Effect on colon cancer cells of human interferon‐β gene entrapped in cationic multilamellar liposomes
When cultured cells of human colon cancer cell line SW480 were transfected with human interferon‐β (hIFN‐β) gene by means of cationic multilamellar liposomes, the endogenously produced hIFN‐β exhibited a remarkable anti‐proliferative effect on the cells, which was more effective than that of exogeno...
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| Veröffentlicht in: | Biochemistry and molecular biology international 1998-05, Vol.44 (6), p.1235-1243 |
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| container_title | Biochemistry and molecular biology international |
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| creator | Shimizu, Minoru Akiyama, Seiji Ito, Katsuki Kasai, Yasushi Takagi, Hiroshi Kito, Mariko Ohishi, Nobuko Yagi, Kunio |
| description | When cultured cells of human colon cancer cell line SW480 were transfected with human interferon‐β (hIFN‐β) gene by means of cationic multilamellar liposomes, the endogenously produced hIFN‐β exhibited a remarkable anti‐proliferative effect on the cells, which was more effective than that of exogenously added hIFN‐β. This effect lasted for several days, and was blocked completely by the addition of sufficient amounts of anti‐hIFN‐β antibody. From experiments using a transwell plate and an infusion pump, we found that endogenously produced hIFN‐β acted effectively on the cells around the transfectants and that the growth‐inhibitory effect was totally retained upon continuous dilution of the medium. These data indicate that hIFN‐β expressed endogenously by transfer of its gene acted on these cancer cells mainly in a paracrine manner. Although the transfection with hIFN‐γ gene also revealed a definite growth‐inhibitory effect on the same tumor cells, the extent was less than that of hIFN‐β gene. |
| doi_str_mv | 10.1080/15216549800202332 |
| format | Article |
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This effect lasted for several days, and was blocked completely by the addition of sufficient amounts of anti‐hIFN‐β antibody. From experiments using a transwell plate and an infusion pump, we found that endogenously produced hIFN‐β acted effectively on the cells around the transfectants and that the growth‐inhibitory effect was totally retained upon continuous dilution of the medium. These data indicate that hIFN‐β expressed endogenously by transfer of its gene acted on these cancer cells mainly in a paracrine manner. Although the transfection with hIFN‐γ gene also revealed a definite growth‐inhibitory effect on the same tumor cells, the extent was less than that of hIFN‐β gene.</description><identifier>ISSN: 1521-6543</identifier><identifier>ISSN: 1039-9712</identifier><identifier>EISSN: 1521-6551</identifier><identifier>DOI: 10.1080/15216549800202332</identifier><identifier>PMID: 9623779</identifier><language>eng</language><publisher>UK: Informa Healthcare</publisher><subject>Antibodies - pharmacology ; cationic multilamellar liposomes ; Cell Division - physiology ; colon cancer cells ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - secretion ; Colonic Neoplasms - therapy ; DNA, Complementary - administration & dosage ; DNA, Complementary - genetics ; Genetic Therapy - methods ; Humans ; IFN‐β cDNA ; Interferon-beta - biosynthesis ; Interferon-beta - genetics ; Interferon-beta - secretion ; Interferon-gamma - genetics ; Liposomes ; Transfection ; Tumor Cells, Cultured</subject><ispartof>Biochemistry and molecular biology international, 1998-05, Vol.44 (6), p.1235-1243</ispartof><rights>Copyright © 1998 International Union of Biochemistry and Molecular Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1080%2F15216549800202332$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1080%2F15216549800202332$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9623779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimizu, Minoru</creatorcontrib><creatorcontrib>Akiyama, Seiji</creatorcontrib><creatorcontrib>Ito, Katsuki</creatorcontrib><creatorcontrib>Kasai, Yasushi</creatorcontrib><creatorcontrib>Takagi, Hiroshi</creatorcontrib><creatorcontrib>Kito, Mariko</creatorcontrib><creatorcontrib>Ohishi, Nobuko</creatorcontrib><creatorcontrib>Yagi, Kunio</creatorcontrib><title>Effect on colon cancer cells of human interferon‐β gene entrapped in cationic multilamellar liposomes</title><title>Biochemistry and molecular biology international</title><addtitle>Biochem Mol Biol Int</addtitle><description>When cultured cells of human colon cancer cell line SW480 were transfected with human interferon‐β (hIFN‐β) gene by means of cationic multilamellar liposomes, the endogenously produced hIFN‐β exhibited a remarkable anti‐proliferative effect on the cells, which was more effective than that of exogenously added hIFN‐β. This effect lasted for several days, and was blocked completely by the addition of sufficient amounts of anti‐hIFN‐β antibody. From experiments using a transwell plate and an infusion pump, we found that endogenously produced hIFN‐β acted effectively on the cells around the transfectants and that the growth‐inhibitory effect was totally retained upon continuous dilution of the medium. These data indicate that hIFN‐β expressed endogenously by transfer of its gene acted on these cancer cells mainly in a paracrine manner. Although the transfection with hIFN‐γ gene also revealed a definite growth‐inhibitory effect on the same tumor cells, the extent was less than that of hIFN‐β gene.