Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its overexpression in Escherichia coli

Phytase hydrolyzes phytate to release inorganic phosphate, which would decrease the addition of phosphorus to feedstuffs for monogastric animals and thus reduce environmental pollution. The gene encoding phytase from Bacillus sp. DS11 was cloned in Escherichia coli and its sequence determined. A 560...

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Veröffentlicht in:	FEMS microbiology letters 1998-05, Vol.162 (1), p.185-191
Hauptverfasser:	Kim, Y.O, Lee, J.K, Kim, H.K, Yu, J.H, Oh, T.K
Format:	Artikel
Sprache:	eng
Schlagworte:	6-Phytase - chemistry 6-Phytase - genetics Amino Acid Sequence Amino acids Bacillus Bacillus - enzymology Bacillus - genetics Bacteriology Base Sequence Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cloning Cloning, Molecular Cyanogen Deoxyribonucleic acid DNA E coli Enzyme Stability Escherichia coli Escherichia coli - genetics feed additives Fundamental and applied biological sciences. Psychology genbank/u85968 Gene Expression gene overexpression Gene sequencing Genes, Bacterial - genetics Genetics Hot Temperature inorganic phosphorus Microbiology Mission oriented research Molecular Sequence Data Molecular Weight monogastric animals monogastric livestock Nucleotide sequence Nucleotides Oligonucleotides Overexpression Phosphorus phy gene Phytase Phytate Pollution control Polypeptides poultry Proteins Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Restriction Mapping Sequence Analysis Sequence Analysis, DNA structural genes swine
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