Pharmacological characterisation of the recombinant human CRF binding protein using a simple assay

We present the pharmacological characterisation of the recombinant human corticotropin releasing factor binding protein (hCRF-BP) using a simple assay. In this assay we employed [ 3H]urocortin as the radioligand and, as a means to separate bound and free ligand, adsorption to activated charcoal. Usi...

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Veröffentlicht in:	Journal of neuroscience methods 1998-03, Vol.80 (1), p.99-105
Hauptverfasser:	Ardati, Ali, Gottowik, Juergen, Henriot, Stephane, Clerc, Roger G, Kilpatrick, Gavin J
Format:	Artikel
Sprache:	eng
Schlagworte:	[ 3H]Urocortin Binding, Competitive Biological and medical sciences Carrier Proteins - metabolism Carrier Proteins - pharmacology Corticotropin-Releasing Hormone - metabolism CRF CRF binding protein CRH Cross-Linking Reagents Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Humans Kinetics Radioligand Assay - methods Recombinant Proteins - metabolism Recombinant Proteins - pharmacology Sodium Dodecyl Sulfate Tritium - metabolism Urocortins Vertebrates: nervous system and sense organs
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