</description><subject>Antibodies - pharmacology</subject><subject>cationic multilamellar liposomes</subject><subject>Cell Division - physiology</subject><subject>colon cancer cells</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - secretion</subject><subject>Colonic Neoplasms - therapy</subject><subject>DNA, Complementary - administration & dosage</subject><subject>DNA, Complementary - genetics</subject><subject>Genetic Therapy - methods</subject><subject>Humans</subject><subject>IFN‐β cDNA</subject><subject>Interferon-beta - biosynthesis</subject><subject>Interferon-beta - genetics</subject><subject>Interferon-beta - secretion</subject><subject>Interferon-gamma - genetics</subject><subject>Liposomes</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>1521-6543</issn><issn>1039-9712</issn><issn>1521-6551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1OwzAUhC0EKqVwABZIXrEr-CexY4kNrcqPBGJT1lHqPNOgxA52ItQdR-AsHIRDcBJcteqGBV7YT56ZT3qD0CklF5Rk5JKmjIo0URkhjDDO2R4arv_GIk3p_m5O-CE6CuGVxCOJGqCBEoxLqYZoOTMGdIedxdrV67uwGjzWUNcBO4OXfVNYXNkOvAHv7M_H5_cXfgELGGzni7aFMsox11XOVho3fd1VddFEQOFxXbUuuAbCMTowRR3gZPuO0PPNbD69Gz883d5Prx_GmieJGgttUgqZWCQ0U5zTjHEmMrOQgoHRqVBcgSplWQqIAUlYCaJcCKlkQkqVUj5C5xtu691bD6HLmyqstyksuD7kUkVsxrJopBuj9i4EDyZvfdUUfpVTkq_bzf-0GzNnW3i_aKDcJbZ1Rv1qo79XNaz-B-bzyeNEppQwJbjiv7dih00</recordid><startdate>199805</startdate><enddate>199805</enddate><creator>Shimizu, Minoru</creator><creator>Akiyama, Seiji</creator><creator>Ito, Katsuki</creator><creator>Kasai, Yasushi</creator><creator>Takagi, Hiroshi</creator><creator>Kito, Mariko</creator><creator>Ohishi, Nobuko</creator><creator>Yagi, Kunio</creator><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199805</creationdate><title>Effect on colon cancer cells of human interferon‐β gene entrapped in cationic multilamellar liposomes</title><author>Shimizu, Minoru ; Akiyama, Seiji ; Ito, Katsuki ; Kasai, Yasushi ; Takagi, Hiroshi ; Kito, Mariko ; Ohishi, Nobuko ; Yagi, Kunio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3449-6cf51e86b4189331823268fb762efc56939e9d7dd6ec34702de6db679740d9513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Antibodies - pharmacology</topic><topic>cationic multilamellar liposomes</topic><topic>Cell Division - physiology</topic><topic>colon cancer cells</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - secretion</topic><topic>Colonic Neoplasms - therapy</topic><topic>DNA, Complementary - administration & dosage</topic><topic>DNA, Complementary - genetics</topic><topic>Genetic Therapy - methods</topic><topic>Humans</topic><topic>IFN‐β cDNA</topic><topic>Interferon-beta - biosynthesis</topic><topic>Interferon-beta - genetics</topic><topic>Interferon-beta - secretion</topic><topic>Interferon-gamma - genetics</topic><topic>Liposomes</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimizu, Minoru</creatorcontrib><creatorcontrib>Akiyama, Seiji</creatorcontrib><creatorcontrib>Ito, Katsuki</creatorcontrib><creatorcontrib>Kasai, Yasushi</creatorcontrib><creatorcontrib>Takagi, Hiroshi</creatorcontrib><creatorcontrib>Kito, Mariko</creatorcontrib><creatorcontrib>Ohishi, Nobuko</creatorcontrib><creatorcontrib>Yagi, Kunio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry and molecular biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimizu, Minoru</au><au>Akiyama, Seiji</au><au>Ito, Katsuki</au><au>Kasai, Yasushi</au><au>Takagi, Hiroshi</au><au>Kito, Mariko</au><au>Ohishi, Nobuko</au><au>Yagi, Kunio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect on colon cancer cells of human interferon‐β gene entrapped in cationic multilamellar liposomes</atitle><jtitle>Biochemistry and molecular biology international</jtitle><addtitle>Biochem Mol Biol Int</addtitle><date>1998-05</date><risdate>1998</risdate><volume>44</volume><issue>6</issue><spage>1235</spage><epage>1243</epage><pages>1235-1243</pages><issn>1521-6543</issn><issn>1039-9712</issn><eissn>1521-6551</eissn><abstract>When cultured cells of human colon cancer cell line SW480 were transfected with human interferon‐β (hIFN‐β) gene by means of cationic multilamellar liposomes, the endogenously produced hIFN‐β exhibited a remarkable anti‐proliferative effect on the cells, which was more effective than that of exogenously added hIFN‐β. This effect lasted for several days, and was blocked completely by the addition of sufficient amounts of anti‐hIFN‐β antibody. From experiments using a transwell plate and an infusion pump, we found that endogenously produced hIFN‐β acted effectively on the cells around the transfectants and that the growth‐inhibitory effect was totally retained upon continuous dilution of the medium. These data indicate that hIFN‐β expressed endogenously by transfer of its gene acted on these cancer cells mainly in a paracrine manner. Although the transfection with hIFN‐γ gene also revealed a definite growth‐inhibitory effect on the same tumor cells, the extent was less than that of hIFN‐β gene.</abstract><cop>UK</cop><pub>Informa Healthcare</pub><pmid>9623779</pmid><doi>10.1080/15216549800202332</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies - pharmacology cationic multilamellar liposomes Cell Division - physiology colon cancer cells Colonic Neoplasms - metabolism Colonic Neoplasms - secretion Colonic Neoplasms - therapy DNA, Complementary - administration & dosage DNA, Complementary - genetics Genetic Therapy - methods Humans IFN‐β cDNA Interferon-beta - biosynthesis Interferon-beta - genetics Interferon-beta - secretion Interferon-gamma - genetics Liposomes Transfection Tumor Cells, Cultured |
| title | Effect on colon cancer cells of human interferon‐β gene entrapped in cationic multilamellar liposomes |
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