Developmental expression and location of IGF-I and IGF-II mRNA and protein in skeletal muscle
To investigate the role of IGF in muscle development in vivo, developmental expression and location of IGF-I and -II protein and mRNA were examined in fetal, postnatal, and adult skeletal muscle. Muscle tissue was collected from 30-, 44-, 59-, 68-, 75-, 89-, and 109-d porcine fetuses, 21-d neonatal...
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| Veröffentlicht in: | Journal of animal science 1998-04, Vol.76 (4), p.1004-1011 |
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| creator | Gerrard, D. E Okamura, C. S Ranalletta, M. A Grant, A. L |
| description | To investigate the role of IGF in muscle development in vivo, developmental expression and location of IGF-I and -II protein and mRNA were examined in fetal, postnatal, and adult skeletal muscle. Muscle tissue was collected from 30-, 44-, 59-, 68-, 75-, 89-, and 109-d porcine fetuses, 21-d neonatal pigs, and 6-mo-old (adult) pigs. Relative amounts of IGF-II mRNA peaked (P < .05) in 59-d fetal muscle and decreased thereafter. Inversely, muscle IGF-I expression increased (P < .05) to maximal levels around birth. For in situ hybridization, frozen muscle tissue sections (10 microm) were hybridized with a hydrolyzed form of the same riboprobes or incubated with polyclonal or monoclonal antibodies to IGF-I or -II, respectively. The majority of IGF-I and IGF-II mRNA was localized to developing muscle fibers, whereas little signal was found in the surrounding connective tissues. Immunofluorescent localization of IGF-I and -II confirmed that muscle IGF are present in developing muscle fibers. Collectively, these data show that IGF-I and -II are expressed and produced primarily in muscle cells within developing muscle tissue and support the hypothesis that IGF-I and -II modulate fetal muscle development. |
| doi_str_mv | 10.2527/1998.7641004x |
| format | Article |
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E ; Okamura, C. S ; Ranalletta, M. A ; Grant, A. L</creator><creatorcontrib>Gerrard, D. E ; Okamura, C. S ; Ranalletta, M. A ; Grant, A. L</creatorcontrib><description>To investigate the role of IGF in muscle development in vivo, developmental expression and location of IGF-I and -II protein and mRNA were examined in fetal, postnatal, and adult skeletal muscle. Muscle tissue was collected from 30-, 44-, 59-, 68-, 75-, 89-, and 109-d porcine fetuses, 21-d neonatal pigs, and 6-mo-old (adult) pigs. Relative amounts of IGF-II mRNA peaked (P < .05) in 59-d fetal muscle and decreased thereafter. Inversely, muscle IGF-I expression increased (P < .05) to maximal levels around birth. For in situ hybridization, frozen muscle tissue sections (10 microm) were hybridized with a hydrolyzed form of the same riboprobes or incubated with polyclonal or monoclonal antibodies to IGF-I or -II, respectively. The majority of IGF-I and IGF-II mRNA was localized to developing muscle fibers, whereas little signal was found in the surrounding connective tissues. Immunofluorescent localization of IGF-I and -II confirmed that muscle IGF are present in developing muscle fibers. Collectively, these data show that IGF-I and -II are expressed and produced primarily in muscle cells within developing muscle tissue and support the hypothesis that IGF-I and -II modulate fetal muscle development.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>EISSN: 0021-8812</identifier><identifier>DOI: 10.2527/1998.7641004x</identifier><identifier>PMID: 9581923</identifier><language>eng</language><publisher>Savoy, IL: Am Soc Animal Sci</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Northern - veterinary ; Female ; Fluorescent Antibody Technique, Indirect - veterinary ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - genetics ; Gestational Age ; Hogs ; In Situ Hybridization - veterinary ; Insulin-Like Growth Factor I - analysis ; Insulin-Like Growth Factor I - genetics ; Insulin-Like Growth Factor II - analysis ; Insulin-Like Growth Factor II - genetics ; Male ; Muscle, Skeletal - chemistry ; Muscle, Skeletal - embryology ; Muscular system ; Proteins ; Ribonucleic acid ; RNA ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Striated muscle. Tendons ; Swine - embryology ; Swine - growth & development ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of animal science, 1998-04, Vol.76 (4), p.1004-1011</ispartof><rights>1998 INIST-CNRS</rights><rights>Copyright American Society of Animal Science Apr 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-ca4abfbd634b6a132669df793d23437afa2a79d46aa7b6061ffdce8aebda1f603</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2221490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9581923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gerrard, D. E</creatorcontrib><creatorcontrib>Okamura, C. S</creatorcontrib><creatorcontrib>Ranalletta, M. A</creatorcontrib><creatorcontrib>Grant, A. L</creatorcontrib><title>Developmental expression and location of IGF-I and IGF-II mRNA and protein in skeletal muscle</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>To investigate the role of IGF in muscle development in vivo, developmental expression and location of IGF-I and -II protein and mRNA were examined in fetal, postnatal, and adult skeletal muscle. Muscle tissue was collected from 30-, 44-, 59-, 68-, 75-, 89-, and 109-d porcine fetuses, 21-d neonatal pigs, and 6-mo-old (adult) pigs. Relative amounts of IGF-II mRNA peaked (P < .05) in 59-d fetal muscle and decreased thereafter. Inversely, muscle IGF-I expression increased (P < .05) to maximal levels around birth. For in situ hybridization, frozen muscle tissue sections (10 microm) were hybridized with a hydrolyzed form of the same riboprobes or incubated with polyclonal or monoclonal antibodies to IGF-I or -II, respectively. The majority of IGF-I and IGF-II mRNA was localized to developing muscle fibers, whereas little signal was found in the surrounding connective tissues. Immunofluorescent localization of IGF-I and -II confirmed that muscle IGF are present in developing muscle fibers. Collectively, these data show that IGF-I and -II are expressed and produced primarily in muscle cells within developing muscle tissue and support the hypothesis that IGF-I and -II modulate fetal muscle development.