Activin-like factor from a Xenopus laevis cell line responsible for mesoderm induction
INDUCTION of mesoderm during early amphibian embryogenesis can be mimicked in vitro by adding growth factors, including heparin-binding and type-beta transforming growth factors (TGF-β), to isolated ectoderm expiants from Xenopus laevis embryos 1–5 . Although the mesoderm-inducing factor (MIF) from...
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| Veröffentlicht in: | Nature (London) 1990-06, Vol.345 (6277), p.732-734 |
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| creator | van den Eijnden-Van Raaij, A. J. M. van Zoelent, E. J. J. van Nimmen, K. Koster, C. H. Snoek, G. T. Durston, A. J. Huylebroeck, D. |
| description | INDUCTION of mesoderm during early amphibian embryogenesis can be mimicked
in vitro
by adding growth factors, including heparin-binding and type-beta transforming growth factors (TGF-β), to isolated ectoderm expiants from
Xenopus laevis
embryos
1–5
. Although the mesoderm-inducing factor (MIF) from
X. laevis
XTC cells (XTC-MIF)
6,7
has properties similar to TGF-β, this factor is still unidentified. Recently, we obtained a number of homogeneous cell lines from the heterogeneous XTC population, which differ in their MIF production
8
. Only one, XTC-GTX-11, produced MIF, although it was similar to the rest of the clones in its production of known growth factors, including TGF-β activity
8
. This observation, together with the identification of activin A as a potent MIF (ref. 9) led us to study the parallel activities of MIF and activin. Here we report an analysis of activin-like activity from XTC cells and some of the XTC clones, including XTC-GTX-11. There is a clear consistent correlation between MIF activity and presence of activin activity, indicating that XTC-MIF is the Xenopus homologue of mammalian activin. |
| doi_str_mv | 10.1038/345732a0 |
| format | Article |
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in vitro
by adding growth factors, including heparin-binding and type-beta transforming growth factors (TGF-β), to isolated ectoderm expiants from
Xenopus laevis
embryos
1–5
. Although the mesoderm-inducing factor (MIF) from
X. laevis
XTC cells (XTC-MIF)
6,7
has properties similar to TGF-β, this factor is still unidentified. Recently, we obtained a number of homogeneous cell lines from the heterogeneous XTC population, which differ in their MIF production
8
. Only one, XTC-GTX-11, produced MIF, although it was similar to the rest of the clones in its production of known growth factors, including TGF-β activity
8
. This observation, together with the identification of activin A as a potent MIF (ref. 9) led us to study the parallel activities of MIF and activin. Here we report an analysis of activin-like activity from XTC cells and some of the XTC clones, including XTC-GTX-11. There is a clear consistent correlation between MIF activity and presence of activin activity, indicating that XTC-MIF is the Xenopus homologue of mammalian activin.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/345732a0</identifier><identifier>PMID: 2113616</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Activin ; Activins ; Animals ; Biological and medical sciences ; Biological Assay ; Blastocyst - analysis ; Cell Differentiation - drug effects ; Cell Line ; Cell lines ; Ectoderm ; Embryogenesis ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic growth stage ; Explants ; Follicle Stimulating Hormone - secretion ; Fundamental and applied biological sciences. Psychology ; Growth factors ; Heparin ; Homology ; Humanities and Social Sciences ; Inhibins - analysis ; Inhibins - pharmacology ; letter ; Leukemia, Erythroblastic, Acute - pathology ; Macromolecular Substances ; Mesoderm ; Mesoderm - drug effects ; Mesoderm - physiology ; Molecular embryology ; Molecular Weight ; multidisciplinary ; Pituitary Gland, Anterior - drug effects ; Pituitary Gland, Anterior - secretion ; Rats ; Recombinant Proteins - pharmacology ; Science ; Science (multidisciplinary) ; Transforming Growth Factors - pharmacology ; Xenopus laevis ; Xenopus laevis - embryology</subject><ispartof>Nature (London), 1990-06, Vol.345 (6277), p.732-734</ispartof><rights>Springer Nature Limited 1990</rights><rights>1991 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jun 21, 1990</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-718e50f277f7e8adab1601243e80adfb59bc962ec2d1a33d81f1a436b23cec453</citedby><cites>FETCH-LOGICAL-c436t-718e50f277f7e8adab1601243e80adfb59bc962ec2d1a33d81f1a436b23cec453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/345732a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/345732a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19294054$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2113616$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van den Eijnden-Van Raaij, A. J. M.