Diversity of single potassium channels in isolated snail neurons
COMPARISON of K+ channels in mollusk and mammalian neurons has been made to elucidate their fundamental properties. Using patch clamp cell-attached configuration, K channels in isolated snail neurons were separated into three subtypeswith big (BKC), medium (MKC) and small (SKC) unitary conductances....
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| Veröffentlicht in: | Neuroreport 1998-05, Vol.9 (7), p.1413-1417 |
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| creator | Sotkis, A V Kostyuk, P G Lukyanetz, E A |
| description | COMPARISON of K+ channels in mollusk and mammalian neurons has been made to elucidate their fundamental properties. Using patch clamp cell-attached configuration, K channels in isolated snail neurons were separated into three subtypeswith big (BKC), medium (MKC) and small (SKC) unitary conductances. BKC and MKC were activated at −30 mV and SKC at more negative potentials. BKC and MKC proved sensitive to TEA, whereas SKC were sensitive to 4-AP. Cd in the pipet decreased unitary conductance of BKC by 55% and of MKC by about 31%. Bath application of 5-HT selectively suppressed MKC. It is suggested that BKC can be referred to large conductance Ca-dependent K currents (KCa), MKC to intermediate conductance KCa and SKC channels comply with the characteristics of A current of mammals. These data show that KCa and A currents may be the most general types of currents generated by K channels. |
| doi_str_mv | 10.1097/00001756-199805110-00030 |
| format | Article |
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Using patch clamp cell-attached configuration, K channels in isolated snail neurons were separated into three subtypeswith big (BKC), medium (MKC) and small (SKC) unitary conductances. BKC and MKC were activated at −30 mV and SKC at more negative potentials. BKC and MKC proved sensitive to TEA, whereas SKC were sensitive to 4-AP. Cd in the pipet decreased unitary conductance of BKC by 55% and of MKC by about 31%. Bath application of 5-HT selectively suppressed MKC. It is suggested that BKC can be referred to large conductance Ca-dependent K currents (KCa), MKC to intermediate conductance KCa and SKC channels comply with the characteristics of A current of mammals. 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Using patch clamp cell-attached configuration, K channels in isolated snail neurons were separated into three subtypeswith big (BKC), medium (MKC) and small (SKC) unitary conductances. BKC and MKC were activated at −30 mV and SKC at more negative potentials. BKC and MKC proved sensitive to TEA, whereas SKC were sensitive to 4-AP. Cd in the pipet decreased unitary conductance of BKC by 55% and of MKC by about 31%. Bath application of 5-HT selectively suppressed MKC. It is suggested that BKC can be referred to large conductance Ca-dependent K currents (KCa), MKC to intermediate conductance KCa and SKC channels comply with the characteristics of A current of mammals. These data show that KCa and A currents may be the most general types of currents generated by K channels.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>Brackish</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastropoda</subject><subject>Helix (Snails)</subject><subject>In Vitro Techniques</subject><subject>Invertebrates</subject><subject>Mammals</subject><subject>Marine</subject><subject>Membrane Potentials - drug effects</subject><subject>Mollusca</subject><subject>Neurons - physiology</subject><subject>Patch-Clamp Techniques</subject><subject>Physiology. 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Development</topic><topic>Potassium Channels - classification</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>Serotonin - pharmacology</topic><topic>Tetraethylammonium - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sotkis, A V</creatorcontrib><creatorcontrib>Kostyuk, P G</creatorcontrib><creatorcontrib>Lukyanetz, E A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sotkis, A V</au><au>Kostyuk, P G</au><au>Lukyanetz, E A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity of single potassium channels in isolated snail neurons</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>1998-05-11</date><risdate>1998</risdate><volume>9</volume><issue>7</issue><spage>1413</spage><epage>1417</epage><pages>1413-1417</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>COMPARISON of K+ channels in mollusk and mammalian neurons has been made to elucidate their fundamental properties. 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| subjects | Animals Biochemistry. Physiology. Immunology Biological and medical sciences Brackish Freshwater Fundamental and applied biological sciences. Psychology Gastropoda Helix (Snails) In Vitro Techniques Invertebrates Mammals Marine Membrane Potentials - drug effects Mollusca Neurons - physiology Patch-Clamp Techniques Physiology. Development Potassium Channels - classification Potassium Channels - drug effects Potassium Channels - physiology Serotonin - pharmacology Tetraethylammonium - pharmacology |
| title | Diversity of single potassium channels in isolated snail neurons |
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