Human cord blood-derived primitive progenitors are enriched in CD34+c-kit- cells: correlation between long-term culture-initiating cells and telomerase expression
We studied the functional characteristics of subpopulations of cord blood-derived CD34+ cells expressing different levels of CD38 and c-kit antigens, using clonal cell culture and long-term culture with allogeneic bone marrow stromal cells or the MS-5 murine stromal cell line to assay long-term cult...
Gespeichert in:
| Veröffentlicht in: | Leukemia 1998-05, Vol.12 (5), p.728-734 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 734 |
|---|---|
| container_issue | 5 |
| container_start_page | 728 |
| container_title | Leukemia |
| container_volume | 12 |
| creator | Sakabe, H Yahata, N Kimura, T Zeng, Z Z Minamiguchi, H Kaneko, H Mori, K J Ohyashiki, K Ohyashiki, J H Toyama, K Abe, T Sonoda, Y |
| description | We studied the functional characteristics of subpopulations of cord blood-derived CD34+ cells expressing different levels of CD38 and c-kit antigens, using clonal cell culture and long-term culture with allogeneic bone marrow stromal cells or the MS-5 murine stromal cell line to assay long-term culture-initiating cells (LTC-IC) in each subpopulation. To investigate the capacity for replication, proliferation, and differentiation of each subpopulation of CD34+ cells, we also studied the correlation between LTC-IC and telomerase activity. After 5 weeks of coculture, LTC-IC accounted for one out of 32 CD34+CD38- cells and one out of 33 CD34+c-kit- cells. In contrast, the frequency of LTC-IC was low in their antigen-positive counterparts (one per 84 CD34+CD38+ cells, one per 90 CD34+c-kit(low) cells, and very low among CD34+c-kit(high) cells). It was noteworthy that some LTC-IC derived from CD34+CD38- as well as CD34+c-kit- cells generated colony-forming cells (CFCs) after up to 9 weeks of coculture. Telomerase activity was consistently low in CD34+CD38- and CD34+c-kit- cells compared to CD38+ or c-kit(high or low) cells, suggesting that CD34+CD38- or c-kit- cells are likely to be more quiescent. These results suggest that the CD34+CD38- and CD34+c-kit- cell populations are primitive stem/progenitor cells, and that the telomerase activity of these cells correlates with their proliferative capacity as well as their stage of differentiation. |
| doi_str_mv | 10.1038/sj.leu.2401001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_79873815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2645694191</sourcerecordid><originalsourceid>FETCH-LOGICAL-p166t-9e9cf3235e4c6c5094b6c6f7e5cc6a49bb129db8f357f4bae42baad30de80f8a3</originalsourceid><addsrcrecordid>eNpdkMtuFDEQRS1EFIbAlh2SJSQ2yBO7_eg2u2h4BClSNrBu-VE9eOi2B9sd4Hf4UjzKrFjVlerUrVuF0CtGt4zy4boctjOs205QRil7gjZM9IpIKdlTtKHD0BOlO_EMPS_l0IDWVJfoUkvNu55t0N_bdTERu5Q9tnNKnnjI4QE8PuawhNpkU2kPMdSUCzYZMMQc3PeGhIh3H7h458iPUAl2MM_l_ckrw2xqSBFbqL8AIp5T3JMKecFuneuagYRmGBoU949z2ESPK8xpgWxKW_L7mKGUZvICXUxmLvDyXK_Qt08fv-5uyd395y-7mztyZEpVokG7iXdcgnDKSaqFVU5NPUjnlBHaWtZpb4eJy34S1oDorDGeUw8DnQbDr9DbR992788VSh2XUE7ZTIS0lrHXQ88HJhv45j_wkNYcW7axU0IqLZhmjXp9pla7gB9P_zT5z3h-Pf8HymeKSg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2645694191</pqid></control><display><type>article</type><title>Human cord blood-derived primitive progenitors are enriched in CD34+c-kit- cells: correlation between long-term culture-initiating cells and telomerase expression</title><source>MEDLINE</source><source>Nature</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>SpringerLink Journals - AutoHoldings</source><creator>Sakabe, H ; Yahata, N ; Kimura, T ; Zeng, Z Z ; Minamiguchi, H ; Kaneko, H ; Mori, K J ; Ohyashiki, K ; Ohyashiki, J H ; Toyama, K ; Abe, T ; Sonoda, Y</creator><creatorcontrib>Sakabe, H ; Yahata, N ; Kimura, T ; Zeng, Z Z ; Minamiguchi, H ; Kaneko, H ; Mori, K J ; Ohyashiki, K ; Ohyashiki, J H ; Toyama, K ; Abe, T ; Sonoda, Y</creatorcontrib><description>We studied the functional characteristics of subpopulations of cord blood-derived CD34+ cells expressing different levels of CD38 and c-kit antigens, using clonal cell culture and long-term culture with allogeneic bone marrow stromal cells or the MS-5 murine stromal cell line to assay long-term culture-initiating cells (LTC-IC) in each subpopulation. To investigate the capacity for replication, proliferation, and differentiation of each subpopulation of CD34+ cells, we also studied the correlation between LTC-IC and telomerase activity. After 5 weeks of coculture, LTC-IC accounted for one out of 32 CD34+CD38- cells and one out of 33 CD34+c-kit- cells. In contrast, the frequency of LTC-IC was low in their antigen-positive counterparts (one per 84 CD34+CD38+ cells, one per 90 CD34+c-kit(low) cells, and very low among CD34+c-kit(high) cells). It was noteworthy that some LTC-IC derived from CD34+CD38- as well as CD34+c-kit- cells generated colony-forming cells (CFCs) after up to 9 weeks of coculture. Telomerase activity was consistently low in CD34+CD38- and CD34+c-kit- cells compared to CD38+ or c-kit(high or low) cells, suggesting that CD34+CD38- or c-kit- cells are likely to be more quiescent. These results suggest that the CD34+CD38- and CD34+c-kit- cell populations are primitive stem/progenitor cells, and that the telomerase activity of these cells correlates with their proliferative capacity as well as their stage of differentiation.</description><identifier>ISSN: 0887-6924</identifier><identifier>EISSN: 1476-5551</identifier><identifier>DOI: 10.1038/sj.leu.2401001</identifier><identifier>PMID: 9593271</identifier><language>eng</language><publisher>England: Nature Publishing Group</publisher><subject>Animals ; Antigens ; Antigens, CD34 - biosynthesis ; Antigens, CD34 - blood ; Antigens, Differentiation - biosynthesis ; Blood ; Bone marrow ; c-Kit protein ; CD34 antigen ; CD38 antigen ; Cell culture ; Cell proliferation ; Cells (biology) ; Cells, Cultured ; Chlorofluorocarbons ; CHO Cells - metabolism ; Colony-forming cells ; Cord blood ; Cricetinae ; Differentiation ; Fetal Blood - cytology ; Fetal Blood - enzymology ; Gene Amplification ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - enzymology ; Hemopoiesis ; Humans ; Polymerase Chain Reaction ; Progenitor cells ; Proto-Oncogene Proteins c-kit - biosynthesis ; Proto-Oncogene Proteins c-kit - blood ; Sensitivity and Specificity ; Stromal cells ; Subpopulations ; Telomerase ; Telomerase - biosynthesis ; Telomere ; Time Factors</subject><ispartof>Leukemia, 1998-05, Vol.12 (5), p.728-734</ispartof><rights>Macmillan Publishers Limited 1998.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9593271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakabe, H</creatorcontrib><creatorcontrib>Yahata, N</creatorcontrib><creatorcontrib>Kimura, T</creatorcontrib><creatorcontrib>Zeng, Z Z</creatorcontrib><creatorcontrib>Minamiguchi, H</creatorcontrib><creatorcontrib>Kaneko, H</creatorcontrib><creatorcontrib>Mori, K J</creatorcontrib><creatorcontrib>Ohyashiki, K</creatorcontrib><creatorcontrib>Ohyashiki, J