Cytoplasmic staining of c‐erbB‐2 is not associated with the presence of detectable c‐erbB‐2 mRNA in breast cancer specimens
The cell‐surface receptor tyrosine kinase protein c‐erbB‐2 is immunocytochemically detected as membrane staining on the surface of cancer cells in 20–30% of cases of breast cancer, and its presence has been associated with poor prognosis for the patient. However, there have been numerous reports of...
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| Veröffentlicht in: | International journal of cancer 1998-05, Vol.76 (4), p.459-463 |
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| container_title | International journal of cancer |
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| creator | Taylor, Susan L. Platt‐Higgins, Angela Rudland, Philip S. Winstanley, John H. R. Barraclough, Roger |
| description | The cell‐surface receptor tyrosine kinase protein c‐erbB‐2 is immunocytochemically detected as membrane staining on the surface of cancer cells in 20–30% of cases of breast cancer, and its presence has been associated with poor prognosis for the patient. However, there have been numerous reports of immunocytochemical staining for c‐erbB‐2 solely in the cytoplasm of some normal and tumour specimens with frequently used anti‐sera, and the presence of such staining has been difficult to interpret. It is not known for certain that cytoplasmic c‐erbB‐2 staining is an artefact of the immunocytochemical procedures used. Thus, mRNA for c‐erbB‐2 has been quantified in tumours exhibiting only cytoplasmic staining or varying levels of membrane staining using a sensitive, competitive PCR method. Whereas abundant levels of c‐erbB‐2 mRNA are found in tumours exhibiting membrane staining for c‐erbB‐2 and these levels correlate with the percentage of tumour cells showing membranous staining for c‐erbB‐2, the level of c‐erbB‐2 mRNA in tumours displaying only cytoplasmic staining is no higher than in c‐erbB‐2‐negative specimens. Int. J. Cancer 76:459–463, 1998.© 1998 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1097-0215(19980518)76:4<459::AID-IJC2>3.0.CO;2-Q |
| format | Article |
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Whereas abundant levels of c‐erbB‐2 mRNA are found in tumours exhibiting membrane staining for c‐erbB‐2 and these levels correlate with the percentage of tumour cells showing membranous staining for c‐erbB‐2, the level of c‐erbB‐2 mRNA in tumours displaying only cytoplasmic staining is no higher than in c‐erbB‐2‐negative specimens. Int. J. Cancer 76:459–463, 1998.© 1998 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/(SICI)1097-0215(19980518)76:4<459::AID-IJC2>3.0.CO;2-Q</identifier><identifier>PMID: 9590117</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; Breast Neoplasms - metabolism ; Breast Neoplasms - ultrastructure ; Cytoplasm - metabolism ; Genital system. Mammary gland ; Humans ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Polymerase Chain Reaction ; Receptor, ErbB-2 - metabolism ; RNA, Messenger - analysis</subject><ispartof>International journal of cancer, 1998-05, Vol.76 (4), p.459-463</ispartof><rights>Copyright © 1998 Wiley‐Liss, Inc.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4242-3e82b3deb2e408fa94b8f29791702b3a5b346d726155208d03da081c3133e8773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-0215%2819980518%2976%3A4%3C459%3A%3AAID-IJC2%3E3.0.CO%3B2-Q$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-0215%2819980518%2976%3A4%3C459%3A%3AAID-IJC2%3E3.0.CO%3B2-Q$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2221062$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9590117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taylor, Susan L.</creatorcontrib><creatorcontrib>Platt‐Higgins, Angela</creatorcontrib><creatorcontrib>Rudland, Philip S.</creatorcontrib><creatorcontrib>Winstanley, John H. R.</creatorcontrib><creatorcontrib>Barraclough, Roger</creatorcontrib><title>Cytoplasmic staining of c‐erbB‐2 is not associated with the presence of detectable c‐erbB‐2 mRNA in breast cancer specimens</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>The cell‐surface receptor tyrosine kinase protein c‐erbB‐2 is immunocytochemically detected as membrane staining on the surface of cancer cells in 20–30% of cases of breast cancer, and its presence has been associated with poor prognosis for the patient. However, there have been numerous reports of immunocytochemical staining for c‐erbB‐2 solely in the cytoplasm of some normal and tumour specimens with frequently used anti‐sera, and the presence of such staining has been difficult to interpret. It is not known for certain that cytoplasmic c‐erbB‐2 staining is an artefact of the immunocytochemical procedures used. Thus, mRNA for c‐erbB‐2 has been quantified in tumours exhibiting only cytoplasmic staining or varying levels of membrane staining using a sensitive, competitive PCR method. Whereas abundant levels of c‐erbB‐2 mRNA are found in tumours exhibiting membrane staining for c‐erbB‐2 and these levels correlate with the percentage of tumour cells showing membranous staining for c‐erbB‐2, the level of c‐erbB‐2 mRNA in tumours displaying only cytoplasmic staining is no higher than in c‐erbB‐2‐negative specimens. Int. J. Cancer 76:459–463, 1998.