Purification of a Na+/K+ ATPase inhibitor from borderline hypertensives' plasma

Increasing experimental evidences suggest an involvement of an endogenous Na+/K+ ATPase inhibitor in regulating water and electrolytes balance as well as in the pathogenesis of hypertension. However, conflicting results on the nature and the chemical structure of this substance still make it difficu...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 1990-06, Vol.169 (2), p.360-368
Hauptverfasser: BOSCHI, S, BORGHI, C, MUNARINI, A, VITTORIO COSTA, F, ZHUOREN, L, DEVRIES, D, AMBROSIONI, E
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 368
container_issue 2
container_start_page 360
container_title Biochemical and biophysical research communications
container_volume 169
creator BOSCHI, S
BORGHI, C
MUNARINI, A
VITTORIO COSTA, F
ZHUOREN, L
DEVRIES, D
AMBROSIONI, E
description Increasing experimental evidences suggest an involvement of an endogenous Na+/K+ ATPase inhibitor in regulating water and electrolytes balance as well as in the pathogenesis of hypertension. However, conflicting results on the nature and the chemical structure of this substance still make it difficult to understand exactly its physiological mechanism of action. In the present study an attempt was made to purify a Na+/K+ ATPase inhibitor from hypertensives' plasma by solid phase extraction followed by 2 HPLC steps using reverse and normal phase columns. The fractions, from both columns, were able to inhibit Na+/K+ ATPase, 3H-ouabain binding to enzyme, ouabain sensitive 86Rb uptake and pNPPase activity in a manner not affected by boiling. Ultrafiltration experiments demonstrate that inhibitory activity is largely due to a low-molecular weight substance. These findings seem to confirm the presence in hypertensives plasma of a Na+/K+ ATPase inhibitor with some similarities with ouabain.
doi_str_mv 10.1016/0006-291X(90)90340-S
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79843995</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79843995</sourcerecordid><originalsourceid>FETCH-LOGICAL-c332t-d13346bfebe7800c4994eefbec640cb2bff81f8c32c95007d99854505aa41d3b3</originalsourceid><addsrcrecordid>eNpFkEtLAzEUhYMoWh__QCEbX8jYm0kmnSyL-EJRwQruQpK5oZF51GQq-O9ttdTVWZxzvsVHyCGDSwZMDgFAZrli72cKzhVwAdnrBhkwUJDlDMQmGawnO2Q3pQ8AxoRU22Q7ZzKXMh-Q55d5DD4404eupZ2nhj6Zi-HDBR1PXkxCGtppsKHvIvWxa6jtYoWxDi3S6fcMY49tCl-YTumsNqkx-2TLmzrhwSr3yNvN9eTqLnt8vr2_Gj9mjvO8zyrGuZDWo8VRCeCEUgLRW3RSgLO59b5kvnQ8d6oAGFVKlYUooDBGsIpbvkdO_riz2H3OMfW6CclhXZsWu3nSI1UKrlSxGIq_oYtdShG9nsXQmPitGeilR72UpJeStAL961G_Lm5HK_7cNlitTytxi_541ZvkTO2jaV1I_2xVsELygv8AwSB6qA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79843995</pqid></control><display><type>article</type><title>Purification of a Na+/K+ ATPase inhibitor from borderline hypertensives' plasma</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>BOSCHI, S ; BORGHI, C ; MUNARINI, A ; VITTORIO COSTA, F ; ZHUOREN, L ; DEVRIES, D ; AMBROSIONI, E</creator><creatorcontrib>BOSCHI, S ; BORGHI, C ; MUNARINI, A ; VITTORIO COSTA, F ; ZHUOREN, L ; DEVRIES, D ; AMBROSIONI, E</creatorcontrib><description>Increasing experimental evidences suggest an involvement of an endogenous Na+/K+ ATPase inhibitor in regulating water and electrolytes balance as well as in the pathogenesis of hypertension. However, conflicting results on the nature and the chemical structure of this substance still make it difficult to understand exactly its physiological mechanism of action. In the present study an attempt was made to purify a Na+/K+ ATPase inhibitor from hypertensives' plasma by solid phase extraction followed by 2 HPLC steps using reverse and normal phase columns. The fractions, from both columns, were able to inhibit Na+/K+ ATPase, 3H-ouabain binding to enzyme, ouabain sensitive 86Rb uptake and pNPPase activity in a manner not affected by boiling. Ultrafiltration experiments demonstrate that inhibitory activity is largely due to a low-molecular weight substance. These findings seem to confirm the presence in hypertensives plasma of a Na+/K+ ATPase inhibitor with some similarities with ouabain.