><pages>112-119</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Eighteen influenza A (H3N2) viruses were isolated during a single outbreak in a closed community of Carmelite nuns. Serological analysis of the virus haemagglutinin (HAs), using a panel of monoclonal antibodies, demonstrated antigenic microheterogeneity. In contrast, no significant biochemical differences were detected in viral genes by RNA:RNA hybridisation or in structural or nonstructural polypeptides analysed by high‐resolution polyacrylamide gel electrophoresis (PAGE) or by limited proteolysis followed by PAGE. Influenza A (H3N2) viruses isolated in the vicinity of the convent were distinguishable from each other and from the epidemic viruses isolated in the convent both antigenically and biochemically.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2388044</pmid><doi>10.1002/jmv.1890310208</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Antigenic Variation Antigens, Viral - genetics Biological and medical sciences Disease Outbreaks Epidemiology Female Fundamental and applied biological sciences. Psychology Genes, Viral genetic variation Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral - analysis Hemagglutinins, Viral - genetics Humans influenza Influenza A virus - classification Influenza A virus - genetics Influenza A virus - immunology Influenza A virus - isolation & purification Influenza A Virus, H3N2 Subtype Influenza, Human - epidemiology Influenza, Human - microbiology Microbiology molecular epidemiology RNA, Viral - analysis Rome - epidemiology Social Isolation Space life sciences Viral Structural Proteins - genetics Virology |
| title | Genome and antigenic analysis of influenza A (H3N2) viruses isolated from an epidemic in a closed community of Carmelite nuns |
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