Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain
Department of Virology, Central Veterinary Institute (CDI), Houtribweg 39, 8221 RA Lelystad, The Netherlands In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strong...
Gespeichert in:
| Veröffentlicht in: | Journal of general virology 1990-07, Vol.71 (7), p.1591-1595 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1595 |
|---|---|
| container_issue | 7 |
| container_start_page | 1591 |
| container_title | Journal of general virology |
| container_volume | 71 |
| creator | Moormann, Rob J. M de Rover, Tony Briaire, Jan Peeters, Ben P. H Gielkens, Arno L. J van Oirschot, Jan T |
| description | Department of Virology, Central Veterinary Institute (CDI), Houtribweg 39, 8221 RA Lelystad, The Netherlands
In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strongly reduced virulence in 10-week-old seronegative pigs, it could still cause severe disease or death in 3-day-old piglets. To attenuate the strain further, we constructed mutants with a deletion in the viral thymidine kinase gene. One mutant strain, designated 783, has a deletion of 19 base pairs and was shown to be highly immunogenic and safe for vaccination of pigs against pseudorabies virus.
Received 12 December 1989;
accepted 22 March 1990. |
| doi_str_mv | 10.1099/0022-1317-71-7-1591 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79895747</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79895747</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-4ca1335329550c1811412894b034b26941fd6b19dfaaea44069c2e1b2c1822383</originalsourceid><addsrcrecordid>eNqFkc2KFDEUhYMoY8_oE4iQlbgpzc1PpbKUYdSGATe6DrdSqe5o_bRJqod-Dx_Y1HQzLl2E5OZ85164h5A3wD4AM-YjY5xXIEBXGipdgTLwjGxA1qriRX9ONk_ES3Kd0k_GQEqlr8gVh1qBaDbkz3ZCl8MRc5gnOvc07305pzF0YfL0V5gwebrz5V1EpLst7fzgH-lxyTjl9f-Q_NLNEdvgEz2GuKRHS8RcaqQJe0_bJdOUwzDQfdjthxMN47hMc-GCo0d0bp2XcsQwvSIvehySf325b8iPz3ffb79W99--bG8_3VdOapUr6RCEUIIbpZiDBkACb4xsmZAtr42EvqtbMF2P6FFKVhvHPbS8sJyLRtyQd-e-hzj_XnzKdgzJ-WHAyc9Lsto0Rmmp_wuC0o009QqKM-jinFL0vT3EMGI8WWB2Dc2ukdg1EqvBaruGVlxvL-2XdvTdk-eSUtHfn_V1cw8helu2NoYyow2zLfv-1-ov_Dyhuw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15784967</pqid></control><display><type>article</type><title>Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Moormann, Rob J. M ; de Rover, Tony ; Briaire, Jan ; Peeters, Ben P. H ; Gielkens, Arno L. J ; van Oirschot, Jan T</creator><creatorcontrib>Moormann, Rob J. M ; de Rover, Tony ; Briaire, Jan ; Peeters, Ben P. H ; Gielkens, Arno L. J ; van Oirschot, Jan T</creatorcontrib><description>Department of Virology, Central Veterinary Institute (CDI), Houtribweg 39, 8221 RA Lelystad, The Netherlands
In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strongly reduced virulence in 10-week-old seronegative pigs, it could still cause severe disease or death in 3-day-old piglets. To attenuate the strain further, we constructed mutants with a deletion in the viral thymidine kinase gene. One mutant strain, designated 783, has a deletion of 19 base pairs and was shown to be highly immunogenic and safe for vaccination of pigs against pseudorabies virus.
Received 12 December 1989;
accepted 22 March 1990.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-71-7-1591</identifier><identifier>PMID: 2165138</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Animals ; Base Sequence ; Cell Line ; Chromosome Deletion ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; Genes, Viral ; Herpesvirus 1, Suid - enzymology ; Herpesvirus 1, Suid - genetics ; Herpesvirus 1, Suid - immunology ; Molecular Sequence Data ; Mutation ; Oligonucleotide Probes ; Restriction Mapping ; Swine ; Thymidine Kinase - genetics ; Viral Structural Proteins - genetics ; Viral Vaccines</subject><ispartof>Journal of general virology, 1990-07, Vol.71 (7), p.1591-1595</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-4ca1335329550c1811412894b034b26941fd6b19dfaaea44069c2e1b2c1822383</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2165138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moormann, Rob J. M</creatorcontrib><creatorcontrib>de Rover, Tony</creatorcontrib><creatorcontrib>Briaire, Jan</creatorcontrib><creatorcontrib>Peeters, Ben P. H</creatorcontrib><creatorcontrib>Gielkens, Arno L. J</creatorcontrib><creatorcontrib>van Oirschot, Jan T</creatorcontrib><title>Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Department of Virology, Central Veterinary Institute (CDI), Houtribweg 39, 8221 RA Lelystad, The Netherlands
In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strongly reduced virulence in 10-week-old seronegative pigs, it could still cause severe disease or death in 3-day-old piglets. To attenuate the strain further, we constructed mutants with a deletion in the viral thymidine kinase gene. One mutant strain, designated 783, has a deletion of 19 base pairs and was shown to be highly immunogenic and safe for vaccination of pigs against pseudorabies virus.
