A mutation that converts serine340 of the HsdSK polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli K-12
A hybrid hsdS gene, encoding the HsdSts + d polypeptide, was constructed by joining the proximal region of the wild-type (wt) hsdS sequence with the distal region of the hsdSts + d sequence, at the hsdS BglII site. The hybrid hsdS-Sts + d gene exerts a trans-dominant effect on restriction and modifi...
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| Veröffentlicht in: | Gene 1990-05, Vol.90 (1), p.125-128 |
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| creator | Zinkevich, V E Weiserová, M Kryukov, V M Hubácek, J |
| description | A hybrid hsdS gene, encoding the HsdSts + d polypeptide, was constructed by joining the proximal region of the wild-type (wt) hsdS sequence with the distal region of the hsdSts + d sequence, at the hsdS BglII site. The hybrid hsdS-Sts + d gene exerts a trans-dominant effect on restriction and modification, which points to the location of the temperature-sensitive (ts) trans-dominant (+ d) mutation in the gene hsdSts + d distal region. Sequencing of the region downstream from the HindIII target in the Escherichia coli K-12 hsdSts + d mutant was carried out. It is identical to the wt hsdS sequence (GenBank/EMBL accession number ECHSDK LV00288), except for a single base-pair transition C1245---T. The results obtained support the idea that the trans-dominant effect of the ts mutation described earlier is related to the single base-pair transition in the nonhomologous region of the hsdSts + d sequence. |
| doi_str_mv | 10.1016/0378-1119(90)90447-Y |
| format | Article |
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The hybrid hsdS-Sts + d gene exerts a trans-dominant effect on restriction and modification, which points to the location of the temperature-sensitive (ts) trans-dominant (+ d) mutation in the gene hsdSts + d distal region. Sequencing of the region downstream from the HindIII target in the Escherichia coli K-12 hsdSts + d mutant was carried out. It is identical to the wt hsdS sequence (GenBank/EMBL accession number ECHSDK LV00288), except for a single base-pair transition C1245---T. The results obtained support the idea that the trans-dominant effect of the ts mutation described earlier is related to the single base-pair transition in the nonhomologous region of the hsdSts + d sequence.</description><identifier>ISSN: 0378-1119</identifier><identifier>DOI: 10.1016/0378-1119(90)90447-Y</identifier><identifier>PMID: 2116365</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Amino Acid Sequence ; Base Sequence ; Cloning, Molecular ; DNA Mutational Analysis ; DNA Restriction-Modification Enzymes - genetics ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Molecular Sequence Data ; Phenotype ; Phenylalanine ; Recombinant Proteins ; Restriction Mapping ; Serine ; Structure-Activity Relationship</subject><ispartof>Gene, 1990-05, Vol.90 (1), p.125-128</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1495-1887fb798add3a74cf6be3a2775d0202ac11713a6d40a7afea58de3f464dbccf3</citedby><cites>FETCH-LOGICAL-c1495-1887fb798add3a74cf6be3a2775d0202ac11713a6d40a7afea58de3f464dbccf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2116365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zinkevich, V E</creatorcontrib><creatorcontrib>Weiserová, M</creatorcontrib><creatorcontrib>Kryukov, V M</creatorcontrib><creatorcontrib>Hubácek, J</creatorcontrib><title>A mutation that converts serine340 of the HsdSK polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli K-12</title><title>Gene</title><addtitle>Gene</addtitle><description>A hybrid hsdS gene, encoding the HsdSts + d polypeptide, was constructed by joining the proximal region of the wild-type (wt) hsdS sequence with the distal region of the hsdSts + d sequence, at the hsdS BglII site. The hybrid hsdS-Sts + d gene exerts a trans-dominant effect on restriction and modification, which points to the location of the temperature-sensitive (ts) trans-dominant (+ d) mutation in the gene hsdSts + d distal region. Sequencing of the region downstream from the HindIII target in the Escherichia coli K-12 hsdSts + d mutant was carried out. It is identical to the wt hsdS sequence (GenBank/EMBL accession number ECHSDK LV00288), except for a single base-pair transition C1245---T. The results obtained support the idea that the trans-dominant effect of the ts mutation described earlier is related to the single base-pair transition in the nonhomologous region of the hsdSts + d sequence.