Bone conditioned medium enhances cell aggregation, cell proliferation and alkaline phosphatase activity in serum-deprived medium
The effect of medium conditioned by whole bone was examined on the activity of a heterogeneous population of rat calvarial cells grown under serum-deprived conditions. Conditioned medium (CM) had pronounced effects on the appearance of cultures within 24–48 hours of addition. This was characterized...
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| Veröffentlicht in: | Bone (New York, N.Y.) N.Y.), 1990, Vol.11 (2), p.121-126 |
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| container_end_page | 126 |
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| container_issue | 2 |
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| container_title | Bone (New York, N.Y.) |
| container_volume | 11 |
| creator | Gordon, M.T. John, J.St Braidman, I.P. Anderson, D.C. |
| description | The effect of medium conditioned by whole bone was examined on the activity of a heterogeneous population of rat calvarial cells grown under serum-deprived conditions. Conditioned medium (CM) had pronounced effects on the appearance of cultures within 24–48 hours of addition. This was characterized by the breakdown of the cell monolayer, rounding of cells and formation of alkaline phosphatase-positive aggregates. Cellular alkaline phosphatase activity was increased compared to controls while acid phosphatase levels were reduced. These aggregates grown in the presence of 10 mM β-glycerophosphate did not show evidence of mineralization. These results show that soluble factors derived from calvarial bone are responsible for cell aggregation, cell proliferation and increase in alkaline phosphatase activity. |
| doi_str_mv | 10.1016/8756-3282(90)90059-8 |
| format | Article |
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Conditioned medium (CM) had pronounced effects on the appearance of cultures within 24–48 hours of addition. This was characterized by the breakdown of the cell monolayer, rounding of cells and formation of alkaline phosphatase-positive aggregates. Cellular alkaline phosphatase activity was increased compared to controls while acid phosphatase levels were reduced. These aggregates grown in the presence of 10 mM β-glycerophosphate did not show evidence of mineralization. These results show that soluble factors derived from calvarial bone are responsible for cell aggregation, cell proliferation and increase in alkaline phosphatase activity.</description><identifier>ISSN: 8756-3282</identifier><identifier>EISSN: 1873-2763</identifier><identifier>DOI: 10.1016/8756-3282(90)90059-8</identifier><identifier>PMID: 2357422</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acid phosphatase ; Acid Phosphatase - metabolism ; Alkaline phosphatase ; Alkaline Phosphatase - metabolism ; Animals ; Blood ; Bone and Bones - cytology ; Bone and Bones - enzymology ; Calcification, Physiologic ; Calvarial cells ; Cell aggregation ; Cell Aggregation - physiology ; Cell Division - physiology ; Cells, Cultured ; Conditioned medium ; Culture Media ; Microscopy, Electron ; Rats ; Rats, Inbred Strains ; Space life sciences ; Thymidine - metabolism</subject><ispartof>Bone (New York, N.Y.), 1990, Vol.11 (2), p.121-126</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-7d58117c57141ac44b4c7ee8d4b270a7deeecb715b143c143fcc80bb0c16a60e3</citedby><cites>FETCH-LOGICAL-c357t-7d58117c57141ac44b4c7ee8d4b270a7deeecb715b143c143fcc80bb0c16a60e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/8756328290900598$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2357422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gordon, M.T.</creatorcontrib><creatorcontrib>John, J.St</creatorcontrib><creatorcontrib>Braidman, I.P.</creatorcontrib><creatorcontrib>Anderson, D.C.</creatorcontrib><title>Bone conditioned medium enhances cell aggregation, cell proliferation and alkaline phosphatase activity in serum-deprived medium</title><title>Bone (New York, N.Y.)</title><addtitle>Bone</addtitle><description>The effect of medium conditioned by whole bone was examined on the activity of a heterogeneous population of rat calvarial cells grown under serum-deprived conditions. Conditioned medium (CM) had pronounced effects on the appearance of cultures within 24–48 hours of addition. This was characterized by the breakdown of the cell monolayer, rounding of cells and formation of alkaline phosphatase-positive aggregates. Cellular alkaline phosphatase activity was increased compared to controls while acid phosphatase levels were reduced. These aggregates grown in the presence of 10 mM β-glycerophosphate did not show evidence of mineralization. These results show that soluble factors derived from calvarial bone are responsible for cell aggregation, cell proliferation and increase in alkaline phosphatase activity.