Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds
A lectin was isolated from the saline extract of Artocarpus incisa seed by affinity chromatography on cross-linked Adenanthera pavonina galactomannan in 0.15 M NaCl. The lectin was also retained in a d-galagarose resin and had no requirements for divalent metal cations (Ca 2+ and Mn 2+) for activity...
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| Veröffentlicht in: | Phytochemistry (Oxford) 1998-04, Vol.47 (7), p.1183-1188 |
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| container_title | Phytochemistry (Oxford) |
| container_volume | 47 |
| creator | Moreira, Renato A. Castelo-Branco, Carlos C. Monteiro, Ana C.O. Tavares, Ricardo O. Beltramini, Leila M. |
| description | A lectin was isolated from the saline extract of
Artocarpus incisa seed by affinity chromatography on cross-linked
Adenanthera pavonina galactomannan in 0.15 M NaCl. The lectin was also retained in a
d-galagarose resin and had no requirements for divalent metal cations (Ca
2+ and Mn
2+) for activity. The lectin contains 2.1% of carbohydrate and is characterized by high contents of acidic and hydroxylated amino acids. The lectin presented two protein bands in SDS-PAGE, with
M
r
15.5 and 12 kDa, respectively, and contains no α-helix, 64% antiparallel β-sheet and 21% parallel β-sheet/β-turn. When submitted to gel filtration in Superose 12 R (FPLC) and Superdex 75 HR
5
5
(HPLC) columns, the lectin showed an
M
r
of 48–49 kDa, suggesting a tetrameric structure. |
| doi_str_mv | 10.1016/S0031-9422(97)00753-X |
| format | Article |
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Artocarpus incisa seed by affinity chromatography on cross-linked
Adenanthera pavonina galactomannan in 0.15 M NaCl. The lectin was also retained in a
d-galagarose resin and had no requirements for divalent metal cations (Ca
2+ and Mn
2+) for activity. The lectin contains 2.1% of carbohydrate and is characterized by high contents of acidic and hydroxylated amino acids. The lectin presented two protein bands in SDS-PAGE, with
M
r
15.5 and 12 kDa, respectively, and contains no α-helix, 64% antiparallel β-sheet and 21% parallel β-sheet/β-turn. When submitted to gel filtration in Superose 12 R (FPLC) and Superdex 75 HR
5
5
(HPLC) columns, the lectin showed an
M
r
of 48–49 kDa, suggesting a tetrameric structure.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/S0031-9422(97)00753-X</identifier><identifier>PMID: 9611823</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Calcium - chemistry ; Chemical constitution ; Chromatography, Affinity ; Chromatography, Gel ; Chromatography, Ion Exchange ; Circular Dichroism ; Electrophoresis, Polyacrylamide Gel ; Erythrocytes - drug effects ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; Hemagglutination Tests ; Humans ; Isoelectric Focusing ; Lectins - chemistry ; Lectins - isolation & purification ; Lectins - pharmacology ; Manganese - chemistry ; Plant Lectins ; Plant physiology and development ; Plants - chemistry ; Protein Structure, Secondary ; Proteins ; Seeds - chemistry</subject><ispartof>Phytochemistry (Oxford), 1998-04, Vol.47 (7), p.1183-1188</ispartof><rights>1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-52f721f4797ff0c98ccfdc5a10ebf5f5f9b3790df1a46d9e87bbc184caaffccd3</citedby><cites>FETCH-LOGICAL-c455t-52f721f4797ff0c98ccfdc5a10ebf5f5f9b3790df1a46d9e87bbc184caaffccd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0031-9422(97)00753-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2243949$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9611823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moreira, Renato A.</creatorcontrib><creatorcontrib>Castelo-Branco, Carlos C.</creatorcontrib><creatorcontrib>Monteiro, Ana C.O.</creatorcontrib><creatorcontrib>Tavares, Ricardo O.</creatorcontrib><creatorcontrib>Beltramini, Leila M.</creatorcontrib><title>Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>A lectin was isolated from the saline extract of
Artocarpus incisa seed by affinity chromatography on cross-linked
Adenanthera pavonina galactomannan in 0.15 M NaCl. The lectin was also retained in a
d-galagarose resin and had no requirements for divalent metal cations (Ca
2+ and Mn
2+) for activity. The lectin contains 2.1% of carbohydrate and is characterized by high contents of acidic and hydroxylated amino acids. The lectin presented two protein bands in SDS-PAGE, with
M
r
15.5 and 12 kDa, respectively, and contains no α-helix, 64% antiparallel β-sheet and 21% parallel β-sheet/β-turn. When submitted to gel filtration in Superose 12 R (FPLC) and Superdex 75 HR
5
5
(HPLC) columns, the lectin showed an
M
r
