A microassay to quantitate collagen synthesis by cells in culture

A method to quantitate collagen synthesis, total protein synthesis, and DNA in 24-well culture plates is presented. Collagen-producing cells such as human intestinal smooth muscle cells and dermal fibroblasts were pulse-labeled with [ 3H]proline. After incubation, the plates were heated to 90°C to s...

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Veröffentlicht in:	Analytical biochemistry 1990-05, Vol.186 (2), p.296-300
Hauptverfasser:	Diegelmann, R.F., Bryson, G.R., Flood, L.C., Graham, M.F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Cells, Cultured Collagen - biosynthesis DNA - biosynthesis fibroblasts Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Glycoproteins Humans intestine Intestines - cytology Microbial Collagenase Microchemistry Muscle, Smooth - cytology Muscle, Smooth - metabolism Proline - analysis Protein Biosynthesis Proteins Skin smooth muscle
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