Volume-Referent Bone Turnover Estimated From the Interlabel Area Fraction After Sequential Labeling
In the compact bony otic capsule remodeling is low, and bone remodeling units are distributed centrifugally in relation to inner ear tissues and spaces. Fluorochrome-labeled bone remodeling units are scarce, abortive, and tortuous with no uniform direction of movement. This study presents a method f...
Gespeichert in:
| Veröffentlicht in: | Bone (New York, N.Y.) N.Y.), 1998-06, Vol.22 (6), p.677-682 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 682 |
|---|---|
| container_issue | 6 |
| container_start_page | 677 |
| container_title | Bone (New York, N.Y.) |
| container_volume | 22 |
| creator | Frisch, T Sørensen, M.S Overgaard, S Lind, M Bretlau, P |
| description | In the compact bony otic capsule remodeling is low, and bone remodeling units are distributed centrifugally in relation to inner ear tissues and spaces. Fluorochrome-labeled bone remodeling units are scarce, abortive, and tortuous with no uniform direction of movement. This study presents a method for the estimation of volume-referent bone turnover based on measurements of the fractional area between labels after sequential labeling with osteofluorochromes. The applicability of this method is tested against a classical quantification method in undecalcified cortical specimens from the canine humerus, where both methods can be used. The estimate of bone turnover derived from the new sequential labeling in eight dogs is 7.4% (SEM 2.1%) per year and the classic estimate derived from calculations of the formative osteonal area and the formative period yields 6.9% (SEM 2.1%) per year. Agreement is sufficient to justify future measurements of absolute bone turnover in sequentially labeled perilabyrinthine bone. |
| doi_str_mv | 10.1016/S8756-3282(98)00050-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79940025</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S8756328298000507</els_id><sourcerecordid>79940025</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-e7a149a371c8aeb9ca544c678dddecae1ff816e67a77b327653c1100f968e3e53</originalsourceid><addsrcrecordid>eNqFkEtP3DAURq2KapjS_oSRvKgQXaTYceLHCg1oaJFGqgS0W8vj3BRXiQ22g8S_x_PQbLu6i-_c10FoQcl3Sii_fJCi5RWrZX2h5DdCSEsq8QHNqRSsqgVnJ2h-RE7Rp5T-FYgpQWdopnjNGyLnyP4JwzRCdQ89RPAZXwcP-HGKPrxCxKuU3WgydPg2hhHnJ8B3PkMczAYGvIxgSmBsdsHjZV8C_AAvU5njzIDXW8j5v5_Rx94MCb4c6hn6fbt6vPlZrX_9uLtZrivbNCxXIAxtlGGCWmlgo6xpm8ZyIbuuA2uA9r2kHLgwQmxY-bBlllJCesUlMGjZGTrfz32OoRyRsh5dsjAMxkOYkhZKNYTUW7DdgzaGlCL0-jmWN-ObpkRv5eqdXL01p5XUO7lalL7FYcG0GaE7dh1slvzrITfJmqGPxluXjlhdt0LSpmBXewyKjFcHUSfrwFvoXASbdRfcfw55B8Gtlrg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79940025</pqid></control><display><type>article</type><title>Volume-Referent Bone Turnover Estimated From the Interlabel Area Fraction After Sequential Labeling</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Frisch, T ; Sørensen, M.S ; Overgaard, S ; Lind, M ; Bretlau, P</creator><creatorcontrib>Frisch, T ; Sørensen, M.S ; Overgaard, S ; Lind, M ; Bretlau, P</creatorcontrib><description>In the compact bony otic capsule remodeling is low, and bone remodeling units are distributed centrifugally in relation to inner ear tissues and spaces. Fluorochrome-labeled bone remodeling units are scarce, abortive, and tortuous with no uniform direction of movement. This study presents a method for the estimation of volume-referent bone turnover based on measurements of the fractional area between labels after sequential labeling with osteofluorochromes. The applicability of this method is tested against a classical quantification method in undecalcified cortical specimens from the canine humerus, where both methods can be used. The estimate of bone turnover derived from the new sequential labeling in eight dogs is 7.4% (SEM 2.1%) per year and the classic estimate derived from calculations of the formative osteonal area and the formative period yields 6.9% (SEM 2.1%) per year. Agreement is sufficient to justify future measurements of absolute bone turnover in sequentially labeled perilabyrinthine bone.