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA Mutational Analysis</subject><subject>DNA Restriction-Modification Enzymes - genetics</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Molecular Sequence Data</subject><subject>Phenotype</subject><subject>Phenylalanine</subject><subject>Recombinant Proteins</subject><subject>Restriction Mapping</subject><subject>Serine</subject><subject>Structure-Activity Relationship</subject><issn>0378-1119</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UUtPxCAQ5qDx_Q804WT0UIVCSzluzPqIJh7UgyfCwpDFtKVC12R_iP9X6m52LpPMfA_4BqFzSm4oofUtYaIpKKXySpJrSTgXxeceOtqND9FxSl8kV1WVB-igpLRmdXWEfme4W4169KHH41KP2IT-B-KYcILoe2Cc4ODyCvBjsm_PeAjteoBh9BbwGPCwhH7d6lb3GYx1b7HPXHAOTO5ZNEIaozf_BtO6C9Y7bzaOvsfzZJbZySy9zt6tx88FLU_RvtNtgrNtP0Ef9_P3u8fi5fXh6W72UhjKZVXQphFuIWSjrWVacOPqBTBdClFZUpJSG0oFZbq2nGihHeiqscAcr7ldGOPYCbrc6A4xfK_yS1Xnk4E2fwfCKikhJa0aITOQb4AmhpQiODVE3-m4VpSo6QJqilpNUStJ1P8F1GemXWz1V4sO7I60jZ_9Af6hhec</recordid><startdate>19900531</startdate><enddate>19900531</enddate><creator>Zinkevich, V E</creator><creator>Weiserová, M</creator><creator>Kryukov, V M</creator><creator>Hubácek, J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900531</creationdate><title>A mutation that converts serine340 of the HsdSK polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli K-12</title><author>Zinkevich, V E ; Weiserová, M ; Kryukov, V M ; Hubácek, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1495-1887fb798add3a74cf6be3a2775d0202ac11713a6d40a7afea58de3f464dbccf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA Mutational Analysis</topic><topic>DNA Restriction-Modification Enzymes - genetics</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Molecular Sequence Data</topic><topic>Phenotype</topic><topic>Phenylalanine</topic><topic>Recombinant Proteins</topic><topic>Restriction Mapping</topic><topic>Serine</topic><topic>Structure-Activity Relationship</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zinkevich, V E</creatorcontrib><creatorcontrib>Weiserová, M</creatorcontrib><creatorcontrib>Kryukov, V M</creatorcontrib><creatorcontrib>Hubácek, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zinkevich, V E</au><au>Weiserová, M</au><au>Kryukov, V M</au><au>Hubácek, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A mutation that converts serine340 of the HsdSK polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli K-12</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1990-05-31</date><risdate>1990</risdate><volume>90</volume><issue>1</issue><spage>125</spage><epage>128</epage><pages>125-128</pages><issn>0378-1119</issn><abstract>A hybrid hsdS gene, encoding the HsdSts + d polypeptide, was constructed by joining the proximal region of the wild-type (wt) hsdS sequence with the distal region of the hsdSts + d sequence, at the hsdS BglII site. The hybrid hsdS-Sts + d gene exerts a trans-dominant effect on restriction and modification, which points to the location of the temperature-sensitive (ts) trans-dominant (+ d) mutation in the gene hsdSts + d distal region. Sequencing of the region downstream from the HindIII target in the Escherichia coli K-12 hsdSts + d mutant was carried out. It is identical to the wt hsdS sequence (GenBank/EMBL accession number ECHSDK LV00288), except for a single base-pair transition C1245---T. The results obtained support the idea that the trans-dominant effect of the ts mutation described earlier is related to the single base-pair transition in the nonhomologous region of the hsdSts + d sequence.</abstract><cop>Netherlands</cop><pmid>2116365</pmid><doi>10.1016/0378-1119(90)90447-Y</doi><tpages>4</tpages></addata></record> |
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| ispartof | Gene, 1990-05, Vol.90 (1), p.125-128 |
| issn | 0378-1119 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79915879 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Base Sequence Cloning, Molecular DNA Mutational Analysis DNA Restriction-Modification Enzymes - genetics Escherichia coli - enzymology Escherichia coli - genetics Molecular Sequence Data Phenotype Phenylalanine Recombinant Proteins Restriction Mapping Serine Structure-Activity Relationship |
| title | A mutation that converts serine340 of the HsdSK polypeptide to phenylalanine and its effects on restriction and modification in Escherichia coli K-12 |
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