</creatorcontrib><creatorcontrib>van Zoelent, E. J. J.</creatorcontrib><creatorcontrib>van Nimmen, K.</creatorcontrib><creatorcontrib>Koster, C. H.</creatorcontrib><creatorcontrib>Snoek, G. T.</creatorcontrib><creatorcontrib>Durston, A. J.</creatorcontrib><creatorcontrib>Huylebroeck, D.</creatorcontrib><title>Activin-like factor from a Xenopus laevis cell line responsible for mesoderm induction</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>INDUCTION of mesoderm during early amphibian embryogenesis can be mimicked
in vitro
by adding growth factors, including heparin-binding and type-beta transforming growth factors (TGF-β), to isolated ectoderm expiants from
Xenopus laevis
embryos
1–5
. Although the mesoderm-inducing factor (MIF) from
X. laevis
XTC cells (XTC-MIF)
6,7
has properties similar to TGF-β, this factor is still unidentified. Recently, we obtained a number of homogeneous cell lines from the heterogeneous XTC population, which differ in their MIF production
8
. Only one, XTC-GTX-11, produced MIF, although it was similar to the rest of the clones in its production of known growth factors, including TGF-β activity
8
. This observation, together with the identification of activin A as a potent MIF (ref. 9) led us to study the parallel activities of MIF and activin. Here we report an analysis of activin-like activity from XTC cells and some of the XTC clones, including XTC-GTX-11. There is a clear consistent correlation between MIF activity and presence of activin activity, indicating that XTC-MIF is the Xenopus homologue of mammalian activin.</description><subject>Activin</subject><subject>Activins</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Blastocyst - analysis</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Ectoderm</subject><subject>Embryogenesis</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic growth stage</subject><subject>Explants</subject><subject>Follicle Stimulating Hormone - secretion</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth factors</subject><subject>Heparin</subject><subject>Homology</subject><subject>Humanities and Social Sciences</subject><subject>Inhibins - analysis</subject><subject>Inhibins - pharmacology</subject><subject>letter</subject><subject>Leukemia, Erythroblastic, Acute - pathology</subject><subject>Macromolecular Substances</subject><subject>Mesoderm</subject><subject>Mesoderm - drug effects</subject><subject>Mesoderm - physiology</subject><subject>Molecular embryology</subject><subject>Molecular Weight</subject><subject>multidisciplinary</subject><subject>Pituitary Gland, Anterior - drug effects</subject><subject>Pituitary Gland, Anterior - secretion</subject><subject>Rats</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Transforming Growth Factors - pharmacology</subject><subject>Xenopus laevis</subject><subject>Xenopus laevis - embryology</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNplkM1LHTEUxUNp0Vct9B8oBIqlXYy9-ZgksxTphyC40dLdkMnclOhM8kzeCP73zeO9VtDVXZzfOfdwCHnP4JSBMF-FbLXgFl6RFZNaNVIZ_ZqsALhpwAh1SN6WcgsALdPygBxwxoRiakV-nblNeAixmcIdUm_dJmXqc5qppb8xpvVS6GTxIRTqcJroFCLSjGWdYgnDVC2Vn7GkEfNMQxyXmpfiMXnj7VTw3f4ekZvv367PfzaXVz8uzs8uGyeF2jSaGWzBc629RmNHOzAFjEuBBuzoh7YbXKc4Oj4yK8RomGe2OgcuHDrZiiPyaZe7zul-wbLp51C2RW3EtJRed6Zl0EEFPz4Db9OSY-3Wcy5A6a6DLfV5R7mcSsno-3UOs82PPYN-O3T_b-iKftgHLsOM439wv2zVT_a6Lc5OPtvoQnnK63gnoZWV-7LjSpXiH8xPxV78_AsT2JGL</recordid><startdate>19900621</startdate><enddate>19900621</enddate><creator>van den Eijnden-Van Raaij, A. 