c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization
Expression of the c- fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c- fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyr...
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| Veröffentlicht in: | Brain research. Molecular brain research. 1990-06, Vol.8 (1), p.69-75 |
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| container_title | Brain research. Molecular brain research. |
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| creator | Hisanaga, Kinya Sagar, Stephen M. Hicks, Katy J. Swanson, Raymond A. Sharp, Frank R. |
| description | Expression of the c-
fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-
fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-
O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-
fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-
fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca
2+ uptake), higk K
+ and nerve growth factor had no detectable effect on c-
fos expression. These results show that depolarization does not induce c-
fos in astrocytes and suggest that c-
fos may play a role in differentiation and proliferation of astrocytes. |
| doi_str_mv | 10.1016/0169-328X(90)90011-2 |
| format | Article |
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fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-
fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-
O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-
fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-
fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca
2+ uptake), higk K
+ and nerve growth factor had no detectable effect on c-
fos expression. These results show that depolarization does not induce c-
fos in astrocytes and suggest that c-
fos may play a role in differentiation and proliferation of astrocytes.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/0169-328X(90)90011-2</identifier><identifier>PMID: 2166202</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Astrocyte culture ; Astrocytes - cytology ; Astrocytes - drug effects ; Astrocytes - metabolism ; c- fos ; Cell Differentiation ; Cell Division ; Cells, Cultured ; Cyclic AMP ; Epidermal growth factor ; Gene Expression Regulation - physiology ; Growth Substances - pharmacology ; Immunohistochemistry ; Mitogens - pharmacology ; Phorbol ester ; Protein kinase ; Proto-oncogene ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-fos ; Rats ; Rats, Inbred Strains ; RNA, Messenger - genetics ; RNA, Messenger - metabolism</subject><ispartof>Brain research. Molecular brain research., 1990-06, Vol.8 (1), p.69-75</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-aef4da0679308d721fdcbad1f0abf303154eb8b81e2f2548ba1a1ea1d2b14d9d3</citedby><cites>FETCH-LOGICAL-c303t-aef4da0679308d721fdcbad1f0abf303154eb8b81e2f2548ba1a1ea1d2b14d9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2166202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hisanaga, Kinya</creatorcontrib><creatorcontrib>Sagar, Stephen M.</creatorcontrib><creatorcontrib>Hicks, Katy J.</creatorcontrib><creatorcontrib>Swanson, Raymond A.</creatorcontrib><creatorcontrib>Sharp, Frank R.</creatorcontrib><title>c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>Expression of the c-
fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-
fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-
O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-
fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-
fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca
2+ uptake), higk K
+ and nerve growth factor had no detectable effect on c-
fos expression. These results show that depolarization does not induce c-
fos in astrocytes and suggest that c-
fos may play a role in differentiation and proliferation of astrocytes.</description><subject>Animals</subject><subject>Astrocyte culture</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>c- fos</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Cyclic AMP</subject><subject>Epidermal growth factor</subject><subject>Gene Expression Regulation - physiology</subject><subject>Growth Substances - pharmacology</subject><subject>Immunohistochemistry</subject><subject>Mitogens - pharmacology</subject><subject>Phorbol ester</subject><subject>Protein kinase</subject><subject>Proto-oncogene</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-fos</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLxDAUhYMo4_j4BwpdiS6quWmn02wEGXyB4EbBXUiTG410mjFJff1605nBpS5CwrnfPTecS8gB0FOgUJ2lw_OC1U_HnJ5wSgFytkHGUE9ZXvESNsn4F9kmOyG80gTVACMyYlBVjLIx6VVuXMgW3kWXu065Z-www8-FxxCs6zLbZTJE79RXxJCewSkrI-rsw8aXTFtj0GMXkzbQzg9WrU3iSmj6mHUuZhoXrpXefi_lPbJlZBtwf33vksery4fZTX53f307u7jLVUGLmEs0pZa0mvKC1nrKwGjVSA2GysYkAiYlNnVTAzLDJmXdSJCAEjRroNRcF7vkaOWbPvXWY4hiboPCtpUduj6IKedsUjD6LwiTmlUl8ASWK1B5F4JHIxbezqX_EkDFsBYxZC6GzAWnYrkWwVLb4dq_b-aof5vWe0j181UdUxrvFr0IymKnUFuPKgrt7N8DfgCMiaBV</recordid><startdate>199006</startdate><enddate>199006</enddate><creator>Hisanaga, Kinya</creator><creator>Sagar, Stephen M.</creator><creator>Hicks, Katy J.</creator><creator>Swanson, Raymond A.</creator><creator>Sharp, Frank R.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199006</creationdate><title>c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization</title><author>Hisanaga, Kinya ; Sagar, Stephen M. ; Hicks, Katy J. ; Swanson, Raymond A. ; Sharp, Frank R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-aef4da0679308d721fdcbad1f0abf303154eb8b81e2f2548ba1a1ea1d2b14d9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Astrocyte culture</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - metabolism</topic><topic>c- fos</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Cyclic AMP</topic><topic>Epidermal growth factor</topic><topic>Gene Expression Regulation - physiology</topic><topic>Growth Substances - pharmacology</topic><topic>Immunohistochemistry</topic><topic>Mitogens - pharmacology</topic><topic>Phorbol ester</topic><topic>Protein kinase</topic><topic>Proto-oncogene</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-fos</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hisanaga, Kinya</creatorcontrib><creatorcontrib>Sagar, Stephen M.</creatorcontrib><creatorcontrib>Hicks, Katy J.</creatorcontrib><creatorcontrib>Swanson, Raymond A.</creatorcontrib><creatorcontrib>Sharp, Frank R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hisanaga, Kinya</au><au>Sagar, Stephen M.</au><au>Hicks, Katy J.</au><au>Swanson, Raymond A.</au><au>Sharp, Frank R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>1990-06</date><risdate>1990</risdate><volume>8</volume><issue>1</issue><spage>69</spage><epage>75</epage><pages>69-75</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>Expression of the c-
fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-
fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-
O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-
fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-
fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca
2+ uptake), higk K
+ and nerve growth factor had no detectable effect on c-
fos expression. These results show that depolarization does not induce c-
fos in astrocytes and suggest that c-
fos may play a role in differentiation and proliferation of astrocytes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>2166202</pmid><doi>10.1016/0169-328X(90)90011-2</doi><tpages>7</tpages></addata></record> |
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| issn | 0169-328X 1872-6941 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Astrocyte culture Astrocytes - cytology Astrocytes - drug effects Astrocytes - metabolism c- fos Cell Differentiation Cell Division Cells, Cultured Cyclic AMP Epidermal growth factor Gene Expression Regulation - physiology Growth Substances - pharmacology Immunohistochemistry Mitogens - pharmacology Phorbol ester Protein kinase Proto-oncogene Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-fos Rats Rats, Inbred Strains RNA, Messenger - genetics RNA, Messenger - metabolism |
| title | c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization |
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