Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch
Abstract Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element. This DNA switch directs transcription of fimA, encoding the major type 1 fimbrial subunit, in the on orientation but not in the off orientation. Inversion of the DNA element r...
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| Veröffentlicht in: | FEMS microbiology letters 1998-05, Vol.162 (1), p.17-23 |
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| creator | Olsen, Peter B Schembri, Mark A Gally, David L Klemm, Per |
| description | Abstract
Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element. This DNA switch directs transcription of fimA, encoding the major type 1 fimbrial subunit, in the on orientation but not in the off orientation. Inversion of the DNA element requires either FimB (confers both on and off orientations) or FimE (confers off orientation). Here we show, by chromosomally located fimB- and fimE-lacZ cassettes in isogenic strain sets differing only in the hns locus, how the global regulator H-NS affects the expression of type 1 fimbriae. H-NS was found to downregulate fimB and fimE in a temperature-dependent manner which affected the genes inversely at 30°C and 37°C. By gel-retardation assays H-NS binding was demonstrated to the regions containing the fimB promoter and the fimE promoter, respectively. In vitro recombination analysis suggested no direct involvement of H-NS in the inversion of the phase switch. Rather than directly affecting the switching process per se, it appeared that the orientation of this element was affected by the differential temperature modulation of H-NS of the fimB and fimE genes. Taken together the results suggest that H-NS modulates expression of type 1 fimbriae in a way which seems to favor a fimbriate state at the mammalian body temperature. |
| doi_str_mv | 10.1111/j.1574-6968.1998.tb12973.x |
| format | Article |
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Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element. This DNA switch directs transcription of fimA, encoding the major type 1 fimbrial subunit, in the on orientation but not in the off orientation. Inversion of the DNA element requires either FimB (confers both on and off orientations) or FimE (confers off orientation). Here we show, by chromosomally located fimB- and fimE-lacZ cassettes in isogenic strain sets differing only in the hns locus, how the global regulator H-NS affects the expression of type 1 fimbriae. H-NS was found to downregulate fimB and fimE in a temperature-dependent manner which affected the genes inversely at 30°C and 37°C. By gel-retardation assays H-NS binding was demonstrated to the regions containing the fimB promoter and the fimE promoter, respectively. In vitro recombination analysis suggested no direct involvement of H-NS in the inversion of the phase switch. Rather than directly affecting the switching process per se, it appeared that the orientation of this element was affected by the differential temperature modulation of H-NS of the fimB and fimE genes. Taken together the results suggest that H-NS modulates expression of type 1 fimbriae in a way which seems to favor a fimbriate state at the mammalian body temperature.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1998.tb12973.x</identifier><identifier>PMID: 9595659</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Body temperature ; Cassettes ; Deoxyribonucleic acid ; DNA ; DNA Nucleotidyltransferases - genetics ; DNA, Bacterial - metabolism ; DNA, Recombinant ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; E coli ; Escherichia coli - genetics ; Escherichia coli - physiology ; Escherichia coli Proteins ; Fimbriae, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Bacterial - physiology ; Genes ; Genes, Bacterial - genetics ; Genetics ; H‐NS ; H‐NS binding ; Integrases ; Inversion ; Microbiology ; Modulation ; Orientation ; Phase variations ; Pili ; Promoter Regions, Genetic - genetics ; Recombinase ; Recombinases ; Recombination ; Temperature ; Temperature dependence ; Temperature modulation of gene expression ; Transcription ; Type 1 fimbriae</subject><ispartof>FEMS microbiology letters, 1998-05, Vol.162 (1), p.17-23</ispartof><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. 1998</rights><rights>1998 INIST-CNRS</rights><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4347-25f3e366c55233484e9c510fb252a12b419e158b44c0d32faf2a21b57364bfd63</citedby><cites>FETCH-LOGICAL-c4347-25f3e366c55233484e9c510fb252a12b419e158b44c0d32faf2a21b57364bfd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1998.tb12973.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1998.tb12973.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2296516$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9595659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olsen, Peter B</creatorcontrib><creatorcontrib>Schembri, Mark A</creatorcontrib><creatorcontrib>Gally, David L</creatorcontrib><creatorcontrib>Klemm, Per</creatorcontrib><title>Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element. This DNA switch directs transcription of fimA, encoding the major type 1 fimbrial subunit, in the on orientation but not in the off orientation. Inversion of the DNA element requires either FimB (confers both on and off orientations) or FimE (confers off orientation). Here we show, by chromosomally located fimB- and fimE-lacZ cassettes in isogenic strain sets differing only in the hns locus, how the global regulator H-NS affects the expression of type 1 fimbriae. H-NS was found to downregulate fimB and fimE in a temperature-dependent manner which affected the genes inversely at 30°C and 37°C. By gel-retardation assays H-NS binding was demonstrated to the regions containing the fimB promoter and the fimE promoter, respectively. In vitro recombination analysis suggested no direct involvement of H-NS in the inversion of the phase switch. Rather than directly affecting the switching process per se, it appeared that the orientation of this element was affected by the differential temperature modulation of H-NS of the fimB and fimE genes. Taken together the results suggest that H-NS modulates expression of type 1 fimbriae in a way which seems to favor a fimbriate state at the mammalian body temperature.