DNA sequences from Saccharomycopsis fibuligera capable of autonomous replication in Saccharomyces cerevisiae
A Sau 3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast‐integrating plasmid YEp24ΔEcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from...
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| Veröffentlicht in: | Journal of basic microbiology 1990, Vol.30 (4), p.301-304 |
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| container_title | Journal of basic microbiology |
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| creator | Jursky, F Hostinova, E |
| description | A Sau 3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast‐integrating plasmid YEp24ΔEcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saccharomycopsis fibuligera with ARS activity in Saccharomyces cerevisiae. |
| doi_str_mv | 10.1002/jobm.3620300419 |
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From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saccharomycopsis fibuligera with ARS activity in Saccharomyces cerevisiae.</description><identifier>ISSN: 0233-111X</identifier><identifier>EISSN: 1521-4028</identifier><identifier>DOI: 10.1002/jobm.3620300419</identifier><identifier>PMID: 2200870</identifier><identifier>CODEN: JBMIEQ</identifier><language>eng</language><publisher>Berlin: Wiley-VCH</publisher><subject>Base Sequence ; Biological and medical sciences ; cloning ; DNA ; DNA Replication ; DNA, Fungal - analysis ; Fundamental and applied biological sciences. 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Basic Microbiol</addtitle><description>A Sau 3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast‐integrating plasmid YEp24ΔEcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saccharomycopsis fibuligera with ARS activity in Saccharomyces cerevisiae.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cloning</subject><subject>DNA</subject><subject>DNA Replication</subject><subject>DNA, Fungal - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>nucleotide sequences</subject><subject>plasmids</subject><subject>Replication</subject><subject>Saccharomyces</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomycetales - genetics</subject><subject>Saccharomycopsis - genetics</subject><subject>Saccharomycopsis fibuligera</subject><subject>transformation</subject><subject>Transformation, Genetic</subject><issn>0233-111X</issn><issn>1521-4028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKkvhzAmRC9zSju11HItTKdAFlvZQCoiLNfY6xW0SB3sD7L_Hq6xaOFWyZMvzzZs3M4Q8pXBAAdjhVTDdAa8YcIA5VffIjApGyzmw-j6ZAeO8pJR-e0gepXQFAEoxtUf2GAOoJcxI--b0qEju5-h661LRxNAV52jtD8yvjQ1D8vnXm7H1ly5iYXFA07oiNAWO69CHLoypiG5ovcW1D33h-38FsqZ10f3yyaN7TB402Cb3ZHfvk4t3bz8fL8rl2cn746NlaeeMqrICwXhuxtRYr4zkCkVdWaEYq6miYgUolG0spbASleSVqZgFLg03dgWWG75PXk66Qwy5s7TWnU_WtS32LtvVUqltIXUnSEXNpJBVBg8n0MaQUnSNHqLvMG40Bb1dhN4uQt8uImc820mPpnOrG343-Rx_sYtjstg2EXvr062s4lApzjL3auJ--9Zt7iqrP5y9_vSfi3LK9mnt_txkY7zWeXJS6K-nJ5rS5cfviy8LPc_884lvMGi8jNnRxTkDyoHJfGTN_wJesrtN</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Jursky, F</creator><creator>Hostinova, E</creator><general>Wiley-VCH</general><general>Wiley‐VCH</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>DNA sequences from Saccharomycopsis fibuligera capable of autonomous replication in Saccharomyces cerevisiae</title><author>Jursky, F ; Hostinova, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4219-60523419b8a8db739a586c592281915d0a59cfc110d56736b62c037b3bcd0c3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cloning</topic><topic>DNA</topic><topic>DNA Replication</topic><topic>DNA, Fungal - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>nucleotide sequences</topic><topic>plasmids</topic><topic>Replication</topic><topic>Saccharomyces</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomycetales - genetics</topic><topic>Saccharomycopsis - genetics</topic><topic>Saccharomycopsis fibuligera</topic><topic>transformation</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jursky, F</creatorcontrib><creatorcontrib>Hostinova, E</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of basic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jursky, F</au><au>Hostinova, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA sequences from Saccharomycopsis fibuligera capable of autonomous replication in Saccharomyces cerevisiae</atitle><jtitle>Journal of basic microbiology</jtitle><addtitle>J. Basic Microbiol</addtitle><date>1990</date><risdate>1990</risdate><volume>30</volume><issue>4</issue><spage>301</spage><epage>304</epage><pages>301-304</pages><issn>0233-111X</issn><eissn>1521-4028</eissn><coden>JBMIEQ</coden><abstract>A Sau 3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast‐integrating plasmid YEp24ΔEcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saccharomycopsis fibuligera with ARS activity in Saccharomyces cerevisiae.</abstract><cop>Berlin</cop><pub>Wiley-VCH</pub><pmid>2200870</pmid><doi>10.1002/jobm.3620300419</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of basic microbiology, 1990, Vol.30 (4), p.301-304 |
| issn | 0233-111X 1521-4028 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Base Sequence Biological and medical sciences cloning DNA DNA Replication DNA, Fungal - analysis Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics nucleotide sequences plasmids Replication Saccharomyces Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomycetales - genetics Saccharomycopsis - genetics Saccharomycopsis fibuligera transformation Transformation, Genetic |
| title | DNA sequences from Saccharomycopsis fibuligera capable of autonomous replication in Saccharomyces cerevisiae |
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