Winged helix hepatocyte nuclear factor 3 and POU-domain protein Brn-2/N-Oct-3 bind overlapping sites on the neuronal promoter of human aromatic l-amino acid decarboxylase gene
The neuronal promoter of human aromatic l-amino acid decarboxylase gene has been analysed to elucidate the mechanisms of neuron type-specific expression. The (−560/+92) promoter segment was sufficient to direct luciferase expression at a higher level in SK-N-BE neuroblastoma cells, than in CHP126 ne...
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| Veröffentlicht in: | Brain research. Molecular brain research. 1998-05, Vol.56 (1), p.227-237 |
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| creator | Raynal, Jean-François Dugast, Claire Le Van Thaı̈, Agathe Weber, Michel J. |
| description | The neuronal promoter of human aromatic
l-amino acid decarboxylase gene has been analysed to elucidate the mechanisms of neuron type-specific expression. The (−560/+92) promoter segment was sufficient to direct luciferase expression at a higher level in SK-N-BE neuroblastoma cells, than in CHP126 neuroepithelia, HepG2 hepatoma or SK-Hep1 epithelioma cells. Deletions experiments showed that this segment contained a neuronal-specific (element T1) and a SK-N-BE-specific (element N1)
cis-activating sequences. Element T1 (−72/−36) bound Sp1 and NF-Y proteins, and unidentified neuronal-specific factors. Element N1 (−102/−72) bound cell-specific factors, identified as HNF-3, N-Oct-3/Brn-2 and N-Oct-2. HNF-3 proteins recognized the sequence TCAGTAAATA that matches the consensus motif. Oct-1, N-Oct-2 and N-Oct-3 bound the AAATAATGC sequence that overlaps the HNF-3 binding site. In addition, we show that the HNF-3 binding sites from aldolase C and
HNF-3β gene promoters also bind N-Oct-2 and N-Oct-3 proteins. These data suggest a functional interplay of winged helix/forkhead and POU-domain transcription factors on a variety of neuronal gene promoters. |
| doi_str_mv | 10.1016/S0169-328X(98)00048-5 |
| format | Article |
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l-amino acid decarboxylase gene has been analysed to elucidate the mechanisms of neuron type-specific expression. The (−560/+92) promoter segment was sufficient to direct luciferase expression at a higher level in SK-N-BE neuroblastoma cells, than in CHP126 neuroepithelia, HepG2 hepatoma or SK-Hep1 epithelioma cells. Deletions experiments showed that this segment contained a neuronal-specific (element T1) and a SK-N-BE-specific (element N1)
cis-activating sequences. Element T1 (−72/−36) bound Sp1 and NF-Y proteins, and unidentified neuronal-specific factors. Element N1 (−102/−72) bound cell-specific factors, identified as HNF-3, N-Oct-3/Brn-2 and N-Oct-2. HNF-3 proteins recognized the sequence TCAGTAAATA that matches the consensus motif. Oct-1, N-Oct-2 and N-Oct-3 bound the AAATAATGC sequence that overlaps the HNF-3 binding site. In addition, we show that the HNF-3 binding sites from aldolase C and
HNF-3β gene promoters also bind N-Oct-2 and N-Oct-3 proteins. These data suggest a functional interplay of winged helix/forkhead and POU-domain transcription factors on a variety of neuronal gene promoters.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/S0169-328X(98)00048-5</identifier><identifier>PMID: 9602135</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Aromatic-L-Amino-Acid Decarboxylases - genetics ; Binding Sites - genetics ; Biological and medical sciences ; COS Cells ; DNA-Binding Proteins - metabolism ; DNA-Binding Proteins - physiology ; DOPA decarboxylase ; Dopamine ; Drifter ; Fundamental and applied biological sciences. Psychology ; Gene regulation ; Helix-Loop-Helix Motifs ; Hepatocyte Nuclear Factor 3-alpha ; Hepatocyte Nuclear Factor 3-beta ; Hepatocyte Nuclear Factor 3-gamma ; Homeodomain Proteins ; Humans ; Molecular and cellular biology ; Molecular genetics ; Neurons - cytology ; Neurons - metabolism ; NF-Y ; Nuclear Proteins - metabolism ; Nuclear Proteins - physiology ; POU Domain Factors ; Promoter Regions, Genetic - genetics ; Sp1 ; Transcription Factors - metabolism ; Transcription Factors - physiology ; Transcription. Transcription factor. Splicing. Rna processing ; Tumor Cells, Cultured</subject><ispartof>Brain research. Molecular brain research., 1998-05, Vol.56 (1), p.227-237</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><rights>Copyright 1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-1a811af388d533a0850d6b0cc0b2ddc1a986e3893be8604238d7fd40e5be18e73</citedby><cites>FETCH-LOGICAL-c352t-1a811af388d533a0850d6b0cc0b2ddc1a986e3893be8604238d7fd40e5be18e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2396677$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9602135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raynal, Jean-François</creatorcontrib><creatorcontrib>Dugast, Claire</creatorcontrib><creatorcontrib>Le Van Thaı̈, Agathe</creatorcontrib><creatorcontrib>Weber, Michel J.