Mitochondrial Fixation for the Detection of Cytochrome Oxidase Activity Using Microwave Irradiation

We examined cell fixation with microwave irradiation (MWI) used in cytochemistry. MWI was applied to blocks of about 1mm3 of mouse parotid glands at 500W for about 5 sec in a fixative at 37°C. The activities of endogenous peroxidase and mitochondrial cytochrome oxidse were demonstrated by usinng the...

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Veröffentlicht in:	Okajimas Folia Anatomica Japonica 1998/03/20, Vol.74(6), pp.207-215
Hauptverfasser:	MORIGUCHI, Keiichi, UTSUMI, Michiya, OHNO, Norikazu
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cricetinae Cytochemistry Cytochrome oxidase Electron Transport Complex IV - metabolism Liver - cytology Liver - enzymology Male Mesocricetus Mice Mice, Inbred Strains Microscopy, Electron Microwave irradiation Microwaves Mitochondria Mitochondria - enzymology Mitochondria - radiation effects Mitochondria - ultrastructure Parotid Gland - cytology Parotid Gland - enzymology Peroxidase Tissue Fixation - methods
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