Received 12 December 1989;
accepted 22 March 1990.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Chromosome Deletion</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>Genes, Viral</subject><subject>Herpesvirus 1, Suid - enzymology</subject><subject>Herpesvirus 1, Suid - genetics</subject><subject>Herpesvirus 1, Suid - immunology</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Oligonucleotide Probes</subject><subject>Restriction Mapping</subject><subject>Swine</subject><subject>Thymidine Kinase - genetics</subject><subject>Viral Structural Proteins - genetics</subject><subject>Viral Vaccines</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2KFDEUhYMoY8_oE4iQlbgpzc1PpbKUYdSGATe6DrdSqe5o_bRJqod-Dx_Y1HQzLl2E5OZ85164h5A3wD4AM-YjY5xXIEBXGipdgTLwjGxA1qriRX9ONk_ES3Kd0k_GQEqlr8gVh1qBaDbkz3ZCl8MRc5gnOvc07305pzF0YfL0V5gwebrz5V1EpLst7fzgH-lxyTjl9f-Q_NLNEdvgEz2GuKRHS8RcaqQJe0_bJdOUwzDQfdjthxMN47hMc-GCo0d0bp2XcsQwvSIvehySf325b8iPz3ffb79W99--bG8_3VdOapUr6RCEUIIbpZiDBkACb4xsmZAtr42EvqtbMF2P6FFKVhvHPbS8sJyLRtyQd-e-hzj_XnzKdgzJ-WHAyc9Lsto0Rmmp_wuC0o009QqKM-jinFL0vT3EMGI8WWB2Dc2ukdg1EqvBaruGVlxvL-2XdvTdk-eSUtHfn_V1cw8helu2NoYyow2zLfv-1-ov_Dyhuw</recordid><startdate>19900701</startdate><enddate>19900701</enddate><creator>Moormann, Rob J. M</creator><creator>de Rover, Tony</creator><creator>Briaire, Jan</creator><creator>Peeters, Ben P. H</creator><creator>Gielkens, Arno L. J</creator><creator>van Oirschot, Jan T</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19900701</creationdate><title>Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain</title><author>Moormann, Rob J. M ; de Rover, Tony ; Briaire, Jan ; Peeters, Ben P. H ; Gielkens, Arno L. J ; van Oirschot, Jan T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-4ca1335329550c1811412894b034b26941fd6b19dfaaea44069c2e1b2c1822383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Chromosome Deletion</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>Genes, Viral</topic><topic>Herpesvirus 1, Suid - enzymology</topic><topic>Herpesvirus 1, Suid - genetics</topic><topic>Herpesvirus 1, Suid - immunology</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Oligonucleotide Probes</topic><topic>Restriction Mapping</topic><topic>Swine</topic><topic>Thymidine Kinase - genetics</topic><topic>Viral Structural Proteins - genetics</topic><topic>Viral Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moormann, Rob J. M</creatorcontrib><creatorcontrib>de Rover, Tony</creatorcontrib><creatorcontrib>Briaire, Jan</creatorcontrib><creatorcontrib>Peeters, Ben P. H</creatorcontrib><creatorcontrib>Gielkens, Arno L. J</creatorcontrib><creatorcontrib>van Oirschot, Jan T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moormann, Rob J. M</au><au>de Rover, Tony</au><au>Briaire, Jan</au><au>Peeters, Ben P. H</au><au>Gielkens, Arno L. J</au><au>van Oirschot, Jan T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1990-07-01</date><risdate>1990</risdate><volume>71</volume><issue>7</issue><spage>1591</spage><epage>1595</epage><pages>1591-1595</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Department of Virology, Central Veterinary Institute (CDI), Houtribweg 39, 8221 RA Lelystad, The Netherlands
In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strongly reduced virulence in 10-week-old seronegative pigs, it could still cause severe disease or death in 3-day-old piglets. To attenuate the strain further, we constructed mutants with a deletion in the viral thymidine kinase gene. One mutant strain, designated 783, has a deletion of 19 base pairs and was shown to be highly immunogenic and safe for vaccination of pigs against pseudorabies virus.
Received 12 December 1989;
accepted 22 March 1990.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>2165138</pmid><doi>10.1099/0022-1317-71-7-1591</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1990-07, Vol.71 (7), p.1591-1595 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79895747 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Cell Line Chromosome Deletion DNA, Viral - genetics DNA, Viral - isolation & purification Genes, Viral Herpesvirus 1, Suid - enzymology Herpesvirus 1, Suid - genetics Herpesvirus 1, Suid - immunology Molecular Sequence Data Mutation Oligonucleotide Probes Restriction Mapping Swine Thymidine Kinase - genetics Viral Structural Proteins - genetics Viral Vaccines |
| title | Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T17%3A54%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inactivation%20of%20the%20thymidine%20kinase%20gene%20of%20a%20gI%20deletion%20mutant%20of%20pseudorabies%20virus%20generates%20a%20safe%20but%20still%20highly%20immunogenic%20vaccine%20strain&rft.jtitle=Journal%20of%20general%20virology&rft.au=Moormann,%20Rob%20J.%20M&rft.date=1990-07-01&rft.volume=71&rft.issue=7&rft.spage=1591&rft.epage=1595&rft.pages=1591-1595&rft.issn=0022-1317&rft.eissn=1465-2099&rft_id=info:doi/10.1099/0022-1317-71-7-1591&rft_dat=%3Cproquest_cross%3E79895747%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15784967&rft_id=info:pmid/2165138&rfr_iscdi=true |