In vitro and in vivo study of pluripotency in intraspecific hybrid cells obtained by fusion of murine embryonic stem cells with splenocytes

Hypoxanthine phosphoribosyltransferase–deficient (HPRT‐) mouse embryonic stem (ES) cells, HM‐1 cells (genotype XY), were fused with adult female DD/c mouse spleen cells. As a result, a set of HAT‐resistant clones was isolated. Four hybrid clones most similar in morphology and growth characteristics...

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Veröffentlicht in:	Molecular reproduction and development 1998-06, Vol.50 (2), p.128-138
Hauptverfasser:	Matveeva, N.M., Shilov, A.G., Kaftanovskaya, E.M., Maximovsky, L.P., Zhelezova, A.I., Golubitsa, A.N., Bayborodin, S.I., Fokina, M.M., Serov, O.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - metabolism Animals Biomarkers Cell Differentiation Cell Line chimeras Extracellular Matrix Proteins - metabolism Female Hybrid Cells Hypoxanthine Phosphoribosyltransferase - biosynthesis Intermediate Filament Proteins - metabolism Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA mouse pluripotency Spleen Stem Cells - cytology X Chromosome
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