C-methylation occurs during the biosynthesis of heme d1
The biosynthetic origin of methyl groups in heme d1 isolated from the nitrite reductase cytochrome cd1 was investigated by a stable isotope labeling experiment. Pseudomonas aeruginosa (American Type Culture Collection strain 19429) was grown on a minimal medium supplemented with [13C]methionine. The...
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| Veröffentlicht in: | The Journal of biological chemistry 1990-08, Vol.265 (23), p.13498-13500 |
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| container_end_page | 13500 |
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| container_issue | 23 |
| container_start_page | 13498 |
| container_title | The Journal of biological chemistry |
| container_volume | 265 |
| creator | YAP-BONDOC, F BONDOC, L. L TIMKOVICH, R BAKER, D. C HEBBLER, A |
| description | The biosynthetic origin of methyl groups in heme d1 isolated from the nitrite reductase cytochrome cd1 was investigated by
a stable isotope labeling experiment. Pseudomonas aeruginosa (American Type Culture Collection strain 19429) was grown on
a minimal medium supplemented with [13C]methionine. The enzyme was purified, the heme extracted, converted into the free base
methyl ester derivative, and purified. 1H NMR and 13C NMR indicated that only the methyl groups attached to C2 and C7 are
derived from methionine. |
| doi_str_mv | 10.1016/S0021-9258(18)77374-2 |
| format | Article |
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a stable isotope labeling experiment. Pseudomonas aeruginosa (American Type Culture Collection strain 19429) was grown on
a minimal medium supplemented with [13C]methionine. The enzyme was purified, the heme extracted, converted into the free base
methyl ester derivative, and purified. 1H NMR and 13C NMR indicated that only the methyl groups attached to C2 and C7 are
derived from methionine.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)77374-2</identifier><identifier>PMID: 2166031</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Analytical, structural and metabolic biochemistry ; Bacterial Proteins ; Biological and medical sciences ; Carbon Isotopes ; Cytochrome c Group - metabolism ; Cytochromes - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Heme - analogs & derivatives ; Heme - biosynthesis ; Heterocyclic compounds, pigments ; Isotope Labeling - methods ; Magnetic Resonance Spectroscopy - methods ; Methionine - metabolism ; Methylation ; Miscellaneous ; Molecular Structure ; Nitrite Reductases ; Other biological molecules ; Pseudomonas aeruginosa - metabolism</subject><ispartof>The Journal of biological chemistry, 1990-08, Vol.265 (23), p.13498-13500</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-6db61745cb5b74ab392237d55c27e76213283c1c8522d015248cdee7334208173</citedby><cites>FETCH-LOGICAL-c410t-6db61745cb5b74ab392237d55c27e76213283c1c8522d015248cdee7334208173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19747957$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2166031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAP-BONDOC, F</creatorcontrib><creatorcontrib>BONDOC, L. L</creatorcontrib><creatorcontrib>TIMKOVICH, R</creatorcontrib><creatorcontrib>BAKER, D. C</creatorcontrib><creatorcontrib>HEBBLER, A</creatorcontrib><title>C-methylation occurs during the biosynthesis of heme d1</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The biosynthetic origin of methyl groups in heme d1 isolated from the nitrite reductase cytochrome cd1 was investigated by
a stable isotope labeling experiment. Pseudomonas aeruginosa (American Type Culture Collection strain 19429) was grown on
a minimal medium supplemented with [13C]methionine. The enzyme was purified, the heme extracted, converted into the free base
methyl ester derivative, and purified. 1H NMR and 13C NMR indicated that only the methyl groups attached to C2 and C7 are
