A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation
We created a library of DNA molecules in which the required TATA element of a yeast gal-his3 promoter is replaced by random-sequence oligomers averaging 16 bp in length. Surprisingly, 1% of such random sequences functionally replace the native yeast TATA element. In many cases, sequences completely...
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| Veröffentlicht in: | Genes & development 1990-04, Vol.4 (4), p.636-645 |
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| container_title | Genes & development |
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| creator | SINGER, V. L WOBBE, C. R STRUHL, K |
| description | We created a library of DNA molecules in which the required TATA element of a yeast gal-his3 promoter is replaced by random-sequence oligomers averaging 16 bp in length. Surprisingly, 1% of such random sequences functionally replace the native yeast TATA element. In many cases, sequences completely unrelated to the consensus TATA element (TATAAA) stimulate transcription with equal or increased efficiency. Transcription mediated by these synthetic elements requires GAL4 and is initiated from normal his3 initiation sites, suggesting that it occurs by a mechanism indistinguishable from that involving wild-type TATA elements. Many, but not all, of these elements act as substrates for yeast TFIID in reconstituted transcription reactions in vitro. These observations indicate that yeast TFIID can stimulate transcription from TATA elements whose sequences differ from the consensus, and they suggest the possibility of alternative factors that may provide a related function for transcriptional activation. |
| doi_str_mv | 10.1101/gad.4.4.636 |
| format | Article |
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These observations indicate that yeast TFIID can stimulate transcription from TATA elements whose sequences differ from the consensus, and they suggest the possibility of alternative factors that may provide a related function for transcriptional activation.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.4.4.636</identifier><identifier>PMID: 2163345</identifier><identifier>CODEN: GEDEEP</identifier><language>eng</language><publisher>Cold Spring Harbor, NY: Cold Spring Harbor Laboratory</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; DNA Transposable Elements ; DNA, Fungal - genetics ; Fundamental and applied biological sciences. 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L</creatorcontrib><creatorcontrib>WOBBE, C. R</creatorcontrib><creatorcontrib>STRUHL, K</creatorcontrib><title>A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>We created a library of DNA molecules in which the required TATA element of a yeast gal-his3 promoter is replaced by random-sequence oligomers averaging 16 bp in length. Surprisingly, 1% of such random sequences functionally replace the native yeast TATA element. In many cases, sequences completely unrelated to the consensus TATA element (TATAAA) stimulate transcription with equal or increased efficiency. Transcription mediated by these synthetic elements requires GAL4 and is initiated from normal his3 initiation sites, suggesting that it occurs by a mechanism indistinguishable from that involving wild-type TATA elements. Many, but not all, of these elements act as substrates for yeast TFIID in reconstituted transcription reactions in vitro. These observations indicate that yeast TFIID can stimulate transcription from TATA elements whose sequences differ from the consensus, and they suggest the possibility of alternative factors that may provide a related function for transcriptional activation.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>DNA Transposable Elements</subject><subject>DNA, Fungal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions, Genetic</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Transcription Factor TFIID</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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R ; STRUHL, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-7ee8993eb6f8d9c6126cb3726dd0c333bb8edc5a9e98e639fbdd54f68ead39483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>DNA Transposable Elements</topic><topic>DNA, Fungal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions, Genetic</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Transcription Factor TFIID</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SINGER, V. L</creatorcontrib><creatorcontrib>WOBBE, C. 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R</au><au>STRUHL, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1990-04-01</date><risdate>1990</risdate><volume>4</volume><issue>4</issue><spage>636</spage><epage>645</epage><pages>636-645</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><coden>GEDEEP</coden><abstract>We created a library of DNA molecules in which the required TATA element of a yeast gal-his3 promoter is replaced by random-sequence oligomers averaging 16 bp in length. Surprisingly, 1% of such random sequences functionally replace the native yeast TATA element. In many cases, sequences completely unrelated to the consensus TATA element (TATAAA) stimulate transcription with equal or increased efficiency. Transcription mediated by these synthetic elements requires GAL4 and is initiated from normal his3 initiation sites, suggesting that it occurs by a mechanism indistinguishable from that involving wild-type TATA elements. Many, but not all, of these elements act as substrates for yeast TFIID in reconstituted transcription reactions in vitro. These observations indicate that yeast TFIID can stimulate transcription from TATA elements whose sequences differ from the consensus, and they suggest the possibility of alternative factors that may provide a related function for transcriptional activation.</abstract><cop>Cold Spring Harbor, NY</cop><pub>Cold Spring Harbor Laboratory</pub><pmid>2163345</pmid><doi>10.1101/gad.4.4.636</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genes & development, 1990-04, Vol.4 (4), p.636-645 |
| issn | 0890-9369 1549-5477 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Base Sequence Biological and medical sciences Blotting, Northern DNA Transposable Elements DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Molecular Sequence Data Promoter Regions, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Transcription Factor TFIID Transcription Factors - metabolism Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing |
| title | A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation |
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