Immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of Brucella ovis
Brucella ovis rough lipopolysaccharide (R-LPS) was studied with respect to its heterogeneity, chain length, sugar composition and immunological activity. R-LPS was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. Gel-filtration of the...
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| Veröffentlicht in: | Veterinary microbiology 1990-05, Vol.22 (4), p.329-334 |
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| creator | Suarez, Carlos E. Pacheco, Gabriel A. Vigliocco, Ana M. |
| description | Brucella ovis rough lipopolysaccharide (R-LPS) was studied with respect to its heterogeneity, chain length, sugar composition and immunological activity. R-LPS was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. Gel-filtration of the upper phase in a column of Bio-Gel P-2 yielded oligosaccharides of 2, 4, 6 and 7 monosaccharides units, 2-keto-deoxy-octulosonic acid (KDO), and monosaccharides. Strong acid hydrolysis followed by paper chromatography showed that the hexa- and heptasaccharides are both composed of glucose, KDO and an unidentified sugar while tetrasaccharide is composed of glucose, mannose and glucosamine. These three oligosaccharides were able to inhibit the LPS-antibody reaction in a solid phase radioimmunoassay, suggesting the oligosaccharides bear antigenic determinants of LPS. |
| doi_str_mv | 10.1016/0378-1135(90)90019-R |
| format | Article |
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R-LPS was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. Gel-filtration of the upper phase in a column of Bio-Gel P-2 yielded oligosaccharides of 2, 4, 6 and 7 monosaccharides units, 2-keto-deoxy-octulosonic acid (KDO), and monosaccharides. Strong acid hydrolysis followed by paper chromatography showed that the hexa- and heptasaccharides are both composed of glucose, KDO and an unidentified sugar while tetrasaccharide is composed of glucose, mannose and glucosamine. These three oligosaccharides were able to inhibit the LPS-antibody reaction in a solid phase radioimmunoassay, suggesting the oligosaccharides bear antigenic determinants of LPS.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/0378-1135(90)90019-R</identifier><identifier>PMID: 2363245</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Bacteriology ; Biological and medical sciences ; BRUCELLA ; Brucella - analysis ; Brucella - immunology ; Chromatography, Gel ; Chromatography, Paper ; DIAGNOSIS ; DIAGNOSTIC ; DIAGNOSTICO ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; Immunohistochemistry ; IMMUNOLOGICAL TECHNIQUES ; LIPOPOLISACARIDOS ; LIPOPOLYSACCHARIDE ; LIPOPOLYSACCHARIDES ; Lipopolysaccharides - analysis ; Lipopolysaccharides - immunology ; Microbiology ; Morphology, structure, chemical composition ; Oligosaccharides - analysis ; Oligosaccharides - immunology ; OVIN ; OVINOS ; Radioimmunoassay ; SHEEP ; TECHNIQUE IMMUNOLOGIQUE ; TECNICAS INMUNOLOGICAS</subject><ispartof>Veterinary microbiology, 1990-05, Vol.22 (4), p.329-334</ispartof><rights>1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-ef482b98ef331d05c9e45e6baa5e4ba50ce9efb045f28f772a047c66a55d71323</citedby><cites>FETCH-LOGICAL-c436t-ef482b98ef331d05c9e45e6baa5e4ba50ce9efb045f28f772a047c66a55d71323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/037811359090019R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6896698$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2363245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suarez, Carlos E.</creatorcontrib><creatorcontrib>Pacheco, Gabriel A.</creatorcontrib><creatorcontrib>Vigliocco, Ana M.</creatorcontrib><title>Immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of Brucella ovis</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Brucella ovis rough lipopolysaccharide (R-LPS) was studied with respect to its heterogeneity, chain length, sugar composition and immunological activity. R-LPS was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. Gel-filtration of the upper phase in a column of Bio-Gel P-2 yielded oligosaccharides of 2, 4, 6 and 7 monosaccharides units, 2-keto-deoxy-octulosonic acid (KDO), and monosaccharides. Strong acid hydrolysis followed by paper chromatography showed that the hexa- and heptasaccharides are both composed of glucose, KDO and an unidentified sugar while tetrasaccharide is composed of glucose, mannose and glucosamine. These three oligosaccharides were able to inhibit the LPS-antibody reaction in a solid phase radioimmunoassay, suggesting the oligosaccharides bear antigenic determinants of LPS.