Differential expression of c-fos mRNA and Fos protein in the rat brain after restraint stress or pentylenetetrazol-induced seizures
1. c-fos mRNA expression and Fos protein expression were investigated by in situ hybridization and immunohistochemistry after 30 min of forced restraint stress or pentylenetetrazol (PTZ; 64 mg/kg, i.p.)-induced seizures. 2. Forced restraint stress and PTZ-induced seizures generated c-fos mRNA expres...
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| Veröffentlicht in: | Cellular and molecular neurobiology 1998-06, Vol.18 (3), p.339-346 |
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| container_title | Cellular and molecular neurobiology |
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| creator | Del Bel, E A Silveira, M C Graeff, F G Garcia-Cairasco, N Guimarães, F S |
| description | 1. c-fos mRNA expression and Fos protein expression were investigated by in situ hybridization and immunohistochemistry after 30 min of forced restraint stress or pentylenetetrazol (PTZ; 64 mg/kg, i.p.)-induced seizures. 2. Forced restraint stress and PTZ-induced seizures generated c-fos mRNA expression of distinct intensities, but in similar brain regions, including the hippocampus, the amygdala, the piriform cortex, the paraventricular hypothalamic nucleus, the habenula, and parts of the cerebral cortex. 3. The distribution of Fos-like immunoreactivity induced by stress or seizures only partially overlap. No Fos-like expression was found in the hippocampus or the habenula after restraint stress. Nevertheless, both areas presented Fos-like expression after PTZ-induced seizures. 4. Our results support the suggestion that immediate early gene expression in vivo may exhibit both region- and stimulus-specific expression. |
| doi_str_mv | 10.1023/A:1022505232618 |
| format | Article |
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Forced restraint stress and PTZ-induced seizures generated c-fos mRNA expression of distinct intensities, but in similar brain regions, including the hippocampus, the amygdala, the piriform cortex, the paraventricular hypothalamic nucleus, the habenula, and parts of the cerebral cortex. 3. The distribution of Fos-like immunoreactivity induced by stress or seizures only partially overlap. No Fos-like expression was found in the hippocampus or the habenula after restraint stress. Nevertheless, both areas presented Fos-like expression after PTZ-induced seizures. 4. Our results support the suggestion that immediate early gene expression in vivo may exhibit both region- and stimulus-specific expression.</description><identifier>ISSN: 0272-4340</identifier><identifier>DOI: 10.1023/A:1022505232618</identifier><identifier>PMID: 9590563</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Brain - metabolism ; Brain - physiopathology ; Gene Expression Regulation - drug effects ; Genes, fos - drug effects ; Immobilization ; Immunohistochemistry ; In Situ Hybridization ; Male ; Oncogene Proteins v-fos - biosynthesis ; Oncogene Proteins v-fos - genetics ; Pentylenetetrazole - pharmacology ; Rats ; Rats, Wistar ; RNA, Messenger - biosynthesis ; RNA, Messenger - drug effects ; Seizures - chemically induced ; Seizures - genetics ; Seizures - metabolism ; Stress, Physiological - genetics ; Stress, Physiological - metabolism ; Stress, Physiological - physiopathology</subject><ispartof>Cellular and molecular neurobiology, 1998-06, Vol.18 (3), p.339-346</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-190c427a7b05270f3985f1b49b45431ddfdfffdf6e63393bf950343b36d9de323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9590563$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Del Bel, E A</creatorcontrib><creatorcontrib>Silveira, M C</creatorcontrib><creatorcontrib>Graeff, F G</creatorcontrib><creatorcontrib>Garcia-Cairasco, N</creatorcontrib><creatorcontrib>Guimarães, F S</creatorcontrib><title>Differential expression of c-fos mRNA and Fos protein in the rat brain after restraint stress or pentylenetetrazol-induced seizures</title><title>Cellular and molecular neurobiology</title><addtitle>Cell Mol Neurobiol</addtitle><description>1. c-fos mRNA expression and Fos protein expression were investigated by in situ hybridization and immunohistochemistry after 30 min of forced restraint stress or pentylenetetrazol (PTZ; 64 mg/kg, i.p.)