H</creatorcontrib><creatorcontrib>Toyama, K</creatorcontrib><creatorcontrib>Abe, T</creatorcontrib><creatorcontrib>Sonoda, Y</creatorcontrib><title>Human cord blood-derived primitive progenitors are enriched in CD34+c-kit- cells: correlation between long-term culture-initiating cells and telomerase expression</title><title>Leukemia</title><addtitle>Leukemia</addtitle><description>We studied the functional characteristics of subpopulations of cord blood-derived CD34+ cells expressing different levels of CD38 and c-kit antigens, using clonal cell culture and long-term culture with allogeneic bone marrow stromal cells or the MS-5 murine stromal cell line to assay long-term culture-initiating cells (LTC-IC) in each subpopulation. To investigate the capacity for replication, proliferation, and differentiation of each subpopulation of CD34+ cells, we also studied the correlation between LTC-IC and telomerase activity. After 5 weeks of coculture, LTC-IC accounted for one out of 32 CD34+CD38- cells and one out of 33 CD34+c-kit- cells. In contrast, the frequency of LTC-IC was low in their antigen-positive counterparts (one per 84 CD34+CD38+ cells, one per 90 CD34+c-kit(low) cells, and very low among CD34+c-kit(high) cells). It was noteworthy that some LTC-IC derived from CD34+CD38- as well as CD34+c-kit- cells generated colony-forming cells (CFCs) after up to 9 weeks of coculture. Telomerase activity was consistently low in CD34+CD38- and CD34+c-kit- cells compared to CD38+ or c-kit(high or low) cells, suggesting that CD34+CD38- or c-kit- cells are likely to be more quiescent. These results suggest that the CD34+CD38- and CD34+c-kit- cell populations are primitive stem/progenitor cells, and that the telomerase activity of these cells correlates with their proliferative capacity as well as their stage of differentiation.</description><subject>Animals</subject><subject>Antigens</subject><subject>Antigens, CD34 - biosynthesis</subject><subject>Antigens, CD34 - blood</subject><subject>Antigens, Differentiation - biosynthesis</subject><subject>Blood</subject><subject>Bone marrow</subject><subject>c-Kit protein</subject><subject>CD34 antigen</subject><subject>CD38 antigen</subject><subject>Cell culture</subject><subject>Cell proliferation</subject><subject>Cells (biology)</subject><subject>Cells, Cultured</subject><subject>Chlorofluorocarbons</subject><subject>CHO Cells - metabolism</subject><subject>Colony-forming cells</subject><subject>Cord blood</subject><subject>Cricetinae</subject><subject>Differentiation</subject><subject>Fetal Blood - cytology</subject><subject>Fetal Blood - enzymology</subject><subject>Gene Amplification</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - enzymology</subject><subject>Hemopoiesis</subject><subject>Humans</subject><subject>Polymerase Chain Reaction</subject><subject>Progenitor cells</subject><subject>Proto-Oncogene Proteins c-kit - biosynthesis</subject><subject>Proto-Oncogene Proteins c-kit - blood</subject><subject>Sensitivity and Specificity</subject><subject>Stromal cells</subject><subject>Subpopulations</subject><subject>Telomerase</subject><subject>Telomerase - biosynthesis</subject><subject>Telomere</subject><subject>Time Factors</subject><issn>0887-6924</issn><issn>1476-5551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkMtuFDEQRS1EFIbAlh2SJSQ2yBO7_eg2u2h4BClSNrBu-VE9eOi2B9sd4Hf4UjzKrFjVlerUrVuF0CtGt4zy4boctjOs205QRil7gjZM9IpIKdlTtKHD0BOlO_EMPS_l0IDWVJfoUkvNu55t0N_bdTERu5Q9tnNKnnjI4QE8PuawhNpkU2kPMdSUCzYZMMQc3PeGhIh3H7h458iPUAl2MM_l_ckrw2xqSBFbqL8AIp5T3JMKecFuneuagYRmGBoU949z2ESPK8xpgWxKW_L7mKGUZvICXUxmLvDyXK_Qt08fv-5uyd395y-7mztyZEpVokG7iXdcgnDKSaqFVU5NPUjnlBHaWtZpb4eJy34S1oDorDGeUw8DnQbDr9DbR992788VSh2XUE7ZTIS0lrHXQ88HJhv45j_wkNYcW7axU0IqLZhmjXp9pla7gB9P_zT5z3h-Pf8HymeKSg</recordid><startdate>199805</startdate><enddate>199805</enddate><creator>Sakabe, H</creator><creator>Yahata, N</creator><creator>Kimura, T</creator><creator>Zeng, Z