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern - veterinary</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Gestational Age</subject><subject>Hogs</subject><subject>In Situ Hybridization - veterinary</subject><subject>Insulin-Like Growth Factor I - analysis</subject><subject>Insulin-Like Growth Factor I - genetics</subject><subject>Insulin-Like Growth Factor II - analysis</subject><subject>Insulin-Like Growth Factor II - genetics</subject><subject>Male</subject><subject>Muscle, Skeletal - chemistry</subject><subject>Muscle, Skeletal - embryology</subject><subject>Muscular system</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Striated muscle. Tendons</subject><subject>Swine - embryology</subject><subject>Swine - growth & development</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0021-8812</issn><issn>1525-3163</issn><issn>0021-8812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkN9r1TAUx4Mo8zp99FEoIr515neaxzHdvDAURB8lnKYnrte0vSatzv_e9O46RAgkJ-fDNycfQp4zesYVN2-Ytc2Z0ZJRKm8fkA1TXNWCafGQbCjlrG4axh-TJznvKGVcWXVCTqxqmOViQ76-xZ8Yp_2A4wyxwtt9wpz7aaxg7Ko4eZjXYgrV9uqy3h5uD6dtNXz6cH6o92masR-rsvJ3jLgGDUv2EZ-SRwFixmfH_ZR8uXz3-eJ9ff3xantxfl17YdRce5DQhrbTQrYamOBa2y4YKzoupDAQgIOxndQAptVUsxA6jw1g2wELmopT8vout4zyY8E8u6HPHmOEEaclO2MbyY0SBXz5H7ibljSW2RxnxZOVihWovoN8mnJOGNw-9QOk345Rtzp3q3P313nhXxxDl3bA7p4-Si79V8c-ZA8xJBh9n-8xzjmT9p9P3PTfbn71CV0eIMYSytwOstFOuvVB8QclA5Tn</recordid><startdate>19980401</startdate><enddate>19980401</enddate><creator>Gerrard, D. E</creator><creator>Okamura, C. S</creator><creator>Ranalletta, M. A</creator><creator>Grant, A. 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E ; Okamura, C. S ; Ranalletta, M. A ; Grant, A. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-ca4abfbd634b6a132669df793d23437afa2a79d46aa7b6061ffdce8aebda1f603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern - veterinary</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - genetics</topic><topic>Gestational Age</topic><topic>Hogs</topic><topic>In Situ Hybridization - veterinary</topic><topic>Insulin-Like Growth Factor I - analysis</topic><topic>Insulin-Like Growth Factor I - genetics</topic><topic>Insulin-Like Growth Factor II - analysis</topic><topic>Insulin-Like Growth Factor II - genetics</topic><topic>Male</topic><topic>Muscle, Skeletal - chemistry</topic><topic>Muscle, Skeletal - embryology</topic><topic>Muscular system</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Striated muscle. Tendons</topic><topic>Swine - embryology</topic><topic>Swine - growth & development</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gerrard, D. 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E</au><au>Okamura, C. S</au><au>Ranalletta, M. A</au><au>Grant, A. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental expression and location of IGF-I and IGF-II mRNA and protein in skeletal muscle</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>1998-04-01</date><risdate>1998</risdate><volume>76</volume><issue>4</issue><spage>1004</spage><epage>1011</epage><pages>1004-1011</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><eissn>0021-8812</eissn><abstract>To investigate the role of IGF in muscle development in vivo, developmental expression and location of IGF-I and -II protein and mRNA were examined in fetal, postnatal, and adult skeletal muscle. Muscle tissue was collected from 30-, 44-, 59-, 68-, 75-, 89-, and 109-d porcine fetuses, 21-d neonatal pigs, and 6-mo-old (adult) pigs. Relative amounts of IGF-II mRNA peaked (P < .05) in 59-d fetal muscle and decreased thereafter. Inversely, muscle IGF-I expression increased (P < .05) to maximal levels around birth. For in situ hybridization, frozen muscle tissue sections (10 microm) were hybridized with a hydrolyzed form of the same riboprobes or incubated with polyclonal or monoclonal antibodies to IGF-I or -II, respectively. The majority of IGF-I and IGF-II mRNA was localized to developing muscle fibers, whereas little signal was found in the surrounding connective tissues. Immunofluorescent localization of IGF-I and -II confirmed that muscle IGF are present in developing muscle fibers. Collectively, these data show that IGF-I and -II are expressed and produced primarily in muscle cells within developing muscle tissue and support the hypothesis that IGF-I and -II modulate fetal muscle development.</abstract><cop>Savoy, IL</cop><pub>Am Soc Animal Sci</pub><pmid>9581923</pmid><doi>10.2527/1998.7641004x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of animal science, 1998-04, Vol.76 (4), p.1004-1011 |
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| source | MEDLINE; Oxford Journals |
| subjects | Animals Biological and medical sciences Blotting, Northern - veterinary Female Fluorescent Antibody Technique, Indirect - veterinary Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - genetics Gestational Age Hogs In Situ Hybridization - veterinary Insulin-Like Growth Factor I - analysis Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor II - analysis Insulin-Like Growth Factor II - genetics Male Muscle, Skeletal - chemistry Muscle, Skeletal - embryology Muscular system Proteins Ribonucleic acid RNA RNA, Messenger - analysis RNA, Messenger - genetics Striated muscle. Tendons Swine - embryology Swine - growth & development Vertebrates: osteoarticular system, musculoskeletal system |
| title | Developmental expression and location of IGF-I and IGF-II mRNA and protein in skeletal muscle |
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