$A fractionated reticulocyte lysate retains high efficiency for protein synthesis$

A fractionated reticulocyte lysate retains high efficiency for protein synthesis

The rabbit reticulocyte lysate is a highly efficient system for protein synthesis in vitro, and therefore has been used frequently in studies of translational control. However, when the lysate is fractionated into ribosomes and soluble proteins by centrifugation, there is a severe loss of activity,...

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Veröffentlicht in:	Biochimie 1990-04, Vol.72 (4), p.259-264
Hauptverfasser:	Morley, S.J., Hershey, J.W.B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Centrifugation Electrophoresis, Polyacrylamide Gel Eukaryotic Initiation Factor-4F Eukaryotic Initiation Factors Fundamental and applied biological sciences. Psychology Gene Expression Regulation in vitro protein synthesis In Vitro Techniques Methionine - metabolism Molecular and cellular biology Molecular genetics Peptide Initiation Factors - genetics Peptide Initiation Factors - metabolism Protein Biosynthesis rabbit reticulocyte lysate Rabbits Reticulocytes - metabolism Ribosomes - metabolism Translation. Translation factors. Protein processing translational control studies
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