Z</creator><creator>Minamiguchi, H</creator><creator>Kaneko, H</creator><creator>Mori, K J</creator><creator>Ohyashiki, K</creator><creator>Ohyashiki, J H</creator><creator>Toyama, K</creator><creator>Abe, T</creator><creator>Sonoda, Y</creator><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199805</creationdate><title>Human cord blood-derived primitive progenitors are enriched in CD34+c-kit- cells: correlation between long-term culture-initiating cells and telomerase expression</title><author>Sakabe, H ; Yahata, N ; Kimura, T ; Zeng, Z Z ; Minamiguchi, H ; Kaneko, H ; Mori, K J ; Ohyashiki, K ; Ohyashiki, J H ; Toyama, K ; Abe, T ; Sonoda, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p166t-9e9cf3235e4c6c5094b6c6f7e5cc6a49bb129db8f357f4bae42baad30de80f8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Antigens, CD34 - biosynthesis</topic><topic>Antigens, CD34 - blood</topic><topic>Antigens, Differentiation - biosynthesis</topic><topic>Blood</topic><topic>Bone marrow</topic><topic>c-Kit protein</topic><topic>CD34 antigen</topic><topic>CD38 antigen</topic><topic>Cell culture</topic><topic>Cell proliferation</topic><topic>Cells (biology)</topic><topic>Cells, Cultured</topic><topic>Chlorofluorocarbons</topic><topic>CHO Cells - metabolism</topic><topic>Colony-forming cells</topic><topic>Cord blood</topic><topic>Cricetinae</topic><topic>Differentiation</topic><topic>Fetal Blood - cytology</topic><topic>Fetal Blood - enzymology</topic><topic>Gene Amplification</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - enzymology</topic><topic>Hemopoiesis</topic><topic>Humans</topic><topic>Polymerase Chain Reaction</topic><topic>Progenitor cells</topic><topic>Proto-Oncogene Proteins c-kit - biosynthesis</topic><topic>Proto-Oncogene Proteins c-kit - blood</topic><topic>Sensitivity and Specificity</topic><topic>Stromal cells</topic><topic>Subpopulations</topic><topic>Telomerase</topic><topic>Telomerase - biosynthesis</topic><topic>Telomere</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakabe, H</creatorcontrib><creatorcontrib>Yahata, N</creatorcontrib><creatorcontrib>Kimura, T</creatorcontrib><creatorcontrib>Zeng, Z Z</creatorcontrib><creatorcontrib>Minamiguchi, H</creatorcontrib><creatorcontrib>Kaneko, H</creatorcontrib><creatorcontrib>Mori, K J</creatorcontrib><creatorcontrib>Ohyashiki, K</creatorcontrib><creatorcontrib>Ohyashiki, J H</creatorcontrib><creatorcontrib>Toyama, K</creatorcontrib><creatorcontrib>Abe, T</creatorcontrib><creatorcontrib>Sonoda, Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Leukemia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakabe, H</au><au>Yahata, N</au><au>Kimura, T</au><au>Zeng, Z Z</au><au>Minamiguchi, H</au><au>Kaneko, H</au><au>Mori, K J</au><au>Ohyashiki, K</au><au>Ohyashiki, J H</au><au>Toyama, K</au><au>Abe, T</au><au>Sonoda, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human cord blood-derived primitive progenitors are enriched in CD34+c-kit- cells: correlation between long-term culture-initiating cells and telomerase expression</atitle><jtitle>Leukemia</jtitle><addtitle>Leukemia</addtitle><date>1998-05</date><risdate>1998</risdate><volume>12</volume><issue>5</issue><spage>728</spage><epage>734</epage><pages>728-734</pages><issn>0887-6924</issn><eissn>1476-5551</eissn><abstract>We