Molecular characterization of nuclear basic protein HPI1, a putative precursor of human sperm protamines HP2 and HP3
The largest intermediate basic protein HPI1 (101 residues) from human sperm chromatin was isolated and characterized. The amino acid composition and sequence analysis of the protein and of tryptic peptides together with peptide mapping of endoproteinases Lys‐C and Glu‐C hydrolysates showed that the...
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| Veröffentlicht in: | European journal of biochemistry 1990-07, Vol.191 (2), p.449-451 |
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| container_end_page | 451 |
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| container_issue | 2 |
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| container_title | European journal of biochemistry |
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| creator | MARTINAGE, Arlette ARKHIS, Ahmed ALIMI, Elisabeth SAUTIÈRE, Pierre CHEVAILLIER, Philippe |
| description | The largest intermediate basic protein HPI1 (101 residues) from human sperm chromatin was isolated and characterized. The amino acid composition and sequence analysis of the protein and of tryptic peptides together with peptide mapping of endoproteinases Lys‐C and Glu‐C hydrolysates showed that the C‐terminal region (residues 45 – 101) of HPI1 is identical to protamine HP2. These structural data strongly suggest that protein HPI1 is a precursor of human sperm protamines HP2 and HP3 (57 and 54 residues, respectively) as well as of two other intermediate basic proteins HPS1 and HPS2 (69 and 66 residues, respectively) sequenced previously. |
| doi_str_mv | 10.1111/j.1432-1033.1990.tb19142.x |
| format | Article |
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The amino acid composition and sequence analysis of the protein and of tryptic peptides together with peptide mapping of endoproteinases Lys‐C and Glu‐C hydrolysates showed that the C‐terminal region (residues 45 – 101) of HPI1 is identical to protamine HP2. These structural data strongly suggest that protein HPI1 is a precursor of human sperm protamines HP2 and HP3 (57 and 54 residues, respectively) as well as of two other intermediate basic proteins HPS1 and HPS2 (69 and 66 residues, respectively) sequenced previously.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1990.tb19142.x</identifier><identifier>PMID: 2384091</identifier><identifier>CODEN: EJBCAI</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Holoproteins ; HPI1 protein ; Humans ; Male ; Molecular Sequence Data ; nuclear basic protein ; Nuclear proteins ; Nuclear Proteins - analysis ; Peptide Mapping ; protamines ; Protamines - isolation & purification ; Protein Precursors - isolation & purification ; Proteins ; Spermatozoa - analysis ; Trypsin</subject><ispartof>European journal of biochemistry, 1990-07, Vol.191 (2), p.449-451</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4979-50f1b671bf432fe59b5f9557432766c0ade8569c003f3fa4840322d6048902a23</citedby><cites>FETCH-LOGICAL-c4979-50f1b671bf432fe59b5f9557432766c0ade8569c003f3fa4840322d6048902a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19485700$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2384091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MARTINAGE, Arlette</creatorcontrib><creatorcontrib>ARKHIS, Ahmed</creatorcontrib><creatorcontrib>ALIMI, Elisabeth</creatorcontrib><creatorcontrib>SAUTIÈRE, Pierre</creatorcontrib><creatorcontrib>CHEVAILLIER, Philippe</creatorcontrib><title>Molecular characterization of nuclear basic protein HPI1, a putative precursor of human sperm protamines HP2 and HP3</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>The largest intermediate basic protein HPI1 (101 residues) from human sperm chromatin was isolated and characterized. The amino acid composition and sequence analysis of the protein and of tryptic peptides together with peptide mapping of endoproteinases Lys‐C and Glu‐C hydrolysates showed that the C‐terminal region (residues 45 – 101) of HPI1 is identical to protamine HP2. These structural data strongly suggest that protein HPI1 is a precursor of human sperm protamines HP2 and HP3 (57 and 54 residues, respectively) as well as of two other intermediate basic proteins HPS1 and HPS2 (69 and 66 residues, respectively) sequenced previously.