Histochemical and immunocytochemical evaluation of copper and metallothionein in the liver and kidney of copper-loaded rats
Histochemical methods do not always show a good correlation with analytical measurement of copper content and consequently immunoreactive staining techniques for metaliothionein (MT) have recently been employed for the differential diagnosis of copper‐associated diseases. This study compares histoch...
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| Veröffentlicht in: | The Journal of pathology 1990-04, Vol.160 (4), p.305-312 |
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| container_title | The Journal of pathology |
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| creator | Evering, Winston E. Haywood, Susan Elmes, Margaret E. Jasani, Bharat Trafford, James |
| description | Histochemical methods do not always show a good correlation with analytical measurement of copper content and consequently immunoreactive staining techniques for metaliothionein (MT) have recently been employed for the differential diagnosis of copper‐associated diseases. This study compares histochemical with immunocytochemical methods for the assessment of copper status. Male rats were fed a high copper (1 g/kg) diet for 16 weeks and killed sequentially during this period. The livers and kidneys were analysed for copper and zinc (atomic absorption spectrophotometry), and sections were stained with rubeanic acid and rhodanine for copper and for immunoreactive MT using the DNP localization system. Immunoreactive stains for MT corresponded better with copper content than histochemical stains and were more sensitive, albeit less selective, indicators of copper accumulation. Moreover, major differences in intracellular staining were apparent between the two methods, attributed to differences in copper binding and microcompartmentalization of metal. |
| doi_str_mv | 10.1002/path.1711600406 |
| format | Article |
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This study compares histochemical with immunocytochemical methods for the assessment of copper status. Male rats were fed a high copper (1 g/kg) diet for 16 weeks and killed sequentially during this period. The livers and kidneys were analysed for copper and zinc (atomic absorption spectrophotometry), and sections were stained with rubeanic acid and rhodanine for copper and for immunoreactive MT using the DNP localization system. Immunoreactive stains for MT corresponded better with copper content than histochemical stains and were more sensitive, albeit less selective, indicators of copper accumulation. Moreover, major differences in intracellular staining were apparent between the two methods, attributed to differences in copper binding and microcompartmentalization of metal.</description><identifier>ISSN: 0022-3417</identifier><identifier>EISSN: 1096-9896</identifier><identifier>DOI: 10.1002/path.1711600406</identifier><identifier>PMID: 2358968</identifier><identifier>CODEN: JPTLAS</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Biological and medical sciences ; Copper ; Copper - administration & dosage ; Copper - analysis ; Diet ; histochemistry ; Histocytochemistry ; immunocytochemistry ; Kidney Cortex - analysis ; liver ; Liver - analysis ; Male ; Medical sciences ; Metabolic diseases ; metallothionein ; Metallothionein - analysis ; Metals (hemochromatosis...) ; Other metabolic disorders ; Rats ; Rats, Inbred Strains</subject><ispartof>The Journal of pathology, 1990-04, Vol.160 (4), p.305-312</ispartof><rights>Copyright © 1990 John Wiley & Sons, Ltd.</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4426-4c146e3faa9cb45a97b266762cf631b07baf3ed0e55d96bb928d72fd62e75b463</citedby><cites>FETCH-LOGICAL-c4426-4c146e3faa9cb45a97b266762cf631b07baf3ed0e55d96bb928d72fd62e75b463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpath.1711600406$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpath.1711600406$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6850563$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2358968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Evering, Winston E.</creatorcontrib><creatorcontrib>Haywood, Susan</creatorcontrib><creatorcontrib>Elmes, Margaret E.</creatorcontrib><creatorcontrib>Jasani, Bharat</creatorcontrib><creatorcontrib>Trafford, James</creatorcontrib><title>Histochemical and immunocytochemical evaluation of copper and metallothionein in the liver and kidney of copper-loaded rats</title><title>The Journal of pathology</title><addtitle>J. Pathol</addtitle><description>Histochemical methods do not always show a good correlation with analytical measurement of copper content and consequently immunoreactive staining techniques for metaliothionein (MT) have recently been employed for the differential diagnosis of copper‐associated diseases. This study compares histochemical with immunocytochemical methods for the assessment of copper status. Male rats were fed a high copper (1 g/kg) diet for 16 weeks and killed sequentially during this period. The livers and kidneys were analysed for copper and zinc (atomic absorption spectrophotometry), and sections were stained with rubeanic acid and rhodanine for copper and for immunoreactive MT using the DNP localization system. Immunoreactive stains for MT corresponded better with copper content than histochemical stains and were more sensitive, albeit less selective, indicators of copper accumulation. Moreover, major differences in intracellular staining were apparent between the two methods, attributed to differences in copper binding and microcompartmentalization of metal.