Expression of a yeast glycolytic gene is subject to dosage limitation

The Saccharomyces cerevisiae kinase-encoding gene ( PYK1) has been transformed back into yeast using a derivative of the multicopy vector, pJDB207. High levels of PYK1 expression in these transformants are limited by at least two separate mechanisms. Pyruvate kinase assays and polysome analyses demo...

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Veröffentlicht in:	Gene 1990-04, Vol.89 (1), p.85-92
Hauptverfasser:	Moore, Paul A., Bettany, Andrew J.E., Brown, Alistair J.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation, Fungal gene regulation Genetic Vectors Glycolysis Kinetics messenger RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data Oligonucleotide Probes Plasmids polysomes pyruvate kinase Pyruvate Kinase - genetics Pyruvate Kinase - metabolism Recombinant DNA RNA, Messenger - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics transcription translation
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