A Function for the AP3 Coat Complex in Synaptic Vesicle Formation from Endosomes
Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgi-to-vacuole traffic in yeast. Depletion of...
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| Veröffentlicht in: | Cell 1998-05, Vol.93 (3), p.423-432 |
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| creator | Faúndez, Victor Horng, Jim-Tong Kelly, Regis B |
| description | Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgi-to-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes. |
| doi_str_mv | 10.1016/S0092-8674(00)81170-8 |
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The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgi-to-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. 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We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.</description><subject>Adaptor Protein Complex 3</subject><subject>Adaptor Protein Complex alpha Subunits</subject><subject>Adaptor Protein Complex beta Subunits</subject><subject>Adaptor Proteins, Vesicular Transport</subject><subject>Adenosine Triphosphate - physiology</subject><subject>ADP-Ribosylation Factor 1</subject><subject>ADP-Ribosylation Factors</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Brain</subject><subject>Cytosol - metabolism</subject><subject>Endosomes - physiology</subject><subject>Female</subject><subject>GTP-Binding Proteins - analysis</subject><subject>GTP-Binding Proteins - physiology</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate)</subject><subject>Guanosine Triphosphate</subject><subject>Membrane Proteins - analysis</subject><subject>Membrane Proteins - physiology</subject><subject>Monomeric Clathrin Assembly Proteins</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Nerve Tissue Proteins - physiology</subject><subject>PC12 Cells</subject><subject>Phosphoproteins - analysis</subject><subject>Phosphoproteins - physiology</subject><subject>Pronase - antagonists & inhibitors</subject><subject>Pronase - pharmacology</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Synaptic Vesicles - chemistry</subject><subject>Synaptic Vesicles - physiology</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMoun78BCEn0UN1kjRNcpJlcVUQFPy4hm46wUjbrElX9N9b3cWrl5nD-7wz8BByzOCcAasuHgEML3SlylOAM82YgkJvkQkDo4qSKb5NJn_IHtnP-Q0AtJRyl-waaYCpakIepnS-6t0QYk99THR4RTp9EHQW62Ec3bLFTxp6-vjV18shOPqCObgW6Tymrl7XUuzoVd_EHDvMh2TH123Go80-IM_zq6fZTXF3f307m94VTspqKJzQjTcNQ186B9qrplaeoxJ8UWtnKu9QM9EoKYyXC8G4R-MQleQLpiUvxQE5Wd9dpvi-wjzYLmSHbVv3GFfZKqM155L_C7KqlCUIMYJyDboUc07o7TKFrk5floH9UW5_ldsfnxbA_iq3euwdbx6sFh02f62N4zG_XOc46vgImGx2AXuHTUjoBtvE8M-Hb4mUj_w</recordid><startdate>19980501</startdate><enddate>19980501</enddate><creator>Faúndez, Victor</creator><creator>Horng, Jim-Tong</creator><creator>Kelly, Regis B</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19980501</creationdate><title>A Function for the AP3 Coat Complex in Synaptic Vesicle Formation from Endosomes</title><author>Faúndez, Victor ; Horng, Jim-Tong ; Kelly, Regis B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-c38df9d1ef4cc08f7da7f2e732ba8c96fce813d7539f5b312fe9cee752b185243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adaptor Protein Complex 3</topic><topic>Adaptor Protein Complex alpha Subunits</topic><topic>Adaptor Protein Complex beta Subunits</topic><topic>Adaptor Proteins, Vesicular Transport</topic><topic>Adenosine Triphosphate - physiology</topic><topic>ADP-Ribosylation Factor 1</topic><topic>ADP-Ribosylation Factors</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>Brain</topic><topic>Cytosol - metabolism</topic><topic>Endosomes - physiology</topic><topic>Female</topic><topic>GTP-Binding Proteins - analysis</topic><topic>GTP-Binding Proteins - physiology</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate)</topic><topic>Guanosine Triphosphate</topic><topic>Membrane Proteins - analysis</topic><topic>Membrane Proteins - physiology</topic><topic>Monomeric Clathrin Assembly Proteins</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Nerve Tissue Proteins - physiology</topic><topic>PC12 Cells</topic><topic>Phosphoproteins - analysis</topic><topic>Phosphoproteins - physiology</topic><topic>Pronase - antagonists & inhibitors</topic><topic>Pronase - pharmacology</topic><topic>Protease Inhibitors - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Synaptic Vesicles - chemistry</topic><topic>Synaptic Vesicles - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Faúndez, Victor</creatorcontrib><creatorcontrib>Horng, Jim-Tong</creatorcontrib><creatorcontrib>Kelly, Regis B</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Faúndez, Victor</au><au>Horng, Jim-Tong</au><au>Kelly, Regis B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Function for the AP3 Coat Complex in Synaptic Vesicle Formation from Endosomes</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1998-05-01</date><risdate>1998</risdate><volume>93</volume><issue>3</issue><spage>423</spage><epage>432</epage><pages>423-432</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgi-to-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9590176</pmid><doi>10.1016/S0092-8674(00)81170-8</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Adaptor Protein Complex 3 Adaptor Protein Complex alpha Subunits Adaptor Protein Complex beta Subunits Adaptor Proteins, Vesicular Transport Adenosine Triphosphate - physiology ADP-Ribosylation Factor 1 ADP-Ribosylation Factors Animals Binding Sites Brain Cytosol - metabolism Endosomes - physiology Female GTP-Binding Proteins - analysis GTP-Binding Proteins - physiology Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate Membrane Proteins - analysis Membrane Proteins - physiology Monomeric Clathrin Assembly Proteins Nerve Tissue Proteins - analysis Nerve Tissue Proteins - physiology PC12 Cells Phosphoproteins - analysis Phosphoproteins - physiology Pronase - antagonists & inhibitors Pronase - pharmacology Protease Inhibitors - pharmacology Rats Rats, Sprague-Dawley Synaptic Vesicles - chemistry Synaptic Vesicles - physiology |
| title | A Function for the AP3 Coat Complex in Synaptic Vesicle Formation from Endosomes |
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