A novel combination of prosthetic groups in a fungal laccase; PQQ and two copper atoms

Extracellular laccase (benzenediol:oxygen oxidoreductase EC 1.10.3.2) from the lignin-degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a...

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Veröffentlicht in:	FEBS letters 1990-07, Vol.267 (1), p.6-8
Hauptverfasser:	Karhunen, Eija, Niku-Paavola, Marja-L., Viikari, Liisa, Haltia, Tuomas, van der Meer, Robert A., Duine, Johannis A.
Format:	Artikel
Sprache:	eng
Schlagworte:	AAS, atomic absorption spectrometry ABTS, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) Analytical, structural and metabolic biochemistry Biological and medical sciences copper Copper - analysis Copper enzyme EDTA, ethylenediaminetetraacetic acid Electron Spin Resonance Spectroscopy electron transfer Enzymes and enzyme inhibitors EPR, electron paramagnetic resonance extracellular enzymes Fundamental and applied biological sciences. Psychology Fungi - enzymology GLC-MS, gas liquid chromatography-mass spectrometry IEF, isoelectric focusing Laccase Lignin degradation Oxidoreductases Oxidoreductases - analysis Oxygen - metabolism Phenols - metabolism PQQ PQQ Cofactor PQQ, pyrroloquinoline quinone pyrroloquinoline quinone Quinolones - analysis SDS, sodium dodecyl sulphate White rot fungus
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