</description><subject>Acid phosphatase</subject><subject>Acid Phosphatase - metabolism</subject><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Blood</subject><subject>Bone and Bones - cytology</subject><subject>Bone and Bones - enzymology</subject><subject>Calcification, Physiologic</subject><subject>Calvarial cells</subject><subject>Cell aggregation</subject><subject>Cell Aggregation - physiology</subject><subject>Cell Division - physiology</subject><subject>Cells, Cultured</subject><subject>Conditioned medium</subject><subject>Culture Media</subject><subject>Microscopy, Electron</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Space life sciences</subject><subject>Thymidine - metabolism</subject><issn>8756-3282</issn><issn>1873-2763</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UMtOwzAQtBColMIfgOQTAomAnTixc0GCipdUiQucLcfetIY8ip1U6o1Px2mrHjlYa-3Ozs4MQueU3FJCszvB0yxKYhFf5eQ6JyTNI3GAxlTwJIp5lhyi8R5yjE68_yKEJDmnIzSKk5SzOB6j38e2AazbxtjOhq_BNRjb1xiahWo0eKyhqrCazx3M1QC52XaWrq1sCW7Tw6oxWFXfqrKBbblo_XKhOuUBK93Zle3W2DbYg-vryMDS2dX-0Ck6KlXl4WxXJ-jz-elj-hrN3l_epg-zSAepXcRNKijlOuWUUaUZK5jmAMKwIuZEcQMAuuA0LShLdHil1oIUBdE0UxmBZIIut7xB-E8PvpO19YMT1UDbe8lzwZigeQCyLVC71nsHpQx6a-XWkhI5BC-HVOWQqsyJ3AQvRVi72PH3RXC2X9olHeb32zkEkysLTnptISRsrAPdSdPa_w_8AaqdlZE</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Gordon, M.T.</creator><creator>John, J.St</creator><creator>Braidman, I.P.</creator><creator>Anderson, D.C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Bone conditioned medium enhances cell aggregation, cell proliferation and alkaline phosphatase activity in serum-deprived medium</title><author>Gordon, M.T. ; John, J.St ; Braidman, I.P. ; Anderson, D.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-7d58117c57141ac44b4c7ee8d4b270a7deeecb715b143c143fcc80bb0c16a60e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Acid phosphatase</topic><topic>Acid Phosphatase - metabolism</topic><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Blood</topic><topic>Bone and Bones - cytology</topic><topic>Bone and Bones - enzymology</topic><topic>Calcification, Physiologic</topic><topic>Calvarial cells</topic><topic>Cell aggregation</topic><topic>Cell Aggregation - physiology</topic><topic>Cell Division - physiology</topic><topic>Cells, Cultured</topic><topic>Conditioned medium</topic><topic>Culture Media</topic><topic>Microscopy, Electron</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Space life sciences</topic><topic>Thymidine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gordon, M.T.</creatorcontrib><creatorcontrib>John, J.St</creatorcontrib><creatorcontrib>Braidman, I.P.</creatorcontrib><creatorcontrib>Anderson, D.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bone (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gordon, M.T.</au><au>John, J.St</au><au>Braidman, I.P.</au><au>Anderson, D.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone conditioned medium enhances cell aggregation, cell proliferation and alkaline phosphatase activity in serum-deprived medium</atitle><jtitle>Bone (New York, N.Y.)</jtitle><addtitle>Bone</addtitle><date>1990</date><risdate>1990</risdate><volume>11</volume><issue>2</issue><spage>121</spage><epage>126</epage><pages>121-126</pages><issn>8756-3282</issn><eissn>1873-2763</eissn><abstract>The effect of medium conditioned by whole bone was examined on the activity of a heterogeneous population of rat calvarial cells grown under serum-deprived conditions. Conditioned medium (CM) had pronounced effects on the appearance of cultures within 24–48 hours of addition. This was characterized by the breakdown of the cell monolayer, rounding of cells and formation of alkaline phosphatase-positive aggregates. Cellular alkaline phosphatase activity was increased compared to controls while acid phosphatase levels were reduced. These aggregates grown in the presence of 10 mM β-glycerophosphate did not show evidence of mineralization. These results show that soluble factors derived from calvarial bone are responsible for cell aggregation, cell proliferation and increase in alkaline phosphatase activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>2357422</pmid><doi>10.1016/8756-3282(90)90059-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 8756-3282 |
| ispartof | Bone (New York, N.Y.), 1990, Vol.11 (2), p.121-126 |
| issn | 8756-3282 1873-2763 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79844819 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acid phosphatase Acid Phosphatase - metabolism Alkaline phosphatase Alkaline Phosphatase - metabolism Animals Blood Bone and Bones - cytology Bone and Bones - enzymology Calcification, Physiologic Calvarial cells Cell aggregation Cell Aggregation - physiology Cell Division - physiology Cells, Cultured Conditioned medium Culture Media Microscopy, Electron Rats Rats, Inbred Strains Space life sciences Thymidine - metabolism |
| title | Bone conditioned medium enhances cell aggregation, cell proliferation and alkaline phosphatase activity in serum-deprived medium |
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