studied the functional characteristics of subpopulations of cord blood-derived CD34+ cells expressing different levels of CD38 and c-kit antigens, using clonal cell culture and long-term culture with allogeneic bone marrow stromal cells or the MS-5 murine stromal cell line to assay long-term culture-initiating cells (LTC-IC) in each subpopulation. To investigate the capacity for replication, proliferation, and differentiation of each subpopulation of CD34+ cells, we also studied the correlation between LTC-IC and telomerase activity. After 5 weeks of coculture, LTC-IC accounted for one out of 32 CD34+CD38- cells and one out of 33 CD34+c-kit- cells. In contrast, the frequency of LTC-IC was low in their antigen-positive counterparts (one per 84 CD34+CD38+ cells, one per 90 CD34+c-kit(low) cells, and very low among CD34+c-kit(high) cells). It was noteworthy that some LTC-IC derived from CD34+CD38- as well as CD34+c-kit- cells generated colony-forming cells (CFCs) after up to 9 weeks of coculture. Telomerase activity was consistently low in CD34+CD38- and CD34+c-kit- cells compared to CD38+ or c-kit(high or low) cells, suggesting that CD34+CD38- or c-kit- cells are likely to be more quiescent. These results suggest that the CD34+CD38- and CD34+c-kit- cell populations are primitive stem/progenitor cells, and that the telomerase activity of these cells correlates with their proliferative capacity as well as their stage of differentiation.</abstract><cop>England</cop><pub>Nature Publishing Group</pub><pmid>9593271</pmid><doi>10.1038/sj.leu.2401001</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-6924 |
| ispartof | Leukemia, 1998-05, Vol.12 (5), p.728-734 |
| issn | 0887-6924 1476-5551 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79873815 |
| source | MEDLINE; Nature; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerLink Journals - AutoHoldings |
| subjects | Animals Antigens Antigens, CD34 - biosynthesis Antigens, CD34 - blood Antigens, Differentiation - biosynthesis Blood Bone marrow c-Kit protein CD34 antigen CD38 antigen Cell culture Cell proliferation Cells (biology) Cells, Cultured Chlorofluorocarbons CHO Cells - metabolism Colony-forming cells Cord blood Cricetinae Differentiation Fetal Blood - cytology Fetal Blood - enzymology Gene Amplification Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - enzymology Hemopoiesis Humans Polymerase Chain Reaction Progenitor cells Proto-Oncogene Proteins c-kit - biosynthesis Proto-Oncogene Proteins c-kit - blood Sensitivity and Specificity Stromal cells Subpopulations Telomerase Telomerase - biosynthesis Telomere Time Factors |
| title | Human cord blood-derived primitive progenitors are enriched in CD34+c-kit- cells: correlation between long-term culture-initiating cells and telomerase expression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T03%3A27%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20cord%20blood-derived%20primitive%20progenitors%20are%20enriched%20in%20CD34+c-kit-%20cells:%20correlation%20between%20long-term%20culture-initiating%20cells%20and%20telomerase%20expression&rft.jtitle=Leukemia&rft.au=Sakabe,%20H&rft.date=1998-05&rft.volume=12&rft.issue=5&rft.spage=728&rft.epage=734&rft.pages=728-734&rft.issn=0887-6924&rft.eissn=1476-5551&rft_id=info:doi/10.1038/sj.leu.2401001&rft_dat=%3Cproquest_pubme%3E2645694191%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2645694191&rft_id=info:pmid/9593271&rfr_iscdi=true |