-induced seizures. 2. Forced restraint stress and PTZ-induced seizures generated c-fos mRNA expression of distinct intensities, but in similar brain regions, including the hippocampus, the amygdala, the piriform cortex, the paraventricular hypothalamic nucleus, the habenula, and parts of the cerebral cortex. 3. The distribution of Fos-like immunoreactivity induced by stress or seizures only partially overlap. No Fos-like expression was found in the hippocampus or the habenula after restraint stress. Nevertheless, both areas presented Fos-like expression after PTZ-induced seizures. 4. Our results support the suggestion that immediate early gene expression in vivo may exhibit both region- and stimulus-specific expression.</description><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Brain - physiopathology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes, fos - drug effects</subject><subject>Immobilization</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Oncogene Proteins v-fos - biosynthesis</subject><subject>Oncogene Proteins v-fos - genetics</subject><subject>Pentylenetetrazole - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - drug effects</subject><subject>Seizures - chemically induced</subject><subject>Seizures - genetics</subject><subject>Seizures - metabolism</subject><subject>Stress, Physiological - genetics</subject><subject>Stress, Physiological - metabolism</subject><subject>Stress, Physiological - physiopathology</subject><issn>0272-4340</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUD1PwzAQ9QAqpTAzIXliC9g-O6nZqkIBqQIJwRw58VkEpUmwHYl25Y_jiu5Id3p6unfvPgi54OyaMwE3i9sEQjElQOR8fkSmTBQikyDZCTkN4ZMxphlTEzLRSjOVw5T83DXOoccuNqal-D14DKHpO9o7WmeuD3Tz-rygprN0lcjg-4hNR1PED6TeRFp5k5hxET1NzXFPI02YjGjv6ZC8ty12GDHVdn2bNZ0da7Q0YLMbk-yMHDvTBjw_4Iy8r-7flo_Z-uXhablYZzUIHTOuWS1FYYoqXVgwB3quHK-krqSSwK111rmUOeYAGiqnFQMJFeRWWwQBM3L155uu-BrTquWmCTW2remwH0NZ6HmRFxL-FfI8zeZy73h5EI7VBm05-GZj_LY8vBd-ATYifHI</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Del Bel, E A</creator><creator>Silveira, M C</creator><creator>Graeff, F G</creator><creator>Garcia-Cairasco, N</creator><creator>Guimarães, F S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19980601</creationdate><title>Differential expression of c-fos mRNA and Fos protein in the rat brain after restraint stress or pentylenetetrazol-induced seizures</title><author>Del Bel, E A ; 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Forced restraint stress and PTZ-induced seizures generated c-fos mRNA expression of distinct intensities, but in similar brain regions, including the hippocampus, the amygdala, the piriform cortex, the paraventricular hypothalamic nucleus, the habenula, and parts of the cerebral cortex. 3. The distribution of Fos-like immunoreactivity induced by stress or seizures only partially overlap. No Fos-like expression was found in the hippocampus or the habenula after restraint stress. Nevertheless, both areas presented Fos-like expression after PTZ-induced seizures. 4. Our results support the suggestion that immediate early gene expression in vivo may exhibit both region- and stimulus-specific expression.</abstract><cop>Netherlands</cop><pmid>9590563</pmid><doi>10.1023/A:1022505232618</doi><tpages>8</tpages></addata></record> |
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| ispartof | Cellular and molecular neurobiology, 1998-06, Vol.18 (3), p.339-346 |
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| language | eng |
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| subjects | Animals Brain - metabolism Brain - physiopathology Gene Expression Regulation - drug effects Genes, fos - drug effects Immobilization Immunohistochemistry In Situ Hybridization Male Oncogene Proteins v-fos - biosynthesis Oncogene Proteins v-fos - genetics Pentylenetetrazole - pharmacology Rats Rats, Wistar RNA, Messenger - biosynthesis RNA, Messenger - drug effects Seizures - chemically induced Seizures - genetics Seizures - metabolism Stress, Physiological - genetics Stress, Physiological - metabolism Stress, Physiological - physiopathology |
| title | Differential expression of c-fos mRNA and Fos protein in the rat brain after restraint stress or pentylenetetrazol-induced seizures |
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