The phosphate carrier from yeast mitochondria: dimerization is a prerequisite for function

Wild type phosphate carrier (PIC) from Saccharomyces cerevisiae and recombinant PIC proteins with different C-terminal extensions were expressed in Escherichia coli as inclusion bodies. From these, PIC was isolated with the detergent sodium lauroyl sarcosinate in a form, partially monomeric and unfo...

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Veröffentlicht in:	The Journal of biological chemistry 1998-06, Vol.273 (23), p.14269-14276
Hauptverfasser:	Schroers, A, Burkovski, A, Wohlrab, H, Kramer, R
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVE TRANSPORT ANION ANIONES ANIONS BINDING Biological Transport - physiology Carrier Proteins - chemistry CELL MEMBRANES Detergents - pharmacology Dimerization Escherichia coli Escherichia coli - genetics Ethylmaleimide - pharmacology FOSFATOS Fungal Proteins - chemistry Kinetics Liposomes - metabolism MEMBRANAS CELULARES MEMBRANE CELLULAIRE Membrane Proteins - physiology MITOCHONDRIA Mitochondria - physiology MITOCHONDRIE MITOCONDRIA PHOSPHATE phosphate carrier PHOSPHATE TRANSPORT PROTEIN Phosphate-Binding Proteins PHOSPHATES Phosphates - metabolism Phospholipids - metabolism Pic protein Protein Conformation PROTEINAS PROTEINAS RECOMBINANTES PROTEINE PROTEINE RECOMBINANTE PROTEINS RECOMBINANT PROTEINS Recombinant Proteins - chemistry SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - physiology
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