Measurement of Human Immunodeficiency Virus Type 1 Plasma Virus Load Based on Reverse Transcriptase (RT) Activity: Evidence of Variabilities in Levels of Virion-Associated RT
Virus load based on levels of functional reverse transcriptase (RT) was measured in plasma from 50 human immunodeficiency virus (HIV) type 1-infected persons, in 87 samples from 10 HIV-1 seroconversion panels, and in 100 uninfected persons by use of Amp-RT, an ultrasensitive RT assay. Of the 50 clin...
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| Veröffentlicht in: | The Journal of infectious diseases 1998-05, Vol.177 (5), p.1221-1229 |
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| container_title | The Journal of infectious diseases |
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| creator | Lerma, J. Gerardo García Yamamoto, Shinji Gómez-Cano, María Soriano, Vincent Green, Timothy A. Busch, Michael P. Folks, Thomas M. Heneine, Walid |
| description | Virus load based on levels of functional reverse transcriptase (RT) was measured in plasma from 50 human immunodeficiency virus (HIV) type 1-infected persons, in 87 samples from 10 HIV-1 seroconversion panels, and in 100 uninfected persons by use of Amp-RT, an ultrasensitive RT assay. Of the 50 clinical samples, 38 (76%) were Amp-RT positive, while all uninfected controls were negative. Pearson's correlation coefficient of RNA and RT levels was .73 for all samples, .86 for seroconversion samples, and .49 for clinical samples. Calculated ratios of RT activity to virion RNA varied widely during both early and late stages of infection. Mean RT:RNA ratios in 8 seroconversion panels and in 12 (34.3%) of 35 individual clinical samples were significantly lower than the ratio for a reference virus. However, ratios were stable in individual seroconversions over time. These data demonstrate that RT activity can be used to quantitate plasma virus load and provide evidence of different levels of virion-associated RT among HIV-1-infected persons. |
| doi_str_mv | 10.1086/515272 |
| format | Article |
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Gerardo García ; Yamamoto, Shinji ; Gómez-Cano, María ; Soriano, Vincent ; Green, Timothy A. ; Busch, Michael P. ; Folks, Thomas M. ; Heneine, Walid</creator><creatorcontrib>Lerma, J. Gerardo García ; Yamamoto, Shinji ; Gómez-Cano, María ; Soriano, Vincent ; Green, Timothy A. ; Busch, Michael P. ; Folks, Thomas M. ; Heneine, Walid</creatorcontrib><description>Virus load based on levels of functional reverse transcriptase (RT) was measured in plasma from 50 human immunodeficiency virus (HIV) type 1-infected persons, in 87 samples from 10 HIV-1 seroconversion panels, and in 100 uninfected persons by use of Amp-RT, an ultrasensitive RT assay. Of the 50 clinical samples, 38 (76%) were Amp-RT positive, while all uninfected controls were negative. Pearson's correlation coefficient of RNA and RT levels was .73 for all samples, .86 for seroconversion samples, and .49 for clinical samples. Calculated ratios of RT activity to virion RNA varied widely during both early and late stages of infection. Mean RT:RNA ratios in 8 seroconversion panels and in 12 (34.3%) of 35 individual clinical samples were significantly lower than the ratio for a reference virus. However, ratios were stable in individual seroconversions over time. These data demonstrate that RT activity can be used to quantitate plasma virus load and provide evidence of different levels of virion-associated RT among HIV-1-infected persons.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/515272</identifier><identifier>PMID: 9593007</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Acquired Immunodeficiency Syndrome - blood ; Acquired Immunodeficiency Syndrome - enzymology ; AIDS/HIV ; Biological and medical sciences ; Blood plasma ; DNA Primers ; HIV ; HIV 1 ; HIV Reverse Transcriptase - blood ; HIV Seronegativity ; HIV Seropositivity - blood ; HIV Seropositivity - enzymology ; HIV-1 - isolation & purification ; Human viral diseases ; Humans ; Infections ; Infectious diseases ; Major Articles ; Medical sciences ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Ratios ; Regression Analysis ; RNA ; RNA, Viral - blood ; Self sustained sequence replication ; Sensitivity and Specificity ; Time Factors ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. 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Gerardo García</creatorcontrib><creatorcontrib>Yamamoto, Shinji</creatorcontrib><creatorcontrib>Gómez-Cano, María</creatorcontrib><creatorcontrib>Soriano, Vincent</creatorcontrib><creatorcontrib>Green, Timothy A.</creatorcontrib><creatorcontrib>Busch, Michael P.</creatorcontrib><creatorcontrib>Folks, Thomas M.