</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>BRUCELLA</subject><subject>Brucella - analysis</subject><subject>Brucella - immunology</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Paper</subject><subject>DIAGNOSIS</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTICO</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Immunohistochemistry</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>LIPOPOLISACARIDOS</subject><subject>LIPOPOLYSACCHARIDE</subject><subject>LIPOPOLYSACCHARIDES</subject><subject>Lipopolysaccharides - analysis</subject><subject>Lipopolysaccharides - immunology</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition</subject><subject>Oligosaccharides - analysis</subject><subject>Oligosaccharides - immunology</subject><subject>OVIN</subject><subject>OVINOS</subject><subject>Radioimmunoassay</subject><subject>SHEEP</subject><subject>TECHNIQUE IMMUNOLOGIQUE</subject><subject>TECNICAS INMUNOLOGICAS</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFDEUhkNR6tr2DxSFuRDRi6n5nuRG0OJHYVEoep1mMifdlJnJmswU-u_NdJf1rl4F8j7ng-cg9IrgC4KJ_IBZo2pCmHin8XuNMdH19RFaEdWwmgpOn6HVAXmBXuZ8hzHmWuJjdEyZZJSLFbq5GoZ5jG4DQ3C2r_I0dwFyFX0V-3Abs3VuY1Polr92smGErvIpDtW0gaoP27iN_cM_ain8nGYHfW-reB_yKXrubZ_hbP-eoN9fv_y6_F6vf367uvy0rh1ncqrBc0VbrcAzRjosnAYuQLbWCuCtFdiBBt9iLjxVvmmoxbxxUlohuoYwyk7Q213fbYp_ZsiTGUJ-XGOEOGfTaCWw1Pq_IBGNKt5kAfkOdCnmnMCbbQqDTQ-GYLNcwCx6zaLXaGweL2CuS9nrff-5HaA7FO2Vl_zNPre5GPfJji7kAyaVllKrgp3vMG-jsbepID_WZQYtg0v4cRdCMXofIJnsAowOupDATaaL4ekl_wKhKquO</recordid><startdate>19900501</startdate><enddate>19900501</enddate><creator>Suarez, Carlos E.</creator><creator>Pacheco, Gabriel A.</creator><creator>Vigliocco, Ana M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19900501</creationdate><title>Immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of Brucella ovis</title><author>Suarez, Carlos E. ; Pacheco, Gabriel A. ; Vigliocco, Ana M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-ef482b98ef331d05c9e45e6baa5e4ba50ce9efb045f28f772a047c66a55d71323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>BRUCELLA</topic><topic>Brucella - analysis</topic><topic>Brucella - immunology</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Paper</topic><topic>DIAGNOSIS</topic><topic>DIAGNOSTIC</topic><topic>DIAGNOSTICO</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>Immunohistochemistry</topic><topic>IMMUNOLOGICAL TECHNIQUES</topic><topic>LIPOPOLISACARIDOS</topic><topic>LIPOPOLYSACCHARIDE</topic><topic>LIPOPOLYSACCHARIDES</topic><topic>Lipopolysaccharides - analysis</topic><topic>Lipopolysaccharides - immunology</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition</topic><topic>Oligosaccharides - analysis</topic><topic>Oligosaccharides - immunology</topic><topic>OVIN</topic><topic>OVINOS</topic><topic>Radioimmunoassay</topic><topic>SHEEP</topic><topic>TECHNIQUE IMMUNOLOGIQUE</topic><topic>TECNICAS INMUNOLOGICAS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suarez, Carlos E.</creatorcontrib><creatorcontrib>Pacheco, Gabriel A.</creatorcontrib><creatorcontrib>Vigliocco, Ana M.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suarez, Carlos E.</au><au>Pacheco, Gabriel A.</au><au>Vigliocco, Ana M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of Brucella ovis</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1990-05-01</date><risdate>1990</risdate><volume>22</volume><issue>4</issue><spage>329</spage><epage>334</epage><pages>329-334</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Brucella ovis rough lipopolysaccharide (R-LPS) was studied with respect to its heterogeneity, chain length, sugar composition and immunological activity. R-LPS was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. Gel-filtration of the upper phase in a column of Bio-Gel P-2 yielded oligosaccharides of 2, 4, 6 and 7 monosaccharides units, 2-keto-deoxy-octulosonic acid (KDO), and monosaccharides. Strong acid hydrolysis followed by paper chromatography showed that the hexa- and heptasaccharides are both composed of glucose, KDO and an unidentified sugar while tetrasaccharide is composed of glucose, mannose and glucosamine. These three oligosaccharides were able to inhibit the LPS-antibody reaction in a solid phase radioimmunoassay, suggesting the oligosaccharides bear antigenic determinants of LPS.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2363245</pmid><doi>10.1016/0378-1135(90)90019-R</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Bacteriology Biological and medical sciences BRUCELLA Brucella - analysis Brucella - immunology Chromatography, Gel Chromatography, Paper DIAGNOSIS DIAGNOSTIC DIAGNOSTICO Fundamental and applied biological sciences. Psychology Hydrolysis Immunohistochemistry IMMUNOLOGICAL TECHNIQUES LIPOPOLISACARIDOS LIPOPOLYSACCHARIDE LIPOPOLYSACCHARIDES Lipopolysaccharides - analysis Lipopolysaccharides - immunology Microbiology Morphology, structure, chemical composition Oligosaccharides - analysis Oligosaccharides - immunology OVIN OVINOS Radioimmunoassay SHEEP TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS |
| title | Immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of Brucella ovis |
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