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(90)90340-S</identifier><identifier>PMID: 2162662</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Enzymes and enzyme inhibitors ; Erythrocytes - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolases ; Hypertension - blood ; Kinetics ; Ouabain - metabolism ; Protein Binding ; Rubidium - blood ; Sodium-Potassium-Exchanging ATPase - antagonists &amp; inhibitors ; Ultrafiltration</subject><ispartof>Biochemical and biophysical research communications, 1990-06, Vol.169 (2), p.360-368</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-d13346bfebe7800c4994eefbec640cb2bff81f8c32c95007d99854505aa41d3b3</citedby><cites>FETCH-LOGICAL-c332t-d13346bfebe7800c4994eefbec640cb2bff81f8c32c95007d99854505aa41d3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=19515635$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2162662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BOSCHI, S</creatorcontrib><creatorcontrib>BORGHI, C</creatorcontrib><creatorcontrib>MUNARINI, A</creatorcontrib><creatorcontrib>VITTORIO COSTA, F</creatorcontrib><creatorcontrib>ZHUOREN, L</creatorcontrib><creatorcontrib>DEVRIES, D</creatorcontrib><creatorcontrib>AMBROSIONI, E</creatorcontrib><title>Purification of a Na+/K+ ATPase inhibitor from borderline hypertensives' plasma</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Increasing experimental evidences suggest an involvement of an endogenous Na+/K+ ATPase inhibitor in regulating water and electrolytes balance as well as in the pathogenesis of hypertension. However, conflicting results on the nature and the chemical structure of this substance still make it difficult to understand exactly its physiological mechanism of action. In the present study an attempt was made to purify a Na+/K+ ATPase inhibitor from hypertensives' plasma by solid phase extraction followed by 2 HPLC steps using reverse and normal phase columns. The fractions, from both columns, were able to inhibit Na+/K+ ATPase, 3H-ouabain binding to enzyme, ouabain sensitive 86Rb uptake and pNPPase activity in a manner not affected by boiling. Ultrafiltration experiments demonstrate that inhibitory activity is largely due to a low-molecular weight substance. These findings seem to confirm the presence in hypertensives plasma of a Na+/K+ ATPase inhibitor with some similarities with ouabain.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Erythrocytes - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Hypertension - blood</subject><subject>Kinetics</subject><subject>Ouabain - metabolism</subject><subject>Protein Binding</subject><subject>Rubidium - blood</subject><subject>Sodium-Potassium-Exchanging ATPase - antagonists &amp; inhibitors</subject><subject>Ultrafiltration</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEUhYMoWh__QCEbX8jYm0kmnSyL-EJRwQruQpK5oZF51GQq-O9ttdTVWZxzvsVHyCGDSwZMDgFAZrli72cKzhVwAdnrBhkwUJDlDMQmGawnO2Q3pQ8AxoRU22Q7ZzKXMh-Q55d5DD4404eupZ2nhj6Zi-HDBR1PXkxCGtppsKHvIvWxa6jtYoWxDi3S6fcMY49tCl-YTumsNqkx-2TLmzrhwSr3yNvN9eTqLnt8vr2_Gj9mjvO8zyrGuZDWo8VRCeCEUgLRW3RSgLO59b5kvnQ8d6oAGFVKlYUooDBGsIpbvkdO_riz2H3OMfW6CclhXZsWu3nSI1UKrlSxGIq_oYtdShG9nsXQmPitGeilR72UpJeStAL961G_Lm5HK_7cNlitTytxi_541ZvkTO2jaV1I_2xVsELygv8AwSB6qA</recordid><startdate>19900615</startdate><enddate>19900615</enddate><creator>BOSCHI, S</creator><creator>BORGHI, C</creator><creator>MUNARINI, A</creator><creator>VITTORIO COSTA, F</creator><creator>ZHUOREN, L</creator><creator>DEVRIES, D</creator><creator>AMBROSIONI, E</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900615</creationdate><title>Purification of a Na+/K+ ATPase inhibitor from borderline