</creatorcontrib><creatorcontrib>Heneine, Walid</creatorcontrib><title>Measurement of Human Immunodeficiency Virus Type 1 Plasma Virus Load Based on Reverse Transcriptase (RT) Activity: Evidence of Variabilities in Levels of Virion-Associated RT</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>Virus load based on levels of functional reverse transcriptase (RT) was measured in plasma from 50 human immunodeficiency virus (HIV) type 1-infected persons, in 87 samples from 10 HIV-1 seroconversion panels, and in 100 uninfected persons by use of Amp-RT, an ultrasensitive RT assay. Of the 50 clinical samples, 38 (76%) were Amp-RT positive, while all uninfected controls were negative. Pearson's correlation coefficient of RNA and RT levels was .73 for all samples, .86 for seroconversion samples, and .49 for clinical samples. Calculated ratios of RT activity to virion RNA varied widely during both early and late stages of infection. Mean RT:RNA ratios in 8 seroconversion panels and in 12 (34.3%) of 35 individual clinical samples were significantly lower than the ratio for a reference virus. However, ratios were stable in individual seroconversions over time. These data demonstrate that RT activity can be used to quantitate plasma virus load and provide evidence of different levels of virion-associated RT among HIV-1-infected persons.</description><subject>Acquired Immunodeficiency Syndrome - blood</subject><subject>Acquired Immunodeficiency Syndrome - enzymology</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Blood plasma</subject><subject>DNA Primers</subject><subject>HIV</subject><subject>HIV 1</subject><subject>HIV Reverse Transcriptase - blood</subject><subject>HIV Seronegativity</subject><subject>HIV Seropositivity - blood</subject><subject>HIV Seropositivity - enzymology</subject><subject>HIV-1 - isolation & purification</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Major Articles</subject><subject>Medical sciences</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Ratios</subject><subject>Regression Analysis</subject><subject>RNA</subject><subject>RNA, Viral - blood</subject><subject>Self sustained sequence replication</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Viral Load</subject><subject>Virion - enzymology</subject><subject>Virion - isolation & purification</subject><subject>Virions</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u00AUhS0EKmmBN0CaBUJlYZhfj80uVC2pFKBEplRsrOvxtTTFP2GuHZGX4hlxSJQuWY10v0_nSHOi6IXgbwVPk3dGGGnlo2gmjLJxkgj1OJpxLmUs0ix7Gp0S3XPOtUrsSXSSmUxxbmfRn08INAZssRtYX7PF2ELHrtt27PoKa-88dm7Lbn0YieXbNTLBbhqgFg63ZQ8V-wCEFes7tsINBkKWB-jIBb8eJsLOV_kbNneD3_hh-55dbnw1peKu7xaCh9I3fvBIzHdsOSU09A_54PsunhP1zsMwFazyZ9GTGhrC54f3LPp2dZlfLOLll4_XF_Nl7LRMhhhQyQxMCoKXyDXUpXHGaJOVUmXSGcur0mpXiRS1rFPgoLR2GnUt0Gao1Fn0ep-7Dv2vEWkoWk8OmwY67EcqbJamNpXiv6JItEkklw-iCz1RwLpYB99C2BaCF7sFi_2Ck_jykDiWLVZH7TDZxF8dOJCDpp5-2nk6alJqa1P1EHNPQx-OWPGpS-hdTbznngb8feQQfhaJVdYUi7sfxVcuP-ff766KG_UXMVu76w</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>Lerma, J. Gerardo García</creator><creator>Yamamoto, Shinji</creator><creator>Gómez-Cano, María</creator><creator>Soriano, Vincent</creator><creator>Green, Timothy A.</creator><creator>Busch, Michael P.</creator><creator>Folks, Thomas M.</creator><creator>Heneine, Walid</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980501</creationdate><title>Measurement of Human Immunodeficiency Virus Type 1 Plasma Virus Load Based on Reverse Transcriptase (RT) Activity: Evidence of Variabilities in Levels of Virion-Associated RT</title><author>Lerma, J. 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Gerardo García</au><au>Yamamoto, Shinji</au><au>Gómez-Cano, María</au><au>Soriano, Vincent</au><au>Green, Timothy A.</au><au>Busch, Michael P.</au><au>Folks, Thomas M.</au><au>Heneine, Walid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of Human Immunodeficiency Virus Type 1 Plasma Virus Load Based on Reverse Transcriptase (RT) Activity: Evidence of Variabilities in Levels of Virion-Associated RT</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>177</volume><issue>5</issue><spage>1221</spage><epage>1229</epage><pages>1221-1229</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Virus load based on levels of functional reverse transcriptase (RT) was measured in plasma from 50 human immunodeficiency virus (HIV) type 1-infected persons, in 87 samples from 10 HIV-1 seroconversion panels, and in 100 uninfected persons by use of Amp-RT, an ultrasensitive RT assay. 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| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1998-05, Vol.177 (5), p.1221-1229 |
| issn | 0022-1899 1537-6613 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Acquired Immunodeficiency Syndrome - blood Acquired Immunodeficiency Syndrome - enzymology AIDS/HIV Biological and medical sciences Blood plasma DNA Primers HIV HIV 1 HIV Reverse Transcriptase - blood HIV Seronegativity HIV Seropositivity - blood HIV Seropositivity - enzymology HIV-1 - isolation & purification Human viral diseases Humans Infections Infectious diseases Major Articles Medical sciences Polymerase chain reaction Polymerase Chain Reaction - methods Ratios Regression Analysis RNA RNA, Viral - blood Self sustained sequence replication Sensitivity and Specificity Time Factors Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral Load Virion - enzymology Virion - isolation & purification Virions Viruses |
| title | Measurement of Human Immunodeficiency Virus Type 1 Plasma Virus Load Based on Reverse Transcriptase (RT) Activity: Evidence of Variabilities in Levels of Virion-Associated RT |
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