Partially glucose-capped oligosaccharides are found on the hemoglobins of the deep-sea tube worm Riftia pachyptila
We report here the structural determination of N-linked oligosaccharides found on extracellular hemoglobins of the hydrothermal vent tube worm Riftia pachyptila. Structures were elucidated by a combination of electrospray ionization tandem mass spectrometry, matrix-assisted laser desorption/ionizati...
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| Veröffentlicht in: | Glycobiology (Oxford) 1998-07, Vol.8 (7), p.663-673 |
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| container_title | Glycobiology (Oxford) |
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| creator | Zal, Franck Küster, Bernhard Brian N., Green Harvey, David J. Lallier, François H. |
| description | We report here the structural determination of N-linked oligosaccharides found on extracellular hemoglobins of the hydrothermal vent tube worm Riftia pachyptila. Structures were elucidated by a combination of electrospray ionization tandem mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry, normal-phase high performance liquid chromatography, and exoglycosidase digestion. The sugar chains were found to consist mainly of high-mannosetype glycans with some structures partially capped by one or two terminal glucose residues. The present study represents the first report of the occurrence of glucose capping of Nlinked carbohydrates in a secreted glycoprotein of a metazoan. Previously, glucose capping has only been described for a membrane-bound surface glycoprotein from the unicellular parasite Leishmania mexicana amazonensis. |
| doi_str_mv | 10.1093/glycob/8.7.663 |
| format | Article |
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| fulltext | fulltext |
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| ispartof | Glycobiology (Oxford), 1998-07, Vol.8 (7), p.663-673 |
| issn | 0959-6658 1460-2423 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Carbohydrate Conformation Carbohydrate Sequence Chromatography, High Pressure Liquid Glucose - analysis glucose capping glycoprotein hemoglobin Hemoglobins - chemistry Hemoglobins - isolation & purification hydrothermal vent Leishmania mexicana Mass Spectrometry Membrane Glycoproteins - chemistry Models, Molecular Molecular Sequence Data Oligosaccharides - chemistry Oligosaccharides - isolation & purification Polychaeta Protozoan Proteins - chemistry Riftia pachyptila Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Partially glucose-capped oligosaccharides are found on the hemoglobins of the deep-sea tube worm Riftia pachyptila |
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