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Holoproteins</subject><subject>HPI1 protein</subject><subject>Humans</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>nuclear basic protein</subject><subject>Nuclear proteins</subject><subject>Nuclear Proteins - analysis</subject><subject>Peptide Mapping</subject><subject>protamines</subject><subject>Protamines - isolation & purification</subject><subject>Protein Precursors - isolation & purification</subject><subject>Proteins</subject><subject>Spermatozoa - analysis</subject><subject>Trypsin</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkVtrFTEUhYMo9Xj0JwhB0CdnzHVmti9FS2sLFQX1OezJSWgOczkmM9r66814hvoo5mUT1lr7wkfIC85Knt-bfcmVFAVnUpYcgJVTy4ErUd4-IJt76SHZMMZVIUBXj8mTlPaMsQqq-oScCNkoBnxDpo9j5-zcYaT2BiPaycXwC6cwDnT0dJht57LWYgqWHuI4uTDQy89X_DVFepin7PzhspB7xDTGJXMz9zjQdHCx_5PAPgwu5ZCgOOxylU_JI49dcs_WuiXfLs6_nl0W158-XJ29uy6sghoKzTxvq5q3Pp_knYZWe9C6zr-6qizDnWt0BZYx6aVHlU-SQuwqphpgAoXcklfHvnmN77NLk-lDsq7rcHDjnEwNIBWI5p9GrpuG1xKy8e3RaOOYUnTeHGLoMd4ZzszCxuzNAsAsAMzCxqxszG0OP1-nzG3vdvfRFUbWX646JoudjzjYkP5OANXoOh-7JadH38_Qubv_2MBcnL__ohTI32Blqqk</recordid><startdate>19900731</startdate><enddate>19900731</enddate><creator>MARTINAGE, Arlette</creator><creator>ARKHIS, Ahmed</creator><creator>ALIMI, Elisabeth</creator><creator>SAUTIÈRE, Pierre</creator><creator>CHEVAILLIER, Philippe</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19900731</creationdate><title>Molecular characterization of nuclear basic protein HPI1, a putative precursor of human sperm protamines HP2 and HP3</title><author>MARTINAGE, Arlette ; ARKHIS, Ahmed ; ALIMI, Elisabeth ; SAUTIÈRE, Pierre ; CHEVAILLIER, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4979-50f1b671bf432fe59b5f9557432766c0ade8569c003f3fa4840322d6048902a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Holoproteins</topic><topic>HPI1 protein</topic><topic>Humans</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>nuclear basic protein</topic><topic>Nuclear proteins</topic><topic>Nuclear Proteins - analysis</topic><topic>Peptide Mapping</topic><topic>protamines</topic><topic>Protamines - isolation & purification</topic><topic>Protein Precursors - isolation & purification</topic><topic>Proteins</topic><topic>Spermatozoa - analysis</topic><topic>Trypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MARTINAGE, Arlette</creatorcontrib><creatorcontrib>ARKHIS, Ahmed</creatorcontrib><creatorcontrib>ALIMI, Elisabeth</creatorcontrib><creatorcontrib>SAUTIÈRE, Pierre</creatorcontrib><creatorcontrib>CHEVAILLIER, Philippe</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MARTINAGE, Arlette</au><au>ARKHIS, Ahmed</au><au>ALIMI, Elisabeth</au><au>SAUTIÈRE, Pierre</au><au>CHEVAILLIER, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of nuclear basic protein HPI1, a putative precursor of human sperm protamines HP2 and HP3</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1990-07-31</date><risdate>1990</risdate><volume>191</volume><issue>2</issue><spage>449</spage><epage>451</epage><pages>449-451</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><coden>EJBCAI</coden><abstract>The largest intermediate basic protein HPI1 (101 residues) from human sperm chromatin was isolated and characterized. The amino acid composition and sequence analysis of the protein and of tryptic peptides together with peptide mapping of endoproteinases Lys‐C and Glu‐C hydrolysates showed that the C‐terminal region (residues 45 – 101) of HPI1 is identical to protamine HP2. These structural data strongly suggest that protein HPI1 is a precursor of human sperm protamines HP2 and HP3 (57 and 54 residues, respectively) as well as of two other intermediate basic proteins HPS1 and HPS2 (69 and 66 residues, respectively) sequenced previously.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2384091</pmid><doi>10.1111/j.1432-1033.1990.tb19142.x</doi><tpages>3</tpages></addata></record> |
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| ispartof | European journal of biochemistry, 1990-07, Vol.191 (2), p.449-451 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Biological and medical sciences Fundamental and applied biological sciences. Psychology Holoproteins HPI1 protein Humans Male Molecular Sequence Data nuclear basic protein Nuclear proteins Nuclear Proteins - analysis Peptide Mapping protamines Protamines - isolation & purification Protein Precursors - isolation & purification Proteins Spermatozoa - analysis Trypsin |
| title | Molecular characterization of nuclear basic protein HPI1, a putative precursor of human sperm protamines HP2 and HP3 |
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