hypertensives' plasma</title><author>BOSCHI, S ; BORGHI, C ; MUNARINI, A ; VITTORIO COSTA, F ; ZHUOREN, L ; DEVRIES, D ; AMBROSIONI, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-d13346bfebe7800c4994eefbec640cb2bff81f8c32c95007d99854505aa41d3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Erythrocytes - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Hypertension - blood</topic><topic>Kinetics</topic><topic>Ouabain - metabolism</topic><topic>Protein Binding</topic><topic>Rubidium - blood</topic><topic>Sodium-Potassium-Exchanging ATPase - antagonists &amp; inhibitors</topic><topic>Ultrafiltration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BOSCHI, S</creatorcontrib><creatorcontrib>BORGHI, C</creatorcontrib><creatorcontrib>MUNARINI, A</creatorcontrib><creatorcontrib>VITTORIO COSTA, F</creatorcontrib><creatorcontrib>ZHUOREN, L</creatorcontrib><creatorcontrib>DEVRIES, D</creatorcontrib><creatorcontrib>AMBROSIONI, E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BOSCHI, S</au><au>BORGHI, C</au><au>MUNARINI, A</au><au>VITTORIO COSTA, F</au><au>ZHUOREN, L</au><au>DEVRIES, D</au><au>AMBROSIONI, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of a Na+/K+ ATPase inhibitor from borderline hypertensives' plasma</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1990-06-15</date><risdate>1990</risdate><volume>169</volume><issue>2</issue><spage>360</spage><epage>368</epage><pages>360-368</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Increasing experimental evidences suggest an involvement of an endogenous Na+/K+ ATPase inhibitor in regulating water and electrolytes balance as well as in the pathogenesis of hypertension. However, conflicting results on the nature and the chemical structure of this substance still make it difficult to understand exactly its physiological mechanism of action. In the present study an attempt was made to purify a Na+/K+ ATPase inhibitor from hypertensives' plasma by solid phase extraction followed by 2 HPLC steps using reverse and normal phase columns. The fractions, from both columns, were able to inhibit Na+/K+ ATPase, 3H-ouabain binding to enzyme, ouabain sensitive 86Rb uptake and pNPPase activity in a manner not affected by boiling. Ultrafiltration experiments demonstrate that inhibitory activity is largely due to a low-molecular weight substance. These findings seem to confirm the presence in hypertensives plasma of a Na+/K+ ATPase inhibitor with some similarities with ouabain.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>2162662</pmid><doi>10.1016/0006-291X(90)90340-S</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0006-291X
ispartof Biochemical and biophysical research communications, 1990-06, Vol.169 (2), p.360-368
issn 0006-291X
1090-2104
language eng
recordid cdi_proquest_miscellaneous_79843995
source Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE
subjects Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid
Enzymes and enzyme inhibitors
Erythrocytes - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Hydrolases
Hypertension - blood
Kinetics
Ouabain - metabolism
Protein Binding
Rubidium - blood
Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors
Ultrafiltration
title Purification of a Na+/K+ ATPase inhibitor from borderline hypertensives' plasma
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T03%3A41%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20of%20a%20Na+/K+%20ATPase%20inhibitor%20from%20borderline%20hypertensives'%20plasma&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=BOSCHI,%20S&rft.date=1990-06-15&rft.volume=169&rft.issue=2&rft.spage=360&rft.epage=368&rft.pages=360-368&rft.issn=0006-291X&rft.eissn=1090-2104&rft.coden=BBRCA9&rft_id=info:doi/10.1016/0006-291X(90)90340-S&rft_dat=%3Cproquest_cross%3E79843995%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79843995&rft_id=info:pmid/2162662&rfr_iscdi=true