RNA lariat debranching enzyme as tool for analyzing RNA structure

The primary transcripts (pre-mRNAs) of most structural genes in higher eukaryotes contain intervening sequences (introns) that are removed by splicing. The lariat debranching enzyme is an RNA processing activity, originally identified in mammalian cell extracts, that specifically hydrolyzes the 2′-5...

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Veröffentlicht in:	Methods in Enzymology 1990, Vol.181, p.180-188
Hauptverfasser:	Ruskin, Barbara, Green, Michael R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genetic Techniques Miscellaneous Nucleic Acid Conformation Ribonucleases RNA - metabolism RNA - ultrastructure RNA Nucleotidyltransferases - metabolism RNA Precursors - genetics RNA Precursors - metabolism RNA Processing, Post-Transcriptional RNA Splicing
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