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J. M. ; van Zoelent, E. J. J. ; van Nimmen, K. ; Koster, C. H. ; Snoek, G. T. ; Durston, A. J. ; Huylebroeck, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-718e50f277f7e8adab1601243e80adfb59bc962ec2d1a33d81f1a436b23cec453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Activin</topic><topic>Activins</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Blastocyst - analysis</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Ectoderm</topic><topic>Embryogenesis</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic growth stage</topic><topic>Explants</topic><topic>Follicle Stimulating Hormone - secretion</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth factors</topic><topic>Heparin</topic><topic>Homology</topic><topic>Humanities and Social Sciences</topic><topic>Inhibins - analysis</topic><topic>Inhibins - pharmacology</topic><topic>letter</topic><topic>Leukemia, Erythroblastic, Acute - pathology</topic><topic>Macromolecular Substances</topic><topic>Mesoderm</topic><topic>Mesoderm - drug effects</topic><topic>Mesoderm - physiology</topic><topic>Molecular embryology</topic><topic>Molecular Weight</topic><topic>multidisciplinary</topic><topic>Pituitary Gland, Anterior - drug effects</topic><topic>Pituitary Gland, Anterior - secretion</topic><topic>Rats</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Transforming Growth Factors - pharmacology</topic><topic>Xenopus laevis</topic><topic>Xenopus laevis - embryology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van den Eijnden-Van Raaij, A. 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J. M.</au><au>van Zoelent, E. J. J.</au><au>van Nimmen, K.</au><au>Koster, C. H.</au><au>Snoek, G. T.</au><au>Durston, A. J.</au><au>Huylebroeck, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activin-like factor from a Xenopus laevis cell line responsible for mesoderm induction</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1990-06-21</date><risdate>1990</risdate><volume>345</volume><issue>6277</issue><spage>732</spage><epage>734</epage><pages>732-734</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>INDUCTION of mesoderm during early amphibian embryogenesis can be mimicked
in vitro
by adding growth factors, including heparin-binding and type-beta transforming growth factors (TGF-β), to isolated ectoderm expiants from
Xenopus laevis
embryos
1–5
. Although the mesoderm-inducing factor (MIF) from
X. laevis
XTC cells (XTC-MIF)
6,7
has properties similar to TGF-β, this factor is still unidentified. Recently, we obtained a number of homogeneous cell lines from the heterogeneous XTC population, which differ in their MIF production
8
. Only one, XTC-GTX-11, produced MIF, although it was similar to the rest of the clones in its production of known growth factors, including TGF-β activity
8
. This observation, together with the identification of activin A as a potent MIF (ref. 9) led us to study the parallel activities of MIF and activin. Here we report an analysis of activin-like activity from XTC cells and some of the XTC clones, including XTC-GTX-11. There is a clear consistent correlation between MIF activity and presence of activin activity, indicating that XTC-MIF is the Xenopus homologue of mammalian activin.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>2113616</pmid><doi>10.1038/345732a0</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1990-06, Vol.345 (6277), p.732-734 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79851090 |
| source | MEDLINE; SpringerLink Journals; Nature |
| subjects | Activin Activins Animals Biological and medical sciences Biological Assay Blastocyst - analysis Cell Differentiation - drug effects Cell Line Cell lines Ectoderm Embryogenesis Embryology: invertebrates and vertebrates. Teratology Embryonic growth stage Explants Follicle Stimulating Hormone - secretion Fundamental and applied biological sciences. Psychology Growth factors Heparin Homology Humanities and Social Sciences Inhibins - analysis Inhibins - pharmacology letter Leukemia, Erythroblastic, Acute - pathology Macromolecular Substances Mesoderm Mesoderm - drug effects Mesoderm - physiology Molecular embryology Molecular Weight multidisciplinary Pituitary Gland, Anterior - drug effects Pituitary Gland, Anterior - secretion Rats Recombinant Proteins - pharmacology Science Science (multidisciplinary) Transforming Growth Factors - pharmacology Xenopus laevis Xenopus laevis - embryology |
| title | Activin-like factor from a Xenopus laevis cell line responsible for mesoderm induction |
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