derived from methionine.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Bacterial Proteins</subject><subject>Biological and medical sciences</subject><subject>Carbon Isotopes</subject><subject>Cytochrome c Group - metabolism</subject><subject>Cytochromes - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heme - analogs & derivatives</subject><subject>Heme - biosynthesis</subject><subject>Heterocyclic compounds, pigments</subject><subject>Isotope Labeling - methods</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Methionine - metabolism</subject><subject>Methylation</subject><subject>Miscellaneous</subject><subject>Molecular Structure</subject><subject>Nitrite Reductases</subject><subject>Other biological molecules</subject><subject>Pseudomonas aeruginosa - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFtLxDAQhYMo67r6Exb6oKIP1cwkadJHWbzBgg8q-BbaNLuN9LImLbL_3u6FdV5mYM45M3yETIHeAYXk_p1ShDhFoW5A3UrJJI_xiIyBKhYzAV_HZHyQnJKzEL7pUDyFERkhJAllMCZyFte2K9dV1rm2iVpjeh-ioveuWUZdaaPctWHdDFNwIWoXUWlrGxVwTk4WWRXsxb5PyOfT48fsJZ6_Pb_OHuax4UC7OCnyBCQXJhe55FnOUkQmCyEMSisTBIaKGTBKIBYUBHJlCmslYxypAskm5HqXu_LtT29Dp2sXjK2qrLFtH7RMU6SIYhCKndD4NgRvF3rlXZ35tQaqN8D0Fpje0NCg9BaYxsE33R_o89oWB9ee0LC_2u-zYLJq4bPGuPAfnkouU7F59HKnK92y_HXe6oGcGWhpTMRwRwPjqWJ_yuN8eQ</recordid><startdate>19900815</startdate><enddate>19900815</enddate><creator>YAP-BONDOC, F</creator><creator>BONDOC, L. L</creator><creator>TIMKOVICH, R</creator><creator>BAKER, D. C</creator><creator>HEBBLER, A</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900815</creationdate><title>C-methylation occurs during the biosynthesis of heme d1</title><author>YAP-BONDOC, F ; BONDOC, L. L ; TIMKOVICH, R ; BAKER, D. C ; HEBBLER, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-6db61745cb5b74ab392237d55c27e76213283c1c8522d015248cdee7334208173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Bacterial Proteins</topic><topic>Biological and medical sciences</topic><topic>Carbon Isotopes</topic><topic>Cytochrome c Group - metabolism</topic><topic>Cytochromes - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heme - analogs & derivatives</topic><topic>Heme - biosynthesis</topic><topic>Heterocyclic compounds, pigments</topic><topic>Isotope Labeling - methods</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>Methionine - metabolism</topic><topic>Methylation</topic><topic>Miscellaneous</topic><topic>Molecular Structure</topic><topic>Nitrite Reductases</topic><topic>Other biological molecules</topic><topic>Pseudomonas aeruginosa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAP-BONDOC, F</creatorcontrib><creatorcontrib>BONDOC, L. L</creatorcontrib><creatorcontrib>TIMKOVICH, R</creatorcontrib><creatorcontrib>BAKER, D. C</creatorcontrib><creatorcontrib>HEBBLER, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAP-BONDOC, F</au><au>BONDOC, L. L</au><au>TIMKOVICH, R</au><au>BAKER, D. C</au><au>HEBBLER, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C-methylation occurs during the biosynthesis of heme d1</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-08-15</date><risdate>1990</risdate><volume>265</volume><issue>23</issue><spage>13498</spage><epage>13500</epage><pages>13498-13500</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The biosynthetic origin of methyl groups in heme d1 isolated from the nitrite reductase cytochrome cd1 was investigated by
a stable isotope labeling experiment. Pseudomonas aeruginosa (American Type Culture Collection strain 19429) was grown on
a minimal medium supplemented with [13C]methionine. The enzyme was purified, the heme extracted, converted into the free base
methyl ester derivative, and purified. 1H NMR and 13C NMR indicated that only the methyl groups attached to C2 and C7 are
derived from methionine.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>2166031</pmid><doi>10.1016/S0021-9258(18)77374-2</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1990-08, Vol.265 (23), p.13498-13500 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79920225 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Bacterial Proteins Biological and medical sciences Carbon Isotopes Cytochrome c Group - metabolism Cytochromes - biosynthesis Fundamental and applied biological sciences. Psychology Heme - analogs & derivatives Heme - biosynthesis Heterocyclic compounds, pigments Isotope Labeling - methods Magnetic Resonance Spectroscopy - methods Methionine - metabolism Methylation Miscellaneous Molecular Structure Nitrite Reductases Other biological molecules Pseudomonas aeruginosa - metabolism |
| title | C-methylation occurs during the biosynthesis of heme d1 |
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