</creatorcontrib><title>Winged helix hepatocyte nuclear factor 3 and POU-domain protein Brn-2/N-Oct-3 bind overlapping sites on the neuronal promoter of human aromatic l-amino acid decarboxylase gene</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>The neuronal promoter of human aromatic
l-amino acid decarboxylase gene has been analysed to elucidate the mechanisms of neuron type-specific expression. The (−560/+92) promoter segment was sufficient to direct luciferase expression at a higher level in SK-N-BE neuroblastoma cells, than in CHP126 neuroepithelia, HepG2 hepatoma or SK-Hep1 epithelioma cells. Deletions experiments showed that this segment contained a neuronal-specific (element T1) and a SK-N-BE-specific (element N1)
cis-activating sequences. Element T1 (−72/−36) bound Sp1 and NF-Y proteins, and unidentified neuronal-specific factors. Element N1 (−102/−72) bound cell-specific factors, identified as HNF-3, N-Oct-3/Brn-2 and N-Oct-2. HNF-3 proteins recognized the sequence TCAGTAAATA that matches the consensus motif. Oct-1, N-Oct-2 and N-Oct-3 bound the AAATAATGC sequence that overlaps the HNF-3 binding site. In addition, we show that the HNF-3 binding sites from aldolase C and
HNF-3β gene promoters also bind N-Oct-2 and N-Oct-3 proteins. These data suggest a functional interplay of winged helix/forkhead and POU-domain transcription factors on a variety of neuronal gene promoters.</description><subject>Animals</subject><subject>Aromatic-L-Amino-Acid Decarboxylases - genetics</subject><subject>Binding Sites - genetics</subject><subject>Biological and medical sciences</subject><subject>COS Cells</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-Binding Proteins - physiology</subject><subject>DOPA decarboxylase</subject><subject>Dopamine</subject><subject>Drifter</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene regulation</subject><subject>Helix-Loop-Helix Motifs</subject><subject>Hepatocyte Nuclear Factor 3-alpha</subject><subject>Hepatocyte Nuclear Factor 3-beta</subject><subject>Hepatocyte Nuclear Factor 3-gamma</subject><subject>Homeodomain Proteins</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>NF-Y</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nuclear Proteins - physiology</subject><subject>POU Domain Factors</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Sp1</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Factors - physiology</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Tumor Cells, Cultured</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEKtvCI1TyASE4mNpx_jgnBBUFpIpFggpu1sSedI0SO9hO1X0qXhFvd7XXXmYkz-8bf_ZXFOecveOMNxc_cumoKOXvN518yxirJK2fFCsu25I2XcWfFqsj8rw4jfFPhrjk_KQ46RpWclGvin-_rLtFQzY42vtcZ0hebxMSt-gRIZABdPKBCALOkO_rG2r8BNaROfiEuX8MjpYX3-haJypIbzPl7zCMMM95M4k2YSTekbTJO3EJ3sG4E09ZHogfyGaZwBHIJ5CsJiOFyTpPQFtDDGoIvb_fjhCR3KLDF8WzAcaILw_9rLi5-vTz8gu9Xn_-evnhmmpRl4lyyO-EQUhpaiGAyZqZpmdas740RnPoZINCdqJH2bCqFNK0g6kY1j1yia04K17v92arfxeMSU02ahxHcOiXqNpOdlLKx0HeVE1b8yqD9R7UwccYcFBzsBOEreJM7RJVD4mqXVyqk-ohUVVn3fnhgqWf0BxVhwjz_NVhDlHDOARw2sYjVoquadqdz_d7DPOv3VkMKmqLTqOxAXVSxttHjPwHI3u_gQ</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>Raynal, Jean-François</creator><creator>Dugast, Claire</creator><creator>Le Van Thaı̈, Agathe</creator><creator>Weber, Michel J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980501</creationdate><title>Winged helix hepatocyte nuclear factor 3 and POU-domain protein Brn-2/N-Oct-3 bind overlapping sites on the neuronal promoter of human aromatic l-amino acid decarboxylase gene</title><author>Raynal, Jean-François ; Dugast, Claire ; Le Van Thaı̈, Agathe ; Weber, Michel J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-1a811af388d533a0850d6b0cc0b2ddc1a986e3893be8604238d7fd40e5be18e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Aromatic-L-Amino-Acid