© 1998 Wiley‐Liss, Inc.</description><subject>Biological and medical sciences</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - ultrastructure</subject><subject>Cytoplasm - metabolism</subject><subject>Genital system. Mammary gland</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Polymerase Chain Reaction</subject><subject>Receptor, ErbB-2 - metabolism</subject><subject>RNA, Messenger - analysis</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdtqFEEURRtR4hj9BKEeRJKHHuvWXV2jBMaOl5HgEG-vh-rq06akb3b1EOYt4A_4jX6J1WYcEYU8nSrO3pvNWVF0wuicUcqfHL1f5atjRrWKKWfJEdM6ownLjlW6kM9koheL5eo0Xr3J-YmY03m-fsrj81vRbG-5Hc1CEI0VE-nd6J73XyhlLKHyIDrQiQ5vNYu-5dux62vjG2eJH41rXfuZdBWxP66-41A8D4MT50nbjcR431lnRizJpRsvyHiBpB_QY2tx8pQ4oh1NUePf9ubd2yVxLSkGNH4k1gT9QHyP1jXY-vvRncrUHh_s5mH08eWLD_nr-Gz9apUvz2IrueSxwIwXosSCo6RZZbQssoprpZmiYWGSQsi0VDxlScJpVlJRGpoxK5gIVqXEYfT4Orcfuq8b9CM0zlusa9Nit_GgdKYypfmNQpbKcMhMBuGna6EdOu8HrKAfXGOGLTAKE0aACSNMTGBiAr8xgkpBQsAIEDDChBEEUMjXwOE8BD_cNdgUDZb72B23sH-02xtvTV0N4aTO72Wcc0ZT_qffpatx-0-5G7v9p9qvv_gJAbjHVA</recordid><startdate>19980518</startdate><enddate>19980518</enddate><creator>Taylor, Susan L.</creator><creator>Platt‐Higgins, Angela</creator><creator>Rudland, Philip S.</creator><creator>Winstanley, John H. R.</creator><creator>Barraclough, Roger</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980518</creationdate><title>Cytoplasmic staining of c‐erbB‐2 is not associated with the presence of detectable c‐erbB‐2 mRNA in breast cancer specimens</title><author>Taylor, Susan L. ; Platt‐Higgins, Angela ; Rudland, Philip S. ; Winstanley, John H. R. ; Barraclough, Roger</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4242-3e82b3deb2e408fa94b8f29791702b3a5b346d726155208d03da081c3133e8773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - ultrastructure</topic><topic>Cytoplasm - metabolism</topic><topic>Genital system. Mammary gland</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Polymerase Chain Reaction</topic><topic>Receptor, ErbB-2 - metabolism</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taylor, Susan L.</creatorcontrib><creatorcontrib>Platt‐Higgins, Angela</creatorcontrib><creatorcontrib>Rudland, Philip S.</creatorcontrib><creatorcontrib>Winstanley, John H. 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R.</au><au>Barraclough, Roger</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytoplasmic staining of c‐erbB‐2 is not associated with the presence of detectable c‐erbB‐2 mRNA in breast cancer specimens</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1998-05-18</date><risdate>1998</risdate><volume>76</volume><issue>4</issue><spage>459</spage><epage>463</epage><pages>459-463</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>The cell‐surface receptor tyrosine kinase protein c‐erbB‐2 is immunocytochemically detected as membrane staining on the surface of cancer cells in 20–30% of cases of breast cancer, and its presence has been associated with poor prognosis for the patient. However, there have been numerous reports of immunocytochemical staining for c‐erbB‐2 solely in the cytoplasm of some normal and tumour specimens with frequently used anti‐sera, and the presence of such staining has been difficult to interpret. It is not known for certain that cytoplasmic c‐erbB‐2 staining is an artefact of the immunocytochemical procedures used. Thus, mRNA for c‐erbB‐2 has been quantified in tumours exhibiting only cytoplasmic staining or varying levels of membrane staining using a sensitive, competitive PCR method. Whereas abundant levels of c‐erbB‐2 mRNA are found in tumours exhibiting membrane staining for c‐erbB‐2 and these levels correlate with the percentage of tumour cells showing membranous staining for c‐erbB‐2, the level of c‐erbB‐2 mRNA in tumours displaying only cytoplasmic staining is no higher than in c‐erbB‐2‐negative specimens. Int. J. 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| ispartof | International journal of cancer, 1998-05, Vol.76 (4), p.459-463 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Breast Neoplasms - metabolism Breast Neoplasms - ultrastructure Cytoplasm - metabolism Genital system. Mammary gland Humans Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Receptor, ErbB-2 - metabolism RNA, Messenger - analysis |
| title | Cytoplasmic staining of c‐erbB‐2 is not associated with the presence of detectable c‐erbB‐2 mRNA in breast cancer specimens |
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