</description><identifier>ISSN: 8756-3282</identifier><identifier>EISSN: 1873-2763</identifier><identifier>DOI: 10.1016/S8756-3282(98)00050-7</identifier><identifier>PMID: 9626408</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Bone Density - physiology ; Bone remodeling ; Bone Remodeling - physiology ; Bone Resorption - physiopathology ; Bone turnover ; Calcein ; Cortical bone ; Dogs ; Fluoresceins ; Fluorescent Dyes ; Forelimb ; Fundamental and applied biological sciences. Psychology ; Histomorphometry ; Humerus - anatomy & histology ; Male ; Microscopy, Fluorescence - methods ; Models, Biological ; Skeleton and joints ; Space life sciences ; Tetracycline ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Bone (New York, N.Y.), 1998-06, Vol.22 (6), p.677-682</ispartof><rights>1998 Elsevier Science Inc.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-e7a149a371c8aeb9ca544c678dddecae1ff816e67a77b327653c1100f968e3e53</citedby><cites>FETCH-LOGICAL-c443t-e7a149a371c8aeb9ca544c678dddecae1ff816e67a77b327653c1100f968e3e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S8756-3282(98)00050-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2257814$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9626408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frisch, T</creatorcontrib><creatorcontrib>Sørensen, M.S</creatorcontrib><creatorcontrib>Overgaard, S</creatorcontrib><creatorcontrib>Lind, M</creatorcontrib><creatorcontrib>Bretlau, P</creatorcontrib><title>Volume-Referent Bone Turnover Estimated From the Interlabel Area Fraction After Sequential Labeling</title><title>Bone (New York, N.Y.)</title><addtitle>Bone</addtitle><description>In the compact bony otic capsule remodeling is low, and bone remodeling units are distributed centrifugally in relation to inner ear tissues and spaces. Fluorochrome-labeled bone remodeling units are scarce, abortive, and tortuous with no uniform direction of movement. This study presents a method for the estimation of volume-referent bone turnover based on measurements of the fractional area between labels after sequential labeling with osteofluorochromes. The applicability of this method is tested against a classical quantification method in undecalcified cortical specimens from the canine humerus, where both methods can be used. The estimate of bone turnover derived from the new sequential labeling in eight dogs is 7.4% (SEM 2.1%) per year and the classic estimate derived from calculations of the formative osteonal area and the formative period yields 6.9% (SEM 2.1%) per year. Agreement is sufficient to justify future measurements of absolute bone turnover in sequentially labeled perilabyrinthine bone.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Density - physiology</subject><subject>Bone remodeling</subject><subject>Bone Remodeling - physiology</subject><subject>Bone Resorption - physiopathology</subject><subject>Bone turnover</subject><subject>Calcein</subject><subject>Cortical bone</subject><subject>Dogs</subject><subject>Fluoresceins</subject><subject>Fluorescent Dyes</subject><subject>Forelimb</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histomorphometry</subject><subject>Humerus - anatomy & histology</subject><subject>Male</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Models, Biological</subject><subject>Skeleton and joints</subject><subject>Space life sciences</subject><subject>Tetracycline</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>8756-3282</issn><issn>1873-2763</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtP3DAURq2KapjS_oSRvKgQXaTYceLHCg1oaJFGqgS0W8vj3BRXiQ22g8S_x_PQbLu6i-_c10FoQcl3Sii_fJCi5RWrZX2h5DdCSEsq8QHNqRSsqgVnJ2h-RE7Rp5T-FYgpQWdopnjNGyLnyP4JwzRCdQ89RPAZXwcP-HGKPrxCxKuU3WgydPg2hhHnJ8B3PkMczAYGvIxgSmBsdsHjZV8C_AAvU5njzIDXW8j5v5_Rx94MCb4c6hn6fbt6vPlZrX_9uLtZrivbNCxXIAxtlGGCWmlgo6xpm8ZyIbuuA2uA9r2kHLgwQmxY-bBlllJCesUlMGjZGTrfz32OoRyRsh5dsjAMxkOYkhZKNYTUW7DdgzaGlCL0-jmWN-ObpkRv5eqdXL01p5XUO7lalL7FYcG0GaE7dh1slvzrITfJmqGPxluXjlhdt0LSpmBXewyKjFcHUSfrwFvoXASbdRfcfw55B8Gtlrg</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Frisch, T</creator><creator>Sørensen, M.S</creator><creator>Overgaard, S</creator><creator>Lind, M</creator><creator>Bretlau, P</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980601</creationdate><title>Volume-Referent Bone Turnover Estimated From the Interlabel Area Fraction After Sequential Labeling</title><author>Frisch, T ; Sørensen, M.S ; Overgaard, S ; Lind, M ; Bretlau, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-e7a149a371c8aeb9ca544c678dddecae1ff816e67a77b327653c1100f968e3e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Density - physiology</topic><topic>Bone remodeling</topic><topic>Bone Remodeling - physiology</topic><topic>Bone Resorption - physiopathology</topic><topic>Bone turnover</topic><topic>Calcein</topic><topic>Cortical bone</topic><topic>Dogs</topic><topic>Fluoresceins</topic><topic>Fluorescent Dyes</topic><topic>Forelimb</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histomorphometry</topic><topic>Humerus - anatomy & histology</topic><topic>Male</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Models, Biological</topic><topic>Skeleton and joints</topic><topic>Space life sciences</topic><topic>Tetracycline</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frisch, T</creatorcontrib><creatorcontrib>Sørensen, M.S</creatorcontrib><creatorcontrib>Overgaard, S</creatorcontrib><creatorcontrib>Lind, M</creatorcontrib><creatorcontrib>Bretlau, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bone (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frisch, T</au><au>Sørensen, M.S</au><au>Overgaard, S</au><au>Lind, M</au><au>Bretlau, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Volume-Referent Bone Turnover Estimated From the Interlabel Area Fraction After Sequential Labeling</atitle><jtitle>Bone (New York, N.Y.)</jtitle><addtitle>Bone</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>22</volume><issue>6</issue><spage>677</spage><epage>682</epage><pages>677-682</pages><issn>8756-3282</issn><eissn>1873-2763</eissn><abstract>In the compact bony otic capsule remodeling is low, and bone remodeling units are distributed centrifugally in relation to inner ear tissues and spaces. Fluorochrome-labeled bone remodeling units are scarce, abortive, and tortuous with no uniform direction of movement. This study presents a method for the estimation of volume-referent bone turnover based on measurements of the fractional area between labels after sequential labeling with osteofluorochromes. The applicability of this method is tested against a classical quantification method in undecalcified cortical specimens from the canine humerus, where both methods can be used. The estimate of bone turnover derived from the new sequential labeling in eight dogs is 7.4% (SEM 2.1%) per year and the classic estimate derived from calculations of the formative osteonal area and the formative period yields 6.9% (SEM 2.1%) per year. Agreement is sufficient to justify future measurements of absolute bone turnover in sequentially labeled perilabyrinthine bone.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9626408</pmid><doi>10.1016/S8756-3282(98)00050-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 8756-3282 |
| ispartof | Bone (New York, N.Y.), 1998-06, Vol.22 (6), p.677-682 |
| issn | 8756-3282 1873-2763 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79940025 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Bone Density - physiology Bone remodeling Bone Remodeling - physiology Bone Resorption - physiopathology Bone turnover Calcein Cortical bone Dogs Fluoresceins Fluorescent Dyes Forelimb Fundamental and applied biological sciences. Psychology Histomorphometry Humerus - anatomy & histology Male Microscopy, Fluorescence - methods Models, Biological Skeleton and joints Space life sciences Tetracycline Vertebrates: osteoarticular system, musculoskeletal system |
| title | Volume-Referent Bone Turnover Estimated From the Interlabel Area Fraction After Sequential Labeling |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T12%3A40%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Volume-Referent%20Bone%20Turnover%20Estimated%20From%20the%20Interlabel%20Area%20Fraction%20After%20Sequential%20Labeling&rft.jtitle=Bone%20(New%20York,%20N.Y.)&rft.au=Frisch,%20T&rft.date=1998-06-01&rft.volume=22&rft.issue=6&rft.spage=677&rft.epage=682&rft.pages=677-682&rft.issn=8756-3282&rft.eissn=1873-2763&rft_id=info:doi/10.1016/S8756-3282(98)00050-7&rft_dat=%3Cproquest_cross%3E79940025%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79940025&rft_id=info:pmid/9626408&rft_els_id=S8756328298000507&rfr_iscdi=true |