Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585
We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wi...
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| Veröffentlicht in: | Applied microbiology and biotechnology 1990-02, Vol.32 (5), p.560-567 |
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| container_title | Applied microbiology and biotechnology |
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| creator | PIRET, J RESENDIZ, B MAHRO, B JIN-YOU ZHANG SERPE, E ROMERO, J CONNORS, N DEMAIN, A. L |
| description | We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities to this mutant. The recombinant plasmid exhibits a variable copy number in different transformants. Hybridization experiments indicate that sequences homologous to the cloned region are present in various beta-lactam-producing Streptomyces spp. but absent in species which are not known to produce this class of antibiotics. Furthermore, the chromosomal copy of the cloned region lies in close proximity to a gene coding for the isopenicillin N synthase gene of the cephalosphorin pathway. |
| doi_str_mv | 10.1007/BF00173728 |
| format | Article |
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L</creator><creatorcontrib>PIRET, J ; RESENDIZ, B ; MAHRO, B ; JIN-YOU ZHANG ; SERPE, E ; ROMERO, J ; CONNORS, N ; DEMAIN, A. L</creatorcontrib><description>We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities to this mutant. The recombinant plasmid exhibits a variable copy number in different transformants. Hybridization experiments indicate that sequences homologous to the cloned region are present in various beta-lactam-producing Streptomyces spp. but absent in species which are not known to produce this class of antibiotics. Furthermore, the chromosomal copy of the cloned region lies in close proximity to a gene coding for the isopenicillin N synthase gene of the cephalosphorin pathway.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/BF00173728</identifier><identifier>PMID: 1366440</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; cephalosporins ; Cephalosporins - biosynthesis ; cloning ; Cloning, Molecular ; complementation ; DNA ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial - genetics ; Genetic Complementation Test ; hybridization analysis ; Metabolism. Enzymes ; Microbiology ; Mission oriented research ; mutants ; Mutation ; nucleotide sequence ; Oxidoreductases - genetics ; Peptide Synthases - genetics ; Phenotype ; Physiology and metabolism ; Plasmids - genetics ; Restriction Mapping ; Streptomyces - enzymology ; Streptomyces - genetics ; Streptomyces - metabolism ; Transformation, Bacterial</subject><ispartof>Applied microbiology and biotechnology, 1990-02, Vol.32 (5), p.560-567</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-5dde38182ef5d72a93b98763b9a2fb6a07dfe5d00c3d90e80840910715b85313</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6871863$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1366440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PIRET, J</creatorcontrib><creatorcontrib>RESENDIZ, B</creatorcontrib><creatorcontrib>MAHRO, B</creatorcontrib><creatorcontrib>JIN-YOU ZHANG</creatorcontrib><creatorcontrib>SERPE, E</creatorcontrib><creatorcontrib>ROMERO, J</creatorcontrib><creatorcontrib>CONNORS, N</creatorcontrib><creatorcontrib>DEMAIN, A. L</creatorcontrib><title>Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities to this mutant. The recombinant plasmid exhibits a variable copy number in different transformants. Hybridization experiments indicate that sequences homologous to the cloned region are present in various beta-lactam-producing Streptomyces spp. but absent in species which are not known to produce this class of antibiotics. Furthermore, the chromosomal copy of the cloned region lies in close proximity to a gene coding for the isopenicillin N synthase gene of the cephalosphorin pathway.