Entamoeba histolytica: Correlation of the cytopathic effect of virulent trophozoites with secretion of a cysteine proteinase
Work from several laboratories suggests a correlation between expression of cysteine proteinase activity and the cytopathic effect of virulent HM1 strain Entamoeba histolytica trophozoites on cultured cell monolayers. Consistent with this relationship, we find that L-6 trophozoites, mutants cloned f...
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| Veröffentlicht in: | Experimental parasitology 1990-08, Vol.71 (2), p.199-206 |
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| creator | Keene, William E. Hidalgo, Maria Eugenia Orozco, Esther McKerrow, James H. |
| description | Work from several laboratories suggests a correlation between expression of cysteine proteinase activity and the cytopathic effect of virulent HM1 strain
Entamoeba histolytica trophozoites on cultured cell monolayers. Consistent with this relationship, we find that L-6 trophozoites, mutants cloned from the HM1 parent strain, are deficient in both proteinase expression and cytopathic effect. Three other clones, with proteinase expression equal to or greater than that of the HM1 strain, express the cytopathic effect. Furthermore, a nontoxic specific proteinase inhibitor, Z-phenylalanyl-alanyl-CH
2F, inhibits the cytopathic effect of live trophozoites in a dose-dependent manner. These results support the hypothesis that expression and release of the cysteine proteinase is an important factor in producing the cytopathic effect, presumably by its degradation of cell anchoring proteins. |
| doi_str_mv | 10.1016/0014-4894(90)90022-5 |
| format | Article |
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Entamoeba histolytica trophozoites on cultured cell monolayers. Consistent with this relationship, we find that L-6 trophozoites, mutants cloned from the HM1 parent strain, are deficient in both proteinase expression and cytopathic effect. Three other clones, with proteinase expression equal to or greater than that of the HM1 strain, express the cytopathic effect. Furthermore, a nontoxic specific proteinase inhibitor, Z-phenylalanyl-alanyl-CH
2F, inhibits the cytopathic effect of live trophozoites in a dose-dependent manner. These results support the hypothesis that expression and release of the cysteine proteinase is an important factor in producing the cytopathic effect, presumably by its degradation of cell anchoring proteins.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cysteine Endopeptidases - biosynthesis</subject><subject>Cysteine Endopeptidases - metabolism</subject><subject>Cysteine Proteinase Inhibitors</subject><subject>Entamoeba histolytica</subject><subject>Entamoeba histolytica - enzymology</subject><subject>Entamoeba histolytica - genetics</subject><subject>Entamoeba histolytica - pathogenicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Invertebrates</subject><subject>Life cycle. Host-agent relationship. 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Pathogenesis</topic><topic>Mutation</topic><topic>Proteinase</topic><topic>Protozoa</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keene, William E.</creatorcontrib><creatorcontrib>Hidalgo, Maria Eugenia</creatorcontrib><creatorcontrib>Orozco, Esther</creatorcontrib><creatorcontrib>McKerrow, James H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keene, William E.</au><au>Hidalgo, Maria Eugenia</au><au>Orozco, Esther</au><au>McKerrow, James H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Entamoeba histolytica: Correlation of the cytopathic effect of virulent trophozoites with secretion of a cysteine proteinase</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>1990-08-01</date><risdate>1990</risdate><volume>71</volume><issue>2</issue><spage>199</spage><epage>206</epage><pages>199-206</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>Work from several laboratories suggests a correlation between expression of cysteine proteinase activity and the cytopathic effect of virulent HM1 strain
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| ispartof | Experimental parasitology, 1990-08, Vol.71 (2), p.199-206 |
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| subjects | Animals Biological and medical sciences Cell Line Cysteine Endopeptidases - biosynthesis Cysteine Endopeptidases - metabolism Cysteine Proteinase Inhibitors Entamoeba histolytica Entamoeba histolytica - enzymology Entamoeba histolytica - genetics Entamoeba histolytica - pathogenicity Fundamental and applied biological sciences. Psychology Invertebrates Life cycle. Host-agent relationship. Pathogenesis Mutation Proteinase Protozoa Virulence |
| title | Entamoeba histolytica: Correlation of the cytopathic effect of virulent trophozoites with secretion of a cysteine proteinase |
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