Decarboxylases - genetics</topic><topic>Binding Sites - genetics</topic><topic>Biological and medical sciences</topic><topic>COS Cells</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DNA-Binding Proteins - physiology</topic><topic>DOPA decarboxylase</topic><topic>Dopamine</topic><topic>Drifter</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene regulation</topic><topic>Helix-Loop-Helix Motifs</topic><topic>Hepatocyte Nuclear Factor 3-alpha</topic><topic>Hepatocyte Nuclear Factor 3-beta</topic><topic>Hepatocyte Nuclear Factor 3-gamma</topic><topic>Homeodomain Proteins</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>NF-Y</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nuclear Proteins - physiology</topic><topic>POU Domain Factors</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Sp1</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Factors - physiology</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raynal, Jean-François</creatorcontrib><creatorcontrib>Dugast, Claire</creatorcontrib><creatorcontrib>Le Van Thaı̈, Agathe</creatorcontrib><creatorcontrib>Weber, Michel J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raynal, Jean-François</au><au>Dugast, Claire</au><au>Le Van Thaı̈, Agathe</au><au>Weber, Michel J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Winged helix hepatocyte nuclear factor 3 and POU-domain protein Brn-2/N-Oct-3 bind overlapping sites on the neuronal promoter of human aromatic l-amino acid decarboxylase gene</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>56</volume><issue>1</issue><spage>227</spage><epage>237</epage><pages>227-237</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>The neuronal promoter of human aromatic
l-amino acid decarboxylase gene has been analysed to elucidate the mechanisms of neuron type-specific expression. The (−560/+92) promoter segment was sufficient to direct luciferase expression at a higher level in SK-N-BE neuroblastoma cells, than in CHP126 neuroepithelia, HepG2 hepatoma or SK-Hep1 epithelioma cells. Deletions experiments showed that this segment contained a neuronal-specific (element T1) and a SK-N-BE-specific (element N1)
cis-activating sequences. Element T1 (−72/−36) bound Sp1 and NF-Y proteins, and unidentified neuronal-specific factors. Element N1 (−102/−72) bound cell-specific factors, identified as HNF-3, N-Oct-3/Brn-2 and N-Oct-2. HNF-3 proteins recognized the sequence TCAGTAAATA that matches the consensus motif. Oct-1, N-Oct-2 and N-Oct-3 bound the AAATAATGC sequence that overlaps the HNF-3 binding site. In addition, we show that the HNF-3 binding sites from aldolase C and
HNF-3β gene promoters also bind N-Oct-2 and N-Oct-3 proteins. These data suggest a functional interplay of winged helix/forkhead and POU-domain transcription factors on a variety of neuronal gene promoters.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9602135</pmid><doi>10.1016/S0169-328X(98)00048-5</doi><tpages>11</tpages></addata></record> |
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| ispartof | Brain research. Molecular brain research., 1998-05, Vol.56 (1), p.227-237 |
| issn | 0169-328X 1872-6941 |
| language | eng |
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| subjects | Animals Aromatic-L-Amino-Acid Decarboxylases - genetics Binding Sites - genetics Biological and medical sciences COS Cells DNA-Binding Proteins - metabolism DNA-Binding Proteins - physiology DOPA decarboxylase Dopamine Drifter Fundamental and applied biological sciences. Psychology Gene regulation Helix-Loop-Helix Motifs Hepatocyte Nuclear Factor 3-alpha Hepatocyte Nuclear Factor 3-beta Hepatocyte Nuclear Factor 3-gamma Homeodomain Proteins Humans Molecular and cellular biology Molecular genetics Neurons - cytology Neurons - metabolism NF-Y Nuclear Proteins - metabolism Nuclear Proteins - physiology POU Domain Factors Promoter Regions, Genetic - genetics Sp1 Transcription Factors - metabolism Transcription Factors - physiology Transcription. Transcription factor. Splicing. Rna processing Tumor Cells, Cultured |
| title | Winged helix hepatocyte nuclear factor 3 and POU-domain protein Brn-2/N-Oct-3 bind overlapping sites on the neuronal promoter of human aromatic l-amino acid decarboxylase gene |
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