The efficient expression of intravascularly delivered DNA in rat muscle
Previous studies have demonstrated that muscle can take up and express naked DNA or RNA. This study demonstrates that the pDNA can be delivered to and expressed within skeletal muscle when injected rapidly, in a large volume and when all blood vessels leading into and out of the hindlimb are occlude...
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| Veröffentlicht in: | Gene therapy 1998-02, Vol.5 (2), p.272-276 |
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| container_title | Gene therapy |
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| creator | BUDKER, V ZHANG, G DANKO, I WILLIAMS, P WOLFF, J |
| description | Previous studies have demonstrated that muscle can take up and express naked DNA or RNA. This study demonstrates that the pDNA can be delivered to and expressed within skeletal muscle when injected rapidly, in a large volume and when all blood vessels leading into and out of the hindlimb are occluded. The additional use of collagenase, papaverine and ischemia raised expression moderately but was not critical. These results demonstrate that a nonviral method can lead to high levels of expression in the muscles of adult animals large than mice. |
| doi_str_mv | 10.1038/sj.gt.3300572 |
| format | Article |
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Psychology ; Gene Expression ; Gene therapy ; Gene Transfer Techniques ; Genetic Therapy - methods ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Injections, Intramuscular ; Injections, Intravenous ; Ischemia ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - metabolism ; Papaverine - administration & dosage ; Plasmids ; Rats ; Rats, Sprague-Dawley ; Skeletal muscle</subject><ispartof>Gene therapy, 1998-02, Vol.5 (2), p.272-276</ispartof><rights>1998 INIST-CNRS</rights><rights>Macmillan Publishers Limited 1998.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-8699adcbef6c61a38fc19dca7c2bbdd2a3807d923167b05127a6b2dc257cecf73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2160086$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9578848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BUDKER, V</creatorcontrib><creatorcontrib>ZHANG, G</creatorcontrib><creatorcontrib>DANKO, I</creatorcontrib><creatorcontrib>WILLIAMS, P</creatorcontrib><creatorcontrib>WOLFF, J</creatorcontrib><title>The efficient expression of intravascularly delivered DNA in rat muscle</title><title>Gene therapy</title><addtitle>Gene Ther</addtitle><description>Previous studies have demonstrated that muscle can take up and express naked DNA or RNA. 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| fulltext | fulltext |
| identifier | ISSN: 0969-7128 |
| ispartof | Gene therapy, 1998-02, Vol.5 (2), p.272-276 |
| issn | 0969-7128 1476-5462 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79867324 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Biological and medical sciences Biotechnology Blood vessels Collagen Collagenase Collagenases - administration & dosage Deoxyribonucleic acid DNA DNA - administration & dosage Fundamental and applied biological sciences. Psychology Gene Expression Gene therapy Gene Transfer Techniques Genetic Therapy - methods Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Injections, Intramuscular Injections, Intravenous Ischemia Muscle, Skeletal - blood supply Muscle, Skeletal - metabolism Papaverine - administration & dosage Plasmids Rats Rats, Sprague-Dawley Skeletal muscle |
| title | The efficient expression of intravascularly delivered DNA in rat muscle |
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