</description><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>cephalosporins</subject><subject>Cephalosporins - biosynthesis</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>complementation</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial - genetics</subject><subject>Genetic Complementation Test</subject><subject>hybridization analysis</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>mutants</subject><subject>Mutation</subject><subject>nucleotide sequence</subject><subject>Oxidoreductases - genetics</subject><subject>Peptide Synthases - genetics</subject><subject>Phenotype</subject><subject>Physiology and metabolism</subject><subject>Plasmids - genetics</subject><subject>Restriction Mapping</subject><subject>Streptomyces - enzymology</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Transformation, Bacterial</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1r20AQBuAltLiO20vuBR1KDwE1u1rth46NqZOCScDxXYx3R_EGSavuSoHk12eDTXzsZQZmHt7DDCEXjP5ilKqr6xWlTHFV6DMyZyUvcipZ-YnM01TkSlT6CzmP8SmpQks5IzPGpSxLOid-uYcAZsTgXmF0vs-gt5nx3dBih_14mPkmg8zgsIfWx8EH1-cWG2dcElk3jZBaMg9jwGH03YvBmJkWnqfWPWKYYna32awzLrT4Sj430Eb8duwLsl392S5v8_X9zd_l73VueFmMubAWuWa6wEZYVUDFd5VWMlUomp0EqmyDwlJquK0oaqpLWjGqmNhpwRlfkJ-H2CH4fxPGse5cNNi20KOfYq2qKp1C8_9CJlOqTnJBLg_QBB9jwKYegusgvNSM1u9fqE9fSPj7MXXadWhP9HD2tP9x3EM00DYBeuPiB5NaMS05fwMp-Y68</recordid><startdate>19900201</startdate><enddate>19900201</enddate><creator>PIRET, J</creator><creator>RESENDIZ, B</creator><creator>MAHRO, B</creator><creator>JIN-YOU ZHANG</creator><creator>SERPE, E</creator><creator>ROMERO, J</creator><creator>CONNORS, N</creator><creator>DEMAIN, A. L</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900201</creationdate><title>Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585</title><author>PIRET, J ; RESENDIZ, B ; MAHRO, B ; JIN-YOU ZHANG ; SERPE, E ; ROMERO, J ; CONNORS, N ; DEMAIN, A. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-5dde38182ef5d72a93b98763b9a2fb6a07dfe5d00c3d90e80840910715b85313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>cephalosporins</topic><topic>Cephalosporins - biosynthesis</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>complementation</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial - genetics</topic><topic>Genetic Complementation Test</topic><topic>hybridization analysis</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>mutants</topic><topic>Mutation</topic><topic>nucleotide sequence</topic><topic>Oxidoreductases - genetics</topic><topic>Peptide Synthases - genetics</topic><topic>Phenotype</topic><topic>Physiology and metabolism</topic><topic>Plasmids - genetics</topic><topic>Restriction Mapping</topic><topic>Streptomyces - enzymology</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Transformation, Bacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PIRET, J</creatorcontrib><creatorcontrib>RESENDIZ, B</creatorcontrib><creatorcontrib>MAHRO, B</creatorcontrib><creatorcontrib>JIN-YOU ZHANG</creatorcontrib><creatorcontrib>SERPE, E</creatorcontrib><creatorcontrib>ROMERO, J</creatorcontrib><creatorcontrib>CONNORS, N</creatorcontrib><creatorcontrib>DEMAIN, A. 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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>1990-02-01</date><risdate>1990</risdate><volume>32</volume><issue>5</issue><spage>560</spage><epage>567</epage><pages>560-567</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities to this mutant. The recombinant plasmid exhibits a variable copy number in different transformants. Hybridization experiments indicate that sequences homologous to the cloned region are present in various beta-lactam-producing Streptomyces spp. but absent in species which are not known to produce this class of antibiotics. Furthermore, the chromosomal copy of the cloned region lies in close proximity to a gene coding for the isopenicillin N synthase gene of the cephalosphorin pathway.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>1366440</pmid><doi>10.1007/BF00173728</doi><tpages>8</tpages></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 1990-02, Vol.32 (5), p.560-567 |
| issn | 0175-7598 1432-0614 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology cephalosporins Cephalosporins - biosynthesis cloning Cloning, Molecular complementation DNA DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - genetics Genetic Complementation Test hybridization analysis Metabolism. Enzymes Microbiology Mission oriented research mutants Mutation nucleotide sequence Oxidoreductases - genetics Peptide Synthases - genetics Phenotype Physiology and metabolism Plasmids - genetics Restriction Mapping Streptomyces - enzymology Streptomyces - genetics Streptomyces - metabolism Transformation, Bacterial |
| title | Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585 |
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