of 48–49 kDa, suggesting a tetrameric structure.</description><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Calcium - chemistry</subject><subject>Chemical constitution</subject><subject>Chromatography, Affinity</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange</subject><subject>Circular Dichroism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Erythrocytes - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>Hemagglutination Tests</subject><subject>Humans</subject><subject>Isoelectric Focusing</subject><subject>Lectins - chemistry</subject><subject>Lectins - isolation & purification</subject><subject>Lectins - pharmacology</subject><subject>Manganese - chemistry</subject><subject>Plant Lectins</subject><subject>Plant physiology and development</subject><subject>Plants - chemistry</subject><subject>Protein Structure, Secondary</subject><subject>Proteins</subject><subject>Seeds - chemistry</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMoOo4-gpCFiC6qSds0zUoG8QYDghdwF05PEox0mjHpCPr0di7MVs7iLP7vXPgIOeHskjNeXb0wVvBMlXl-ruQFY1IU2fsOGfFaFlkhGdsloy1yQA5T-mSMCVFV-2RfVZzXeTEiz48ptND70FHoDJ1D7D20FD8gAvY2-t91GBwF2lrsfUddDDM6iX1AiPNFor5Dn4BOL2my1qQjsuegTfZ408fk7e729eYhmz7dP95MphmWQvSZyJ3MuSulks4xVDWiMyiAM9s4MZRqCqmYcRzKyihby6ZBXpcI4ByiKcbkbL13HsPXwqZez3xC27bQ2bBIWirFBhdyAMUaxBhSitbpefQziD-aM710qVcu9VKUVlKvXOr3Ye5kc2DRzKzZTm3kDfnpJoeE0LoISxFbLM_LQpVqwK7XmB1kfHsbdUJvO7TGx0GoNsH_88gf2ZeSJQ</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>Moreira, Renato A.</creator><creator>Castelo-Branco, Carlos C.</creator><creator>Monteiro, Ana C.O.</creator><creator>Tavares, Ricardo O.</creator><creator>Beltramini, Leila M.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980401</creationdate><title>Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds</title><author>Moreira, Renato A. ; Castelo-Branco, Carlos C. ; Monteiro, Ana C.O. ; Tavares, Ricardo O. ; Beltramini, Leila M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-52f721f4797ff0c98ccfdc5a10ebf5f5f9b3790df1a46d9e87bbc184caaffccd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Calcium - chemistry</topic><topic>Chemical constitution</topic><topic>Chromatography, Affinity</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Ion Exchange</topic><topic>Circular Dichroism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Erythrocytes - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins</topic><topic>Hemagglutination Tests</topic><topic>Humans</topic><topic>Isoelectric Focusing</topic><topic>Lectins - chemistry</topic><topic>Lectins - isolation & purification</topic><topic>Lectins - pharmacology</topic><topic>Manganese - chemistry</topic><topic>Plant Lectins</topic><topic>Plant physiology and development</topic><topic>Plants - chemistry</topic><topic>Protein Structure, Secondary</topic><topic>Proteins</topic><topic>Seeds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moreira, Renato A.</creatorcontrib><creatorcontrib>Castelo-Branco, Carlos C.</creatorcontrib><creatorcontrib>Monteiro, Ana C.O.</creatorcontrib><creatorcontrib>Tavares, Ricardo O.</creatorcontrib><creatorcontrib>Beltramini, Leila M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moreira, Renato A.</au><au>Castelo-Branco, Carlos C.</au><au>Monteiro, Ana C.O.</au><au>Tavares, Ricardo O.</au><au>Beltramini, Leila M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>47</volume><issue>7</issue><spage>1183</spage><epage>1188</epage><pages>1183-1188</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>A lectin was isolated from the saline extract of
Artocarpus incisa seed by affinity chromatography on cross-linked
Adenanthera pavonina galactomannan in 0.15 M NaCl. The lectin was also retained in a
d-galagarose resin and had no requirements for divalent metal cations (Ca
2+ and Mn
2+) for activity. The lectin contains 2.1% of carbohydrate and is characterized by high contents of acidic and hydroxylated amino acids. The lectin presented two protein bands in SDS-PAGE, with
M
r
15.5 and 12 kDa, respectively, and contains no α-helix, 64% antiparallel β-sheet and 21% parallel β-sheet/β-turn. When submitted to gel filtration in Superose 12 R (FPLC) and Superdex 75 HR
5
5
(HPLC) columns, the lectin showed an
M
r
of 48–49 kDa, suggesting a tetrameric structure.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>9611823</pmid><doi>10.1016/S0031-9422(97)00753-X</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-9422 |
| ispartof | Phytochemistry (Oxford), 1998-04, Vol.47 (7), p.1183-1188 |
| issn | 0031-9422 1873-3700 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79908737 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Amino Acids - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Calcium - chemistry Chemical constitution Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Circular Dichroism Electrophoresis, Polyacrylamide Gel Erythrocytes - drug effects Fundamental and applied biological sciences. Psychology Glycoproteins Hemagglutination Tests Humans Isoelectric Focusing Lectins - chemistry Lectins - isolation & purification Lectins - pharmacology Manganese - chemistry Plant Lectins Plant physiology and development Plants - chemistry Protein Structure, Secondary Proteins Seeds - chemistry |
| title | Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds |
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