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Body temperature</subject><subject>Cassettes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Nucleotidyltransferases - genetics</subject><subject>DNA, Bacterial - metabolism</subject><subject>DNA, Recombinant</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>E coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - physiology</subject><subject>Escherichia coli Proteins</subject><subject>Fimbriae, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial - physiology</subject><subject>Genes</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetics</subject><subject>H‐NS</subject><subject>H‐NS binding</subject><subject>Integrases</subject><subject>Inversion</subject><subject>Microbiology</subject><subject>Modulation</subject><subject>Orientation</subject><subject>Phase variations</subject><subject>Pili</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinase</subject><subject>Recombinases</subject><subject>Recombination</subject><subject>Temperature</subject><subject>Temperature dependence</subject><subject>Temperature modulation of gene expression</subject><subject>Transcription</subject><subject>Type 1 fimbriae</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkV-L1DAUxYso67r6EYSg4lvH3PxtBB903XWFUR_U55BmEidD29SkZXa-hx_Y1inzIIqYlwTu75xzwymKJ4BXMJ0XuxVwyUqhRLUCparVUANRkq5u7xTnp9Hd4hxTWZWAlbxfPMh5hzFmBIuz4kxxxQVX58WPt8F7l1w3BNOgwbW9S2YYk0Nt3IyNGULsUH1AN-XHzyh6NGwd8qF9g0y3mR9XKDkb2zp0Jjv0zXUuo_022C2ysRtSbH4pYgpTwtFsMRkOvUMwW9Rpju63s0Heh8FuHxb3vGmye7TcF8XX66svlzfl-tO795ev16VllMmScE8dFcJyTihlFXPKcsC-JpwYIDUD5YBXNWMWbyjxxhNDoOaSClb7jaAXxfOjb5_i99HlQbchW9c0pnNxzFqqSspK0n-CIKZ8ADyBT38Dd3FM3fQJPa0ITBAFMFEvj5RNMefkvO5TaE06aMB6bljv9FyjnmvUc8N6aVjfTuLHS8RYt25zki6VTvNny9xkaxqfTGdDPmGEKMFh_vurI7YPjTv8xwL6-sMa5KTnR30c-7-oyz-t_xOY6dLl</recordid><startdate>199805</startdate><enddate>199805</enddate><creator>Olsen, Peter B</creator><creator>Schembri, Mark A</creator><creator>Gally, David L</creator><creator>Klemm, Per</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199805</creationdate><title>Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch</title><author>Olsen, Peter B ; Schembri, Mark A ; Gally, David L ; Klemm, Per</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4347-25f3e366c55233484e9c510fb252a12b419e158b44c0d32faf2a21b57364bfd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Body temperature</topic><topic>Cassettes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Nucleotidyltransferases - genetics</topic><topic>DNA, Bacterial - metabolism</topic><topic>DNA, Recombinant</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>E coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - physiology</topic><topic>Escherichia coli Proteins</topic><topic>Fimbriae, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial - physiology</topic><topic>Genes</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetics</topic><topic>H‐NS</topic><topic>H‐NS binding</topic><topic>Integrases</topic><topic>Inversion</topic><topic>Microbiology</topic><topic>Modulation</topic><topic>Orientation</topic><topic>Phase variations</topic><topic>Pili</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinase</topic><topic>Recombinases</topic><topic>Recombination</topic><topic>Temperature</topic><topic>Temperature dependence</topic><topic>Temperature modulation of gene expression</topic><topic>Transcription</topic><topic>Type 1 fimbriae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olsen, Peter B</creatorcontrib><creatorcontrib>Schembri, Mark A</creatorcontrib><creatorcontrib>Gally, David L</creatorcontrib><creatorcontrib>Klemm, Per</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olsen, Peter B</au><au>Schembri, Mark A</au><au>Gally, David L</au><au>Klemm, Per</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1998-05</date><risdate>1998</risdate><volume>162</volume><issue>1</issue><spage>17</spage><epage>23</epage><pages>17-23</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element. This DNA switch directs transcription of fimA, encoding the major type 1 fimbrial subunit, in the on orientation but not in the off orientation. Inversion of the DNA element requires either FimB (confers both on and off orientations) or FimE (confers off orientation). Here we show, by chromosomally located fimB- and fimE-lacZ cassettes in isogenic strain sets differing only in the hns locus, how the global regulator H-NS affects the expression of type 1 fimbriae. H-NS was found to downregulate fimB and fimE in a temperature-dependent manner which affected the genes inversely at 30°C and 37°C. By gel-retardation assays H-NS binding was demonstrated to the regions containing the fimB promoter and the fimE promoter, respectively. In vitro recombination analysis suggested no direct involvement of H-NS in the inversion of the phase switch. Rather than directly affecting the switching process per se, it appeared that the orientation of this element was affected by the differential temperature modulation of H-NS of the fimB and fimE genes. Taken together the results suggest that H-NS modulates expression of type 1 fimbriae in a way which seems to favor a fimbriate state at the mammalian body temperature.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9595659</pmid><doi>10.1111/j.1574-6968.1998.tb12973.x</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1998-05, Vol.162 (1), p.17-23 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Bacterial Proteins - genetics Bacteriology Biological and medical sciences Body temperature Cassettes Deoxyribonucleic acid DNA DNA Nucleotidyltransferases - genetics DNA, Bacterial - metabolism DNA, Recombinant DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism E coli Escherichia coli - genetics Escherichia coli - physiology Escherichia coli Proteins Fimbriae, Bacterial - genetics Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial - physiology Genes Genes, Bacterial - genetics Genetics H‐NS H‐NS binding Integrases Inversion Microbiology Modulation Orientation Phase variations Pili Promoter Regions, Genetic - genetics Recombinase Recombinases Recombination Temperature Temperature dependence Temperature modulation of gene expression Transcription Type 1 fimbriae |
| title | Differential temperature modulation by H-NS of the fimB and fimE recombinase genes which control the orientation of the type 1 fimbrial phase switch |
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