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Copper</subject><subject>Copper - administration & dosage</subject><subject>Copper - analysis</subject><subject>Diet</subject><subject>histochemistry</subject><subject>Histocytochemistry</subject><subject>immunocytochemistry</subject><subject>Kidney Cortex - analysis</subject><subject>liver</subject><subject>Liver - analysis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>metallothionein</subject><subject>Metallothionein - analysis</subject><subject>Metals (hemochromatosis...)</subject><subject>Other metabolic disorders</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEYhoModW09exLmIN6mTTL5McFTqXZXWKqULT2GTOYbNjYzGScztYv_vKk7bPVUCATyPu-XkAehdwSfEozpWW_G7SmRhAiMGRYv0IJgJXJVKvESLRJB84IR-Rq9ifEHxlgpzo_QES14IsoF-r1ycQx2C62zxmemqzPXtlMX7O6fY7g3fjKjC10WmsyGvofhL9vCaLwP4zZF4LosrXELmXf3M3Dn6g52T63cB1NDnQ1mjCfoVWN8hLfzfoxuLr9sLlb5-tvy68X5OreMUZEzS5iAojFG2Ypxo2RFhZCC2kYUpMKyMk0BNQbOayWqStGylrSpBQXJKyaKY_RxP7cfws8J4qhbFy14bzoIU9RSlaxklDwLEi5KTAVN4NketEOIcYBG94NrzbDTBOtHL_rRi37ykhrv59FT1UJ94GcRKf8w5yamH28G01kXD5goOeaiSNinPfbLedg9d6v-fr5Z_feIfN9OzuHh0DbDnRaykFzfXi31cn21IZtrqT8XfwCzLrjm</recordid><startdate>199004</startdate><enddate>199004</enddate><creator>Evering, Winston E.</creator><creator>Haywood, Susan</creator><creator>Elmes, Margaret E.</creator><creator>Jasani, Bharat</creator><creator>Trafford, James</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199004</creationdate><title>Histochemical and immunocytochemical evaluation of copper and metallothionein in the liver and kidney of copper-loaded rats</title><author>Evering, Winston E. ; Haywood, Susan ; Elmes, Margaret E. ; Jasani, Bharat ; Trafford, James</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4426-4c146e3faa9cb45a97b266762cf631b07baf3ed0e55d96bb928d72fd62e75b463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Copper</topic><topic>Copper - administration & dosage</topic><topic>Copper - analysis</topic><topic>Diet</topic><topic>histochemistry</topic><topic>Histocytochemistry</topic><topic>immunocytochemistry</topic><topic>Kidney Cortex - analysis</topic><topic>liver</topic><topic>Liver - analysis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>metallothionein</topic><topic>Metallothionein - analysis</topic><topic>Metals (hemochromatosis...)</topic><topic>Other metabolic disorders</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Evering, Winston E.</creatorcontrib><creatorcontrib>Haywood, Susan</creatorcontrib><creatorcontrib>Elmes, Margaret E.</creatorcontrib><creatorcontrib>Jasani, Bharat</creatorcontrib><creatorcontrib>Trafford, James</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Evering, Winston E.</au><au>Haywood, Susan</au><au>Elmes, Margaret E.</au><au>Jasani, Bharat</au><au>Trafford, James</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histochemical and immunocytochemical evaluation of copper and metallothionein in the liver and kidney of copper-loaded rats</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>1990-04</date><risdate>1990</risdate><volume>160</volume><issue>4</issue><spage>305</spage><epage>312</epage><pages>305-312</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><coden>JPTLAS</coden><abstract>Histochemical methods do not always show a good correlation with analytical measurement of copper content and consequently immunoreactive staining techniques for metaliothionein (MT) have recently been employed for the differential diagnosis of copper‐associated diseases. This study compares histochemical with immunocytochemical methods for the assessment of copper status. Male rats were fed a high copper (1 g/kg) diet for 16 weeks and killed sequentially during this period. The livers and kidneys were analysed for copper and zinc (atomic absorption spectrophotometry), and sections were stained with rubeanic acid and rhodanine for copper and for immunoreactive MT using the DNP localization system. Immunoreactive stains for MT corresponded better with copper content than histochemical stains and were more sensitive, albeit less selective, indicators of copper accumulation. Moreover, major differences in intracellular staining were apparent between the two methods, attributed to differences in copper binding and microcompartmentalization of metal.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>2358968</pmid><doi>10.1002/path.1711600406</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Copper Copper - administration & dosage Copper - analysis Diet histochemistry Histocytochemistry immunocytochemistry Kidney Cortex - analysis liver Liver - analysis Male Medical sciences Metabolic diseases metallothionein Metallothionein - analysis Metals (hemochromatosis...) Other metabolic disorders Rats Rats, Inbred Strains |
| title | Histochemical and immunocytochemical evaluation of copper and